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学科主题: 临床医学
题名:
Identification of miRNA-21 and miRNA-24 in plasma as potential early stage markers of acute cerebral infarction
作者: Zhou, Jiansuo; Zhang, Jie
关键词: microRNA-21 ; microRNA-24 ; oxygen-glucose deprivation ; N2A neuroblastoma cells ; acute cerebral infarction
刊名: MOLECULAR MEDICINE REPORTS
发表日期: 2014-08-01
DOI: 10.3892/mmr.2014.2245
卷: 10, 期:2, 页:971-976
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Medicine, Research & Experimental
研究领域[WOS]: Oncology ; Research & Experimental Medicine
关键词[WOS]: CIRCULATING MICRORNAS ; CANCER CELLS ; BIOMARKERS ; EXPRESSION ; APOPTOSIS ; MIR-21 ; ISCHEMIA ; DISEASES ; BRAIN ; SERUM
英文摘要:

This study investigated the role of microRNA-21 (miR-21) and microRNA-24 (miR-24) in the pathological processes that follow cerebral ischemic injury and examined the potential use of miR-21 and -24 in stroke diagnostics as sensitive plasma biomarkers. An oxygen-glucose deprivation (OGD) model was constructed using mouse N2A neuroblastoma cells (N2A). Western blot analysis and quantitative polymerase chain reaction (qPCR) were employed to detect protein and miRNA expression levels. miR-21 and miR-24 were analyzed in the plasma from 68 patients with acute cerebral infarction (ACT) and 21 healthy individuals. In the present study, it was identified that plasma miR-21 and miR-24 were lower in ACT patients than in the controls (P<0.05). A positive correlation was demonstrated between plasma miR-21 and miR-24, and a negative correlation was revealed between miR-21, miR-24 and the National Institutes of Health Scales Score (NIHSS) within the first day following stroke. In addition, the expression of miR-21 and miR-24 was upregulated by 3.3- and 4.9-fold, respectively, when the reoxygenation time persisted up to 24 h following 3 h of OGD. The expression of Bcl-2 was upregulated following gain of miR-21 function, while X-linked inhibitor of apoptosis protein (XIAP) was downregulated after gain of miR-24 function in N2A cells. The data suggested that miR-21 may have an antiapoptotic effect in N2A neuroblastoma cells following OGD and reoxygenation, while miR-24 may have a pro-apoptotic effect. Therefore, these microRNAs may be potential therapeutic targets for the treatment of post-ischemic injury and may act as diagnostic markers during the early stage of ACI.

语种: 英语
所属项目编号: 2012BAI37B01
项目资助者: National Key Technology RD Program
WOS记录号: WOS:000339127600060
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52335
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: Peking Univ, Dept Lab Med, Hosp 3, Beijing 100191, Peoples R China

Recommended Citation:
Zhou, Jiansuo,Zhang, Jie. Identification of miRNA-21 and miRNA-24 in plasma as potential early stage markers of acute cerebral infarction[J]. MOLECULAR MEDICINE REPORTS,2014,10(2):971-976.
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