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学科主题临床医学
Identification of miRNA-21 and miRNA-24 in plasma as potential early stage markers of acute cerebral infarction
Zhou, Jiansuo; Zhang, Jie
关键词Microrna-21 Microrna-24 Oxygen-glucose Deprivation N2a Neuroblastoma Cells Acute Cerebral Infarction
刊名MOLECULAR MEDICINE REPORTS
2014-08-01
DOI10.3892/mmr.2014.2245
10期:2页:971-976
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Medicine, Research & Experimental
研究领域[WOS]Oncology ; Research & Experimental Medicine
关键词[WOS]CIRCULATING MICRORNAS ; CANCER CELLS ; BIOMARKERS ; EXPRESSION ; APOPTOSIS ; MIR-21 ; ISCHEMIA ; DISEASES ; BRAIN ; SERUM
英文摘要

This study investigated the role of microRNA-21 (miR-21) and microRNA-24 (miR-24) in the pathological processes that follow cerebral ischemic injury and examined the potential use of miR-21 and -24 in stroke diagnostics as sensitive plasma biomarkers. An oxygen-glucose deprivation (OGD) model was constructed using mouse N2A neuroblastoma cells (N2A). Western blot analysis and quantitative polymerase chain reaction (qPCR) were employed to detect protein and miRNA expression levels. miR-21 and miR-24 were analyzed in the plasma from 68 patients with acute cerebral infarction (ACT) and 21 healthy individuals. In the present study, it was identified that plasma miR-21 and miR-24 were lower in ACT patients than in the controls (P<0.05). A positive correlation was demonstrated between plasma miR-21 and miR-24, and a negative correlation was revealed between miR-21, miR-24 and the National Institutes of Health Scales Score (NIHSS) within the first day following stroke. In addition, the expression of miR-21 and miR-24 was upregulated by 3.3- and 4.9-fold, respectively, when the reoxygenation time persisted up to 24 h following 3 h of OGD. The expression of Bcl-2 was upregulated following gain of miR-21 function, while X-linked inhibitor of apoptosis protein (XIAP) was downregulated after gain of miR-24 function in N2A cells. The data suggested that miR-21 may have an antiapoptotic effect in N2A neuroblastoma cells following OGD and reoxygenation, while miR-24 may have a pro-apoptotic effect. Therefore, these microRNAs may be potential therapeutic targets for the treatment of post-ischemic injury and may act as diagnostic markers during the early stage of ACI.

语种英语
WOS记录号WOS:000339127600060
项目编号2012BAI37B01
资助机构National Key Technology RD Program
引用统计
被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52335
专题北京大学第三临床医学院
北京大学第三临床医学院_检验科
作者单位Peking Univ, Dept Lab Med, Hosp 3, Beijing 100191, Peoples R China
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Zhou, Jiansuo,Zhang, Jie. Identification of miRNA-21 and miRNA-24 in plasma as potential early stage markers of acute cerebral infarction[J]. MOLECULAR MEDICINE REPORTS,2014,10(2):971-976.
APA Zhou, Jiansuo,&Zhang, Jie.(2014).Identification of miRNA-21 and miRNA-24 in plasma as potential early stage markers of acute cerebral infarction.MOLECULAR MEDICINE REPORTS,10(2),971-976.
MLA Zhou, Jiansuo,et al."Identification of miRNA-21 and miRNA-24 in plasma as potential early stage markers of acute cerebral infarction".MOLECULAR MEDICINE REPORTS 10.2(2014):971-976.
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