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学科主题: 基础医学
题名:
Phylogenetic analysis of the full genome of rabbit hepatitis E virus (rbHEV) and molecular biologic study on the possibility of cross species transmission of rbHEV
作者: Geng, Jiabao2; Fu, Hongwei3; Wang, Ling1; Bu, Qiuning1; Liu, Peng1; Wang, Maorong2; Sui, Yunhua2; Wang, Xiaojuan1; Zhu, Yonghong1; Zhuang, Hui1
关键词: Rabbit hepatitis E virus (rbHEV) ; Phylogentic analysis ; Host range ; Genotype
刊名: INFECTION GENETICS AND EVOLUTION
发表日期: 2011-12-01
DOI: 10.1016/j.meegid.2011.09.006
卷: 11, 期:8, 页:2020-2025
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Infectious Diseases
研究领域[WOS]: Infectious Diseases
关键词[WOS]: INFECTION
英文摘要:

Rabbit HEV strains were isolated in China from farmed rabbits, which may be a reservoir of a novel genotype of HEV. However, there is little information regarding host range and zoonotic potential of rabbit HEV. A previous study from this group identified 25 specific nucleotide positions as possibly being involved in specifying the host range of HEV and/or in determining the severity of hepatitis E disease, whereas this previous analysis did not extend to the new rabbit HEVs. Consequently the complete genome of the rabbit HEV strain ch-bj-n1 was amplified as overlapping fragments using a combination of reverse-transcription-nested polymerase chain reaction and rapid amplification of cDNA ends. The resulting sequence together with two other rabbit HEVs were compared with 91 other HEV isolates representative of genotypes 1-4. Phylogenetic analysis showed that the novel rabbit HEV strain should be classified into a new HEV genotype which has a closer evolutionary relationship with genotype 3 than genotypes 1,2 and 4, with 20 of the 25 specific nucleotide positions identified as being present in rabbit HEV genome. Alpha-helix, hydrophilicity, and surface probability plots together with antigenic index analysis of the ORF2 protein were all undertaken to investigate the antigenicity of rabbit HEV. The alpha-helix plot and antigenicity index of the rabbit ORF2 protein was nearly identical to that of genotype 1 HEV isolates from human. Furthermore, 20 possible host range determinants in rabbit HEV were identical to those isolates from zoonotic groups, which may suggest a potential possibility of cross species transmission of rabbit HEV. (C) 2011 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 30771996
项目资助者: National Science Foundation of China
WOS记录号: WOS:000298774500023
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52341
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: 1.PLA, Inst Hepatol, Hosp 81, Nanjing, Jiangsu, Peoples R China
2.Peking Univ, Dept Microbiol, Hlth Sci Ctr, Beijing 100191, Peoples R China
3.Tianjin Med Univ Gen Hosp, Dept Clin Lab Med, Tianjin 300052, Peoples R China

Recommended Citation:
Geng, Jiabao,Fu, Hongwei,Wang, Ling,et al. Phylogenetic analysis of the full genome of rabbit hepatitis E virus (rbHEV) and molecular biologic study on the possibility of cross species transmission of rbHEV[J]. INFECTION GENETICS AND EVOLUTION,2011,11(8):2020-2025.
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