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学科主题基础医学
Phylogenetic analysis of the full genome of rabbit hepatitis E virus (rbHEV) and molecular biologic study on the possibility of cross species transmission of rbHEV
Geng, Jiabao2; Fu, Hongwei3; Wang, Ling1; Bu, Qiuning1; Liu, Peng1; Wang, Maorong2; Sui, Yunhua2; Wang, Xiaojuan1; Zhu, Yonghong1; Zhuang, Hui1
关键词Rabbit hepatitis E virus (rbHEV) Phylogentic analysis Host range Genotype
刊名INFECTION GENETICS AND EVOLUTION
2011-12-01
DOI10.1016/j.meegid.2011.09.006
11期:8页:2020-2025
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Infectious Diseases
资助者National Science Foundation of China ; National Science Foundation of China
研究领域[WOS]Infectious Diseases
关键词[WOS]INFECTION
英文摘要

Rabbit HEV strains were isolated in China from farmed rabbits, which may be a reservoir of a novel genotype of HEV. However, there is little information regarding host range and zoonotic potential of rabbit HEV. A previous study from this group identified 25 specific nucleotide positions as possibly being involved in specifying the host range of HEV and/or in determining the severity of hepatitis E disease, whereas this previous analysis did not extend to the new rabbit HEVs. Consequently the complete genome of the rabbit HEV strain ch-bj-n1 was amplified as overlapping fragments using a combination of reverse-transcription-nested polymerase chain reaction and rapid amplification of cDNA ends. The resulting sequence together with two other rabbit HEVs were compared with 91 other HEV isolates representative of genotypes 1-4. Phylogenetic analysis showed that the novel rabbit HEV strain should be classified into a new HEV genotype which has a closer evolutionary relationship with genotype 3 than genotypes 1,2 and 4, with 20 of the 25 specific nucleotide positions identified as being present in rabbit HEV genome. Alpha-helix, hydrophilicity, and surface probability plots together with antigenic index analysis of the ORF2 protein were all undertaken to investigate the antigenicity of rabbit HEV. The alpha-helix plot and antigenicity index of the rabbit ORF2 protein was nearly identical to that of genotype 1 HEV isolates from human. Furthermore, 20 possible host range determinants in rabbit HEV were identical to those isolates from zoonotic groups, which may suggest a potential possibility of cross species transmission of rabbit HEV. (C) 2011 Elsevier B.V. All rights reserved.

语种英语
所属项目编号30771996
资助者National Science Foundation of China ; National Science Foundation of China
WOS记录号WOS:000298774500023
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52341
Collection北京大学基础医学院_病原生物学系
作者单位1.PLA, Inst Hepatol, Hosp 81, Nanjing, Jiangsu, Peoples R China
2.Peking Univ, Dept Microbiol, Hlth Sci Ctr, Beijing 100191, Peoples R China
3.Tianjin Med Univ Gen Hosp, Dept Clin Lab Med, Tianjin 300052, Peoples R China
Recommended Citation
GB/T 7714
Geng, Jiabao,Fu, Hongwei,Wang, Ling,et al. Phylogenetic analysis of the full genome of rabbit hepatitis E virus (rbHEV) and molecular biologic study on the possibility of cross species transmission of rbHEV[J]. INFECTION GENETICS AND EVOLUTION,2011,11(8):2020-2025.
APA Geng, Jiabao.,Fu, Hongwei.,Wang, Ling.,Bu, Qiuning.,Liu, Peng.,...&Zhuang, Hui.(2011).Phylogenetic analysis of the full genome of rabbit hepatitis E virus (rbHEV) and molecular biologic study on the possibility of cross species transmission of rbHEV.INFECTION GENETICS AND EVOLUTION,11(8),2020-2025.
MLA Geng, Jiabao,et al."Phylogenetic analysis of the full genome of rabbit hepatitis E virus (rbHEV) and molecular biologic study on the possibility of cross species transmission of rbHEV".INFECTION GENETICS AND EVOLUTION 11.8(2011):2020-2025.
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