学科主题基础医学
Molecular cloning and characterization of chemokine-like factor super family member 1 (CKLFSF1), a novel human gene with at least 23 alternative splicing isoforms in testis tissue
Wang, L1; Wu, CX1; Zheng, Y1; Qiu, XY1; Wang, L1; Fan, H1; Han, WL1; Lv, BF1; Wang, Y1; Zhu, XH1; Xu, MX1; Ding, PG1; Cheng, SH1; Zhang, YM1; Song, QS1; Ma, DL1
关键词CKLF testis alternative splicing CKLFSF1
刊名INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
2004-08-01
DOI10.1016/j.biocel.2003.11.017
36期:8页:1492-1501
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]PROTEIN-KINASE ; IDENTIFICATION ; SPERMATOGENESIS ; EXPRESSION ; COMPUTER ; SEQUENCE ; SUBUNIT
英文摘要

Chemokine-like factor (CKLF) was isolated from PHA-stimulated U937 cells. It is composed of 152 amino acids and located on chromosome 16q22. Utilizing bioinformatics, based on CKLF cDNA and protein sequences, in combination with experimental validation, we identified a novel gene designated chemokine-like factor super family member 1 (CKLFSF1). CKLFSF1 maps on chromosome 16q22, and the full-length gene comprises of seven exons and six introns. Using RACE-PCR, we identified two potential alternative transcription start sites, 1A and 1B. Northern blot and RT-PCR analysis demonstrated that CKLFSF1 is predominantly expressed in human testis tissue, with only lower levels of expression in many other human tissues. RT-PCR and cDNA sequencing identified 23 alternatively spliced isoforms of CKLFSF1 in the testis tissue, which encode protein variants ranging from 36 to 169 amino acids in length. Immunohistochemistry analysis demonstrated that CKLFSF1 proteins are highly expressed in spermatocyte and in tissue fluid of human testes tissue. In light of these findings, we propose that CKLFSF1 may play an important role in spermatogenesis or testicular development. (C) 2004 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000222204400015
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52352
专题北京大学基础医学院_北京大学人类疾病基因研究中心
作者单位1.Peking Univ, Ctr Human Dis Genom, Beijing 100083, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Lab Med Immunol, Beijing 100083, Peoples R China
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GB/T 7714
Wang, L,Wu, CX,Zheng, Y,et al. Molecular cloning and characterization of chemokine-like factor super family member 1 (CKLFSF1), a novel human gene with at least 23 alternative splicing isoforms in testis tissue[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2004,36(8):1492-1501.
APA Wang, L.,Wu, CX.,Zheng, Y.,Qiu, XY.,Wang, L.,...&Ma, DL.(2004).Molecular cloning and characterization of chemokine-like factor super family member 1 (CKLFSF1), a novel human gene with at least 23 alternative splicing isoforms in testis tissue.INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,36(8),1492-1501.
MLA Wang, L,et al."Molecular cloning and characterization of chemokine-like factor super family member 1 (CKLFSF1), a novel human gene with at least 23 alternative splicing isoforms in testis tissue".INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 36.8(2004):1492-1501.
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