北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 北京大学人类疾病基因研究中心  > 期刊论文
学科主题: 基础医学
题名:
Molecular cloning and characterization of chemokine-like factor super family member 1 (CKLFSF1), a novel human gene with at least 23 alternative splicing isoforms in testis tissue
作者: Wang, L1; Wu, CX1; Zheng, Y1; Qiu, XY1; Wang, L1; Fan, H1; Han, WL1; Lv, BF1; Wang, Y1; Zhu, XH1; Xu, MX1; Ding, PG1; Cheng, SH1; Zhang, YM1; Song, QS1; Ma, DL1
关键词: CKLF ; testis ; alternative splicing ; CKLFSF1
刊名: INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
发表日期: 2004-08-01
DOI: 10.1016/j.biocel.2003.11.017
卷: 36, 期:8, 页:1492-1501
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: PROTEIN-KINASE ; IDENTIFICATION ; SPERMATOGENESIS ; EXPRESSION ; COMPUTER ; SEQUENCE ; SUBUNIT
英文摘要:

Chemokine-like factor (CKLF) was isolated from PHA-stimulated U937 cells. It is composed of 152 amino acids and located on chromosome 16q22. Utilizing bioinformatics, based on CKLF cDNA and protein sequences, in combination with experimental validation, we identified a novel gene designated chemokine-like factor super family member 1 (CKLFSF1). CKLFSF1 maps on chromosome 16q22, and the full-length gene comprises of seven exons and six introns. Using RACE-PCR, we identified two potential alternative transcription start sites, 1A and 1B. Northern blot and RT-PCR analysis demonstrated that CKLFSF1 is predominantly expressed in human testis tissue, with only lower levels of expression in many other human tissues. RT-PCR and cDNA sequencing identified 23 alternatively spliced isoforms of CKLFSF1 in the testis tissue, which encode protein variants ranging from 36 to 169 amino acids in length. Immunohistochemistry analysis demonstrated that CKLFSF1 proteins are highly expressed in spermatocyte and in tissue fluid of human testes tissue. In light of these findings, we propose that CKLFSF1 may play an important role in spermatogenesis or testicular development. (C) 2004 Elsevier Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000222204400015
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52352
Appears in Collections:基础医学院_北京大学人类疾病基因研究中心_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Molecular cloning and characterization of chemokine-like factor super family member 1 (CKLFSF1), a novel human gene with at least 23 alternative splicing isoforms in testis tissue.pdf(356KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Peking Univ, Ctr Human Dis Genom, Beijing 100083, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Lab Med Immunol, Beijing 100083, Peoples R China

Recommended Citation:
Wang, L,Wu, CX,Zheng, Y,et al. Molecular cloning and characterization of chemokine-like factor super family member 1 (CKLFSF1), a novel human gene with at least 23 alternative splicing isoforms in testis tissue[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2004,36(8):1492-1501.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang, L]'s Articles
[Wu, CX]'s Articles
[Zheng, Y]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang, L]‘s Articles
[Wu, CX]‘s Articles
[Zheng, Y]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace