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学科主题: 临床医学
题名:
Development of neonatal mouse and fetal human testicular tissue as ectopic grafts in immunodeficient mice
作者: Yu, J1; Cai, ZM1; Wan, HJ1; Zhang, FT1; Ye, J1; Fang, JZ1; Gui, YT1; Ye, JX1
关键词: allograft ; germ cells ; spermatogenesis ; testis ; xenograft
刊名: ASIAN JOURNAL OF ANDROLOGY
发表日期: 2006-07-01
DOI: 10.1111/j.1745-7262.2006.00189.x
卷: 8, 期:4, 页:393-403
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Andrology ; Urology & Nephrology
研究领域[WOS]: Endocrinology & Metabolism ; Urology & Nephrology
关键词[WOS]: GERM-CELL TRANSPLANTATION ; SPERMATOGENIC CELLS ; MALE-INFERTILITY ; PROTEIN-KINASE ; DIFFERENTIATION ; TESTIS ; SPERM ; GENE ; EXPRESSION ; GENERATION
英文摘要:

Aim: To investigate the stepwise development and germ cell gene expression in allografted neonatal mouse testes and the differentiation of immature human testicular cells in xenografted human testes. Methods: Immunodeficient nude mice were used as hosts for allografting of neonatal mouse testes and xenografting of human fetal testicular tissues. Stepwise development and stage-specific gene expression of germ cells in allografts were systematically evaluated and parallel compared with those in intact mice by periodically monitoring the graft status with measurement of graft weight, histological analysis and determination of five stage-specific genes. Human testicular tissues from 20 and 26 weeks fetuses were used for the xenografting study. Histological analysis of xenografts was performed 116 and 135 d after the grafting procedure. Results: In the allografting study, progressive increase in tissue volume and weight as well as in tubule diameter in grafts was observed; the appearance time of various germ cells in seminiferous tubules, including spermatogonia, spermatocytes, round and elongate spermatids and sperm, was comparable with that in intact donors; the initiation of gene transcription in grafts showed a similar trend as in normal mice. Graft weight ceased to increase after 7-8 weeks and degradation of grafts was observed after 5 weeks with progressive damage to seminiferous epithelium. In the xenografting study using immature human testicular tissues, graft survival and development was indicated by increasing graft weight, Sertoli cells differentiation into advanced stage, germ cells migration and location to the basal lamina and formation of a niche-like structure. Conclusion: The developmental course and gene expression pattern of germ cells in allografts were similar to those in intact mice. The best time point for retrieval of mouse sperm from grafts was 5-7 weeks after grafting procedure. An accelerated development of immature human testicular cells could be achieved by ectopic xenografting of human testes.

语种: 英语
WOS记录号: WOS:000238484900002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52358
Appears in Collections:北京大学深圳医院_中心实验室_期刊论文

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作者单位: 1.Peking Univ, Shenzhen Hosp, Cent Lab, Shenzhen 518036, Peoples R China
2.Peking Univ, Shenzhen Hosp, Lab Male Reprod, Shenzhen 518036, Peoples R China

Recommended Citation:
Yu, J,Cai, ZM,Wan, HJ,et al. Development of neonatal mouse and fetal human testicular tissue as ectopic grafts in immunodeficient mice[J]. ASIAN JOURNAL OF ANDROLOGY,2006,8(4):393-403.
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