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Homocysteine increases the synthesis of adrenomedullin in vascular fibroblasts of rats
Gao, L1; Wang, DY1; Li, Y1; Bu, DF1; Chang, L1; Pang, YZ1; Qi, YF1; Tang, CS1
关键词Homocysteine Aorta Fibroblasts Adrenomedullin Mrna Oxide Stress
刊名CHINESE SCIENCE BULLETIN
2003-05-01
DOI10.1360/02wc0400
48期:10页:983-987
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]PEPTIDE ; CELLS ; GENE
英文摘要

The effect of homocysteine (Hcy) on the synthesis of adrenomedullin (ADM) was examined in vascular fibroblasts of rats to explore the possible activity of ADM in cardiovascular diseases. ADM from cultured vascular fibroblasts was measured by Radioimmunoassay (RIA) and was characterized by reverse phase high performance liquid chromatography. ADM mRNA in the fibroblasts was quantitated by competitive RT-PCR. The synthesis of ADM from vascular fibroblasts was increased by Hcy in a concentration dependent manner. ADM content in cultured medium increased by 68%-150% (P < 0.01, as compared to the control) when the fibroblasts were incubated in 50-200 mumol (.) L-1 Hcy for 12 h, and the content increased by 55 %-98% (P < 0.01) if the incubation lasted for 24 h. Hydrogen peroxide treatment also increased the synthesis of ADM and it promoted ADM synthesis synergetically with Hcy. The changes of ADM content were partially or completely inhibited by the antioxidant N-acetylcysteine (NAC) treatment. Hcy induced the increase of ADM mRNA and the amount of ADM mRNA was increased by 78% as compared with control (P < 0.05). NAC almost completely inhibited the increase of ADM mRNA induced by Hcy. In conclusion, Hcy treatment up-regulated the expression of ADM gene and increased the synthesis of ADM in vascular fibroblasts. Oxidative stress may be involved in the processes of the ADM changes induce by Hcy.

语种英语
WOS记录号WOS:000183349600010
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52365
专题北京大学第一临床医学院_心血管内科
作者单位1.Peking Univ, Hosp 1, Inst Cardiovasc Dis Res, Beijing 100034, Peoples R China
2.Peking Univ, Dept Physiol, Hlth Sci Ctr, Beijing 100034, Peoples R China
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GB/T 7714
Gao, L,Wang, DY,Li, Y,et al. Homocysteine increases the synthesis of adrenomedullin in vascular fibroblasts of rats[J]. CHINESE SCIENCE BULLETIN,2003,48(10):983-987.
APA Gao, L.,Wang, DY.,Li, Y.,Bu, DF.,Chang, L.,...&Tang, CS.(2003).Homocysteine increases the synthesis of adrenomedullin in vascular fibroblasts of rats.CHINESE SCIENCE BULLETIN,48(10),983-987.
MLA Gao, L,et al."Homocysteine increases the synthesis of adrenomedullin in vascular fibroblasts of rats".CHINESE SCIENCE BULLETIN 48.10(2003):983-987.
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