|Correlation between the different chain lengths of free fatty acid oxidation and ability of trophoblastic invasion|
|Yu Huan1; Yang Zi1; Ding Xiaoyan1; Wang Yanling2; Han Yiwei1|
|关键词||Fatty Acid Beta-oxidation Long Chain Fatty Acid Trophoblasts Invasion|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||EARLY GESTATIONAL STAGE ; PREECLAMPSIA ; CYTOTROPHOBLASTS ; PATHOGENESIS ; MODEL|
Background Preeclampsia (PE) is associated with abnormal fatty acid beta-oxidation (FAO), especially metabolic disorders of long-chain fatty acid oxidation. The role of FAO dysfunction in inadequate invasion is unclear. The aim of this study was to explore the influence of various lengths fatty acids oxidation on invasiveness of trophoblasts.
Methods Primary human trophoblast cells and HTR8/SVneo cells were treated with fatty acids of various lengths. Morphological changes, lipid deposition and ultrastructure changes of trophoblast cells were detected. Cells invasiveness was determined by transwell insert. CPT1, CPT2 and long-chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD) protein expression were analyzed. The correlation between intracellular lipid droplets deposition and cells invasiveness was evaluated.
Results Cells treated with long-chain fatty acids showed significant increased lipid droplets deposition, severe mitochondrial damage, decreased CPT2 and LCHAD protein expression (P <0.05) but no significant difference in CPT1 protein expression (P >0.05). Invasiveness of the trophoblast cells of the LC-FFA group significantly decreased (P <0.05). Intracellular lipid droplets deposition was negatively correlated with invasivenss (R=-0.745, P <0.05).
Conclusion Trophoblast cells after stimulation with long chain fatty acids exist fatty acid oxidation disorders, and reduce the ability of trophoblastic invasion.
|项目编号||81241021 ; 7132215|
|资助机构||National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation|
|作者单位||1.Peking Univ, Dept Obstet & Gynecol, Hosp 3, Beijing 100191, Peoples R China|
2.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
|Yu Huan,Yang Zi,Ding Xiaoyan,et al. Correlation between the different chain lengths of free fatty acid oxidation and ability of trophoblastic invasion[J]. CHINESE MEDICAL JOURNAL,2014,127(19):3378-3382.|
|APA||Yu Huan,Yang Zi,Ding Xiaoyan,Wang Yanling,&Han Yiwei.(2014).Correlation between the different chain lengths of free fatty acid oxidation and ability of trophoblastic invasion.CHINESE MEDICAL JOURNAL,127(19),3378-3382.|
|MLA||Yu Huan,et al."Correlation between the different chain lengths of free fatty acid oxidation and ability of trophoblastic invasion".CHINESE MEDICAL JOURNAL 127.19(2014):3378-3382.|