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学科主题: 临床医学
题名:
Correlation between the different chain lengths of free fatty acid oxidation and ability of trophoblastic invasion
作者: Yu Huan1; Yang Zi1; Ding Xiaoyan1; Wang Yanling2; Han Yiwei1
关键词: fatty acid beta-oxidation ; long chain fatty acid ; trophoblasts ; invasion
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2014-10-05
DOI: 10.3760/cma.j.issn.0366-6999.20141020
卷: 127, 期:19, 页:3378-3382
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: EARLY GESTATIONAL STAGE ; PREECLAMPSIA ; CYTOTROPHOBLASTS ; PATHOGENESIS ; MODEL
英文摘要:

Background Preeclampsia (PE) is associated with abnormal fatty acid beta-oxidation (FAO), especially metabolic disorders of long-chain fatty acid oxidation. The role of FAO dysfunction in inadequate invasion is unclear. The aim of this study was to explore the influence of various lengths fatty acids oxidation on invasiveness of trophoblasts.

Methods Primary human trophoblast cells and HTR8/SVneo cells were treated with fatty acids of various lengths. Morphological changes, lipid deposition and ultrastructure changes of trophoblast cells were detected. Cells invasiveness was determined by transwell insert. CPT1, CPT2 and long-chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD) protein expression were analyzed. The correlation between intracellular lipid droplets deposition and cells invasiveness was evaluated.

Results Cells treated with long-chain fatty acids showed significant increased lipid droplets deposition, severe mitochondrial damage, decreased CPT2 and LCHAD protein expression (P <0.05) but no significant difference in CPT1 protein expression (P >0.05). Invasiveness of the trophoblast cells of the LC-FFA group significantly decreased (P <0.05). Intracellular lipid droplets deposition was negatively correlated with invasivenss (R=-0.745, P <0.05).

Conclusion Trophoblast cells after stimulation with long chain fatty acids exist fatty acid oxidation disorders, and reduce the ability of trophoblastic invasion.

语种: 英语
所属项目编号: 81241021 ; 7132215
项目资助者: National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation
WOS记录号: WOS:000344724000005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52374
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Peking Univ, Dept Obstet & Gynecol, Hosp 3, Beijing 100191, Peoples R China
2.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China

Recommended Citation:
Yu Huan,Yang Zi,Ding Xiaoyan,et al. Correlation between the different chain lengths of free fatty acid oxidation and ability of trophoblastic invasion[J]. CHINESE MEDICAL JOURNAL,2014,127(19):3378-3382.
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