IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
Correlation between the different chain lengths of free fatty acid oxidation and ability of trophoblastic invasion
Yu Huan1; Yang Zi1; Ding Xiaoyan1; Wang Yanling2; Han Yiwei1
关键词Fatty Acid Beta-oxidation Long Chain Fatty Acid Trophoblasts Invasion
刊名CHINESE MEDICAL JOURNAL
2014-10-05
DOI10.3760/cma.j.issn.0366-6999.20141020
127期:19页:3378-3382
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]EARLY GESTATIONAL STAGE ; PREECLAMPSIA ; CYTOTROPHOBLASTS ; PATHOGENESIS ; MODEL
英文摘要

Background Preeclampsia (PE) is associated with abnormal fatty acid beta-oxidation (FAO), especially metabolic disorders of long-chain fatty acid oxidation. The role of FAO dysfunction in inadequate invasion is unclear. The aim of this study was to explore the influence of various lengths fatty acids oxidation on invasiveness of trophoblasts.

Methods Primary human trophoblast cells and HTR8/SVneo cells were treated with fatty acids of various lengths. Morphological changes, lipid deposition and ultrastructure changes of trophoblast cells were detected. Cells invasiveness was determined by transwell insert. CPT1, CPT2 and long-chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD) protein expression were analyzed. The correlation between intracellular lipid droplets deposition and cells invasiveness was evaluated.

Results Cells treated with long-chain fatty acids showed significant increased lipid droplets deposition, severe mitochondrial damage, decreased CPT2 and LCHAD protein expression (P <0.05) but no significant difference in CPT1 protein expression (P >0.05). Invasiveness of the trophoblast cells of the LC-FFA group significantly decreased (P <0.05). Intracellular lipid droplets deposition was negatively correlated with invasivenss (R=-0.745, P <0.05).

Conclusion Trophoblast cells after stimulation with long chain fatty acids exist fatty acid oxidation disorders, and reduce the ability of trophoblastic invasion.

语种英语
WOS记录号WOS:000344724000005
项目编号81241021 ; 7132215
资助机构National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52374
专题北京大学第三临床医学院
作者单位1.Peking Univ, Dept Obstet & Gynecol, Hosp 3, Beijing 100191, Peoples R China
2.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
推荐引用方式
GB/T 7714
Yu Huan,Yang Zi,Ding Xiaoyan,et al. Correlation between the different chain lengths of free fatty acid oxidation and ability of trophoblastic invasion[J]. CHINESE MEDICAL JOURNAL,2014,127(19):3378-3382.
APA Yu Huan,Yang Zi,Ding Xiaoyan,Wang Yanling,&Han Yiwei.(2014).Correlation between the different chain lengths of free fatty acid oxidation and ability of trophoblastic invasion.CHINESE MEDICAL JOURNAL,127(19),3378-3382.
MLA Yu Huan,et al."Correlation between the different chain lengths of free fatty acid oxidation and ability of trophoblastic invasion".CHINESE MEDICAL JOURNAL 127.19(2014):3378-3382.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yu Huan]的文章
[Yang Zi]的文章
[Ding Xiaoyan]的文章
百度学术
百度学术中相似的文章
[Yu Huan]的文章
[Yang Zi]的文章
[Ding Xiaoyan]的文章
必应学术
必应学术中相似的文章
[Yu Huan]的文章
[Yang Zi]的文章
[Ding Xiaoyan]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。