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学科主题: 临床医学
题名:
Chromosomal characteristics at cleavage and blastocyst stages from the same embryos
作者: Huang, Jin1,2,3; Zhao, Nan1,2,3; Wang, Xiaozhu4; Qiao, Jie1,2,3; Liu, Ping1,2,3
关键词: Comparative genomic hybridization ; Single nucleotide polymorphism ; Embryo development ; Chromosome
刊名: JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
发表日期: 2015-05-01
DOI: 10.1007/s10815-015-0450-1
卷: 32, 期:5, 页:781-787
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Genetics & Heredity ; Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]: Genetics & Heredity ; Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]: COMPARATIVE GENOMIC HYBRIDIZATION ; ADVANCED MATERNAL AGE ; SELF-CORRECTION ; POLAR BODIES ; ANEUPLOIDY ; BIOPSY ; PGD ; ABNORMALITIES ; AMPLIFICATION ; MOSAICISM
英文摘要:

To investigate chromosomal characteristics in the same embryos at cleavage and blastocyst stage.

Six PGD/PGS cycles were retrospective studied, including five chromosomal translocation PGD cycles and one recurrent abortion PGS cycle. The cleavage embryos were biopsied one blastomere at day 3, followed by blastocyst culture. Trophectoderm cell biopsy was performed when embryos developed into the blastocyst stage. Whole genome amplification and 24-chromosomal analysis by CGH/SNP microarray was performed on each blastomere and trophectoderm cells.

After PGD/PGS, only one couple had no euploid embryos to transfer. Five couples delivered a healthy, balanced-karyotype baby. A total of 18 embryos had both blastomere and trophectoderm cell reliable results available. Of the 18 embryos, eleven embryos contained identical chromosomes from cleavage stage to blastocyst stage and six embryos contained almost identical chromosomes . Only one embryo presented opposite chromosomal results for the cleavage and blastocyst stage, with abnormal chromosomes in the blastomere but normal chromosomes in trophectoderm cells.

Most embryos maintain chromosomal stability during embryonic development. Compared to cleavage stage, blastocyst stage may provide more reliable aneuploidy results.

语种: 英语
所属项目编号: 81070534
项目资助者: Peking-Tsinghua Center for Life Sciences ; National Science Foundation of China
WOS记录号: WOS:000354484900015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52390
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: 1.Minist Educ, Key Lab Assisted Reprod, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100191, Peoples R China
3.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing 100191, Peoples R China
4.Peking Univ, Sch Basic Med Sci, Dept Genet, Beijing 100191, Peoples R China

Recommended Citation:
Huang, Jin,Zhao, Nan,Wang, Xiaozhu,et al. Chromosomal characteristics at cleavage and blastocyst stages from the same embryos[J]. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS,2015,32(5):781-787.
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