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Use of proteomic analysis of endometriosis to identify different protein expression in patients with endometriosis versus normal controls
Zhang, Hong; Niu, Yidong; Feng, Jie; Guo, Huifang; Ye, Xue; Cui, Heng
关键词Endometriosis Two-dimensional Electrophoresis Proteomics Mass Spectrometry
刊名FERTILITY AND STERILITY
2006-08-01
DOI10.1016/j.fertnstert.2006.01.028
86期:2页:274-282
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]DENTIN MATRIX PROTEIN-1 ; DISEASE ; ACTIN ; TRANSCRIPTION ; ANTIBODIES ; CANCER ; TISSUE ; CELLS
英文摘要

Objective: To use proteomic techniques, including two-dimensional electrophores (2-DE), Western blot, and mass spectrometry, to screen and identify proteins that were expressed differently in patients with endometriosis versus normal controls.

Design: First, we aimed to find a difference in the way serum and eutropic endometrial proteins were expressed in women with and without endometriosis. Second, we were interested in searching for endometriotic proteins, which were specifically recognized by sera from patients with endometriosis.

Setting: Collaborative investigation in an academic research environment.

Patient(s): Consenting women of reproductive age taking no medications and with laparascopically proven endometriosis.

Intervention(s): Surgical excision of eutopic and ectopic endometrial biopsy and phlebotomization of patients with endometriosis and controls.

Main Outcome Measure(s): Protein expression.

Result(s): Thirteen protein spots from serum correlated with 11 known proteins and 11 protein spots from endometrium correlated with 11 known proteins were found differently expressed between women with and without endometriosis. Some proteins may be cytoskeletons, and some may be involved in the regulation of cell cycle, signal transduction, or immunological function. Three proteins, which were identified as vimentin, beta-actin, and ATP synthase beta subunit, hybridized significantly differently between endometriosis sera and normal sera.

Conclusion(s): The data help to establish a human endometriosis proteome database and broaden our understanding of the pathogenesis of endometriosis. Further study of the proteins identified herein will assist in the eventual development of new diagnoses and treatments for endometriosisd.

语种英语
WOS记录号WOS:000239696700002
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被引频次:68[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52409
专题北京大学第二临床医学院_妇科肿瘤中心
作者单位Peking Univ, Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Hong,Niu, Yidong,Feng, Jie,et al. Use of proteomic analysis of endometriosis to identify different protein expression in patients with endometriosis versus normal controls[J]. FERTILITY AND STERILITY,2006,86(2):274-282.
APA Zhang, Hong,Niu, Yidong,Feng, Jie,Guo, Huifang,Ye, Xue,&Cui, Heng.(2006).Use of proteomic analysis of endometriosis to identify different protein expression in patients with endometriosis versus normal controls.FERTILITY AND STERILITY,86(2),274-282.
MLA Zhang, Hong,et al."Use of proteomic analysis of endometriosis to identify different protein expression in patients with endometriosis versus normal controls".FERTILITY AND STERILITY 86.2(2006):274-282.
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