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学科主题: 临床医学
题名:
Use of proteomic analysis of endometriosis to identify different protein expression in patients with endometriosis versus normal controls
作者: Zhang, Hong; Niu, Yidong; Feng, Jie; Guo, Huifang; Ye, Xue; Cui, Heng
关键词: endometriosis ; two-dimensional electrophoresis ; proteomics ; mass spectrometry
刊名: FERTILITY AND STERILITY
发表日期: 2006-08-01
DOI: 10.1016/j.fertnstert.2006.01.028
卷: 86, 期:2, 页:274-282
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]: Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]: DENTIN MATRIX PROTEIN-1 ; DISEASE ; ACTIN ; TRANSCRIPTION ; ANTIBODIES ; CANCER ; TISSUE ; CELLS
英文摘要:

Objective: To use proteomic techniques, including two-dimensional electrophores (2-DE), Western blot, and mass spectrometry, to screen and identify proteins that were expressed differently in patients with endometriosis versus normal controls.

Design: First, we aimed to find a difference in the way serum and eutropic endometrial proteins were expressed in women with and without endometriosis. Second, we were interested in searching for endometriotic proteins, which were specifically recognized by sera from patients with endometriosis.

Setting: Collaborative investigation in an academic research environment.

Patient(s): Consenting women of reproductive age taking no medications and with laparascopically proven endometriosis.

Intervention(s): Surgical excision of eutopic and ectopic endometrial biopsy and phlebotomization of patients with endometriosis and controls.

Main Outcome Measure(s): Protein expression.

Result(s): Thirteen protein spots from serum correlated with 11 known proteins and 11 protein spots from endometrium correlated with 11 known proteins were found differently expressed between women with and without endometriosis. Some proteins may be cytoskeletons, and some may be involved in the regulation of cell cycle, signal transduction, or immunological function. Three proteins, which were identified as vimentin, beta-actin, and ATP synthase beta subunit, hybridized significantly differently between endometriosis sera and normal sera.

Conclusion(s): The data help to establish a human endometriosis proteome database and broaden our understanding of the pathogenesis of endometriosis. Further study of the proteins identified herein will assist in the eventual development of new diagnoses and treatments for endometriosisd.

语种: 英语
WOS记录号: WOS:000239696700002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52409
Appears in Collections:北京大学第二临床医学院_妇科肿瘤中心_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China

Recommended Citation:
Zhang, Hong,Niu, Yidong,Feng, Jie,et al. Use of proteomic analysis of endometriosis to identify different protein expression in patients with endometriosis versus normal controls[J]. FERTILITY AND STERILITY,2006,86(2):274-282.
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