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学科主题: 基础医学
题名:
A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus, a Chinese medicinal herb
作者: Shao, BM1; Xu, W1; Dai, H1; Tu, PF1; Li, ZJ1; Gao, XM1
关键词: Astragalus membranaceus ; polysaccharides ; receptor ; TLR4 ; immune cells
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2004-08-06
DOI: 10.1016/j.bbrc.2004.06.065
卷: 320, 期:4, 页:1103-1111
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: TOLL-LIKE RECEPTOR-4 ; B-CELLS ; PLATYCODON-GRANDIFLORUM ; BETA-GLUCANS ; ACTIVATION ; RECOGNITION ; MACROPHAGES ; LIPOPOLYSACCHARIDE ; CULTURE ; ANTITUMOR
英文摘要:

The immunopotentiating effect of the roots of Astragalus membranaceus, a medicinal herb, has been associated with its polysaccharide fractions (Astragalus polysaccharides, APS). We herein demonstrate that APS activates mouse B cells and macrophages, but not T cells. in terms of proliferation or cytokine production. Fluorescence-labeled APS (fl-APS) was able to selectively stain murine B cells. macrophages and a also human tumor cell line, THP-1, as determined in flow cytometric analysis and confocal laser scanning microscopy. The specific binding of APS to B cells and macrophages was competitively inhibited by bacterial lipopolysaccharides. Rabbit-anti-mouse immunoglobulin (Ig) antibody was able to inhibit APS-induced proliferation of, and APS binding to, mouse B cells. Additionally, APS effectively stimulated the proliferation of splenic B cells from C3H/HeJ mice that have a mutated TLR4 molecule incapable of signal transduction. These results indicate that APS activates B cells via membrane Ig in a TLR4-independent manner. Interestingly, macrophages from C3H/HeJ mice were unable to respond to APS stimulation, suggesting a positive involvement of the TLR4 molecule in APS-mediated macrophage activation. Monoclonal Ab against mouse TLR4 partially inhibited APS binding with macrophages, implying direct interaction between APS and TLR4 on cell surface. These results may have important implications for our understanding on the molecular mechanisms of immunopotentiating polysaccharides from medicinal herbs. (C) 2004 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000222923400009
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52410
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Peking Univ, Ctr Hlth Sci, Sch Basic Med Sci, Dept Immunol, Beijing, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Nat Sci, Beijing, Peoples R China
3.Peking Univ, Ctr Hlth Sci, Sch Pharmaceut Sci, Dept Organ Chem, Beijing, Peoples R China

Recommended Citation:
Shao, BM,Xu, W,Dai, H,et al. A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus, a Chinese medicinal herb[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2004,320(4):1103-1111.
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