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A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus, a Chinese medicinal herb
Shao, BM1; Xu, W1; Dai, H1; Tu, PF1; Li, ZJ1; Gao, XM1
关键词Astragalus membranaceus polysaccharides receptor TLR4 immune cells
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2004-08-06
DOI10.1016/j.bbrc.2004.06.065
320期:4页:1103-1111
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]TOLL-LIKE RECEPTOR-4 ; B-CELLS ; PLATYCODON-GRANDIFLORUM ; BETA-GLUCANS ; ACTIVATION ; RECOGNITION ; MACROPHAGES ; LIPOPOLYSACCHARIDE ; CULTURE ; ANTITUMOR
英文摘要

The immunopotentiating effect of the roots of Astragalus membranaceus, a medicinal herb, has been associated with its polysaccharide fractions (Astragalus polysaccharides, APS). We herein demonstrate that APS activates mouse B cells and macrophages, but not T cells. in terms of proliferation or cytokine production. Fluorescence-labeled APS (fl-APS) was able to selectively stain murine B cells. macrophages and a also human tumor cell line, THP-1, as determined in flow cytometric analysis and confocal laser scanning microscopy. The specific binding of APS to B cells and macrophages was competitively inhibited by bacterial lipopolysaccharides. Rabbit-anti-mouse immunoglobulin (Ig) antibody was able to inhibit APS-induced proliferation of, and APS binding to, mouse B cells. Additionally, APS effectively stimulated the proliferation of splenic B cells from C3H/HeJ mice that have a mutated TLR4 molecule incapable of signal transduction. These results indicate that APS activates B cells via membrane Ig in a TLR4-independent manner. Interestingly, macrophages from C3H/HeJ mice were unable to respond to APS stimulation, suggesting a positive involvement of the TLR4 molecule in APS-mediated macrophage activation. Monoclonal Ab against mouse TLR4 partially inhibited APS binding with macrophages, implying direct interaction between APS and TLR4 on cell surface. These results may have important implications for our understanding on the molecular mechanisms of immunopotentiating polysaccharides from medicinal herbs. (C) 2004 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000222923400009
引用统计
被引频次:220[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52410
专题北京大学基础医学院_免疫学系
北京大学药学院_天然药物学系
北京大学第三临床医学院_心血管内科
北京大学口腔医学院_口腔正畸科
作者单位1.Peking Univ, Ctr Hlth Sci, Sch Basic Med Sci, Dept Immunol, Beijing, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Nat Sci, Beijing, Peoples R China
3.Peking Univ, Ctr Hlth Sci, Sch Pharmaceut Sci, Dept Organ Chem, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Shao, BM,Xu, W,Dai, H,et al. A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus, a Chinese medicinal herb[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2004,320(4):1103-1111.
APA Shao, BM,Xu, W,Dai, H,Tu, PF,Li, ZJ,&Gao, XM.(2004).A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus, a Chinese medicinal herb.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,320(4),1103-1111.
MLA Shao, BM,et al."A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus, a Chinese medicinal herb".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 320.4(2004):1103-1111.
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