学科主题基础医学
Effects of homocysteine on murine splenic B lymphocyte proliferation and its signal transduction mechanism
Zhang, Q1; Zeng, XK1; Guo, JX1; Wang, X1
关键词Atherosclerosis Free Radicals Immunology Infection/inflammation Signal Transduction
刊名CARDIOVASCULAR RESEARCH
2001-11-01
52期:2页:328-336
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cardiac & Cardiovascular Systems
研究领域[WOS]Cardiovascular System & Cardiology
关键词[WOS]FACTOR-KAPPA-B ; TYROSINE PHOSPHORYLATION ; ATHEROSCLEROTIC PLAQUES ; HYDROGEN-PEROXIDE ; RISK FACTOR ; CELLS ; GROWTH ; INVOLVEMENT ; GENERATION ; ACTIVATION
英文摘要

Objective: Elevated plasma homocysteine (Hey) levels have been defined as an increased risk of atherosclerosis, However, the mechanisms that Hey induces the development of atherosclerosis a-re not fully understood. Therefore, effect of Hey on B lymphocyte proliferation and its cellular mechanism were examined in normal and hyperhomocysteinemia ApoE-knockout mice. Methods: Mouse B lymphocytes were incubated with Hey, related compounds and/or antioxidants and/or inhibitors of PKC, p38 MAPK, NF-kappaB in the presence or absence of lipopolysaccharide. DNA synthesis, production of reactive oxygen species was measured. Results: Hey (0.1-3.0 mM) and other compounds with thiol (-SH), such as cysteine and glutathione significantly increased resting and lipopolysaccharide-induced B lymphocyte proliferation. ApoE-knockout mice with hypercysteinemia (plasma Hey levels were 20.3+/-2.9 vs. 2.6+/-0.6 muM in control, P<0.05) had a significant promotion of B cell proliferation in response to lipopolysaccharide. Hey also increased intracellular reactive oxygen species production, Radical scavengers reduced Hcy-induced B lymphocyte proliferation. The promotion of Hcy was significantly inhibited by inhibitors of PKC (calphostin C and RO-31-82201), p39 MAPK (SB 202190 and PD 169316) and NF-kappaB (pyrrolidine dithiocarbamate). Conclusions: The reactive oxygen species generated by thiol (-SH) auto-oxidation of Hey are essential, and PKC, p38 MAPK and NF-kappaB are involved in the Hey-induced B lymphocyte proliferation. Hyperhomocysteinemia may increase B lymphocyte susceptibility to inflammatory progression of atherosclerotic lesions. (C) 2001 Elsevier Science B.V. All rights reserved.

语种英语
WOS记录号WOS:000175037800021
引用统计
被引频次:29[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52443
专题北京大学基础医学院_生理学与病理生理学系
作者单位1.Third Hosp, Inst Vasc Med, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Physiol, Beijing 100083, Peoples R China
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GB/T 7714
Zhang, Q,Zeng, XK,Guo, JX,et al. Effects of homocysteine on murine splenic B lymphocyte proliferation and its signal transduction mechanism[J]. CARDIOVASCULAR RESEARCH,2001,52(2):328-336.
APA Zhang, Q,Zeng, XK,Guo, JX,&Wang, X.(2001).Effects of homocysteine on murine splenic B lymphocyte proliferation and its signal transduction mechanism.CARDIOVASCULAR RESEARCH,52(2),328-336.
MLA Zhang, Q,et al."Effects of homocysteine on murine splenic B lymphocyte proliferation and its signal transduction mechanism".CARDIOVASCULAR RESEARCH 52.2(2001):328-336.
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