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学科主题: 临床医学
题名:
Screening and identification of the up-regulated genes in human mesangial cells exposed to angiotensin II
作者: Liang, XB; Zhang, H; Zhou, AY; Hou, P; Wang, HY
关键词: angiotensin II ; gene cloning ; mesangial cells ; extracellular matrix
刊名: HYPERTENSION RESEARCH
发表日期: 2003-03-01
卷: 26, 期:3, 页:225-235
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Peripheral Vascular Disease
研究领域[WOS]: Cardiovascular System & Cardiology
关键词[WOS]: SUPPRESSION SUBTRACTIVE HYBRIDIZATION ; GROWTH-FACTOR-BETA ; FIBRONECTIN BIOSYNTHESIS ; MATRIX PROTEINS ; MESSENGER-RNA ; EXPRESSION ; RAT ; TRANSCRIPTION ; ENDOTHELIN-1 ; HYPERTENSION
英文摘要:

Accumulation of extracellular matrix (ECM) in the glomerular mesangium is a common feature of many progressive renal diseases. Angiotensin II (Ang II) plays a major role in the progression of chronic kidney diseases in part by induction of ECM. However, the precise molecular signals responsible for this effect are unknown. To explore possible molecular mechanisms of ECM production related to Ang II, we screened and identified genes up-regulated by Ang II in cultured human mesangial cells (MC). Detection of up-regulated genes was determined by mRNA populations from human MC with and without Ang II stimulation (10(-6)mol/l, 24 h) by suppression subtractive hybridization. Reverse Northern blot analysis was performed to screen for differentially expressed genes. Full-length cDNAs of three novel genes were isolated by rapid amplification of cDNA ends (RACE)-polymerase chain reaction (PCR). One of these novel genes, AngRem104, was further investigated by Northern blot, Western blot and reverse transcription (RT)-PCR. The bioinformatics analysis implied that AngRem104 coded for a nuclear protein that was widely expressed in various normal human tissues. Moreover, up-regulation of AngRem104 induced by Ang II was time-dependent and was dose-dependently blocked by the Ang If type 1 receptor antagonist, Losartan. Interestingly, we also demonstrated that AngReam104 was associated with increased fibronectin expression. We conclude that AngRem104 is a novel human gene that is related to the expression of fibronectin and that is up-regulated by Ang II in human MC. These findings may lead to new insights into the mechanisms of glomerular sclerosis associated with Ang II.

语种: 英语
WOS记录号: WOS:000181849800005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52454
Appears in Collections:北京大学第一临床医学院_肾脏内科_期刊论文

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作者单位: Peking Univ, Inst Nephrol, Div Renal, Hosp 1, Beijing 100034, Peoples R China

Recommended Citation:
Liang, XB,Zhang, H,Zhou, AY,et al. Screening and identification of the up-regulated genes in human mesangial cells exposed to angiotensin II[J]. HYPERTENSION RESEARCH,2003,26(3):225-235.
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