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学科主题临床医学
ELL is an HIF-1 alpha Partner That Regulates and Responds to Hypoxia Response in PC3 Cells
Liu, Lingqi1,2,3; Ai, Junkui3; Xiao, Wuhan4; Liu, June3; Wang, Yujuan5; Xin, Dianqi1,2; He, Zhisong1,2; Guo, Yinglu1,2; Wang, Zhou3,6,7
关键词Ell Hypoxia Hif-1 Alpha Prostate Cancer
刊名PROSTATE
2010-05-15
DOI10.1002/pros.21113
70期:7页:797-805
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Endocrinology & Metabolism ; Urology & Nephrology
资助者NIH ; NIH
研究领域[WOS]Endocrinology & Metabolism ; Urology & Nephrology
关键词[WOS]ELONGATION-FACTOR ELL ; TUMOR-SUPPRESSOR PROTEIN ; PROSTATE-CANCER ; INDUCIBLE FACTOR-1 ; MYELOID-LEUKEMIA ; GROWTH ; POLYMERASE ; HIF-1 ; GENE ; T(11-19)(Q23-P13.1)
英文摘要

BACKGROUND. Eleven nineteen lysine-rich leukemia (ELL) plays an important role in tumorigenesis and animal development. HIF-1 is a transcriptional factor that functions as a master regulator of O(2) homeostasis. Our previous studies showed that a binding partner of ELL, U19/Eaf2, can modulate HIF-1 alpha activity and hypoxia response, suggesting that ELL may also influence HIF-1 alpha pathway and hypoxia response.

METHODS. Co-localization and co-immunoprecipitation were performed to test the interaction between ELL and HIF-1 alpha. PC3 cells with stable ELL knockdown and PC3 cells with stable ELL overexpression, along with their controls, were established using lentiviral expression system. Western blot and real-time PCR were performed to test the effect of ELL on HIF-1 alpha protein and its down-stream gene transcription. To elucidate potential effect of hypoxia on ELL, cell growth and colony formation assays were performed using PC3 subline with stable ELL overexpression.

RESULTS. ELL is associated with HIF-1 alpha in transfected cells. In PC3 prostate cancer cells, ELL inhibited HIF-1 alpha protein level and down-stream gene expression. As expected, ELL inhibited cell growth and colony formation under normoxia. Interestingly, the inhibition was alleviated under hypoxia.

CONCLUSIONS. Our findings suggest that ELL and HIF-1 alpha are binding partners and can modulate the functions of each other in hypoxia. Prostate 70: 797-805, 2010. (C) 2010 Wiley-Liss, Inc.

语种英语
所属项目编号R37 DK51193 ; R01 CA120386 ; P50 CA90386
资助者NIH ; NIH
WOS记录号WOS:000277338800012
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52458
Collection北京大学第一临床医学院_泌尿外科
作者单位1.Peking Univ, Hosp 1, Dept Urol, Beijing 100034, Peoples R China
2.Peking Univ, Inst Urol, Beijing 100034, Peoples R China
3.Univ Pittsburgh, Sch Med, Dept Urol, Pittsburgh, PA 15232 USA
4.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
5.Univ Pittsburgh, Sch Med, Dept Pathol, Pittsburgh, PA 15232 USA
6.Univ Pittsburgh, Sch Med, Dept Pharmacol & Chem Biol, Pittsburgh, PA 15232 USA
7.Univ Pittsburgh, Sch Med, Inst Canc, Pittsburgh, PA 15232 USA
Recommended Citation
GB/T 7714
Liu, Lingqi,Ai, Junkui,Xiao, Wuhan,et al. ELL is an HIF-1 alpha Partner That Regulates and Responds to Hypoxia Response in PC3 Cells[J]. PROSTATE,2010,70(7):797-805.
APA Liu, Lingqi.,Ai, Junkui.,Xiao, Wuhan.,Liu, June.,Wang, Yujuan.,...&Wang, Zhou.(2010).ELL is an HIF-1 alpha Partner That Regulates and Responds to Hypoxia Response in PC3 Cells.PROSTATE,70(7),797-805.
MLA Liu, Lingqi,et al."ELL is an HIF-1 alpha Partner That Regulates and Responds to Hypoxia Response in PC3 Cells".PROSTATE 70.7(2010):797-805.
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