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学科主题: 临床医学
题名:
Growth and activation of PI-3K/PKB and Akt by stromal cell-derived factor 1 alpha in endometrial carcinoma cells with expression of suppressor endoprotein PTEN
作者: Li, XP; Zhao, D; Gao, M; Zhao, C; Wang, JL; Wei, LH
关键词: endometrial carcinoma ; stromal cell derived factor-1 alpha
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2006-03-05
卷: 119, 期:5, 页:378-383
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: CXCR4 EXPRESSION ; BLADDER-CANCER ; RECEPTOR ; MUTATIONS ; MIGRATION ; OVARIAN ; CXCL12
英文摘要:

Background Mutation or deletion in the phosphatase and tensin homologue deleted on chromosome ten (PTEN) gene has been identified as an important cause of endometrial carcinoma; stromal cell derived factor-1 alpha (SDF-1 alpha) exerts growth-promoting effects on endometrial cancer cells through activation of the PI-3 kinase/Akt pathway and downstream effectors such as extracellular-responsive kinase (ERK). In this study, a plasmid containing the PTEN gene was transfected into Ishikawa cells to investigate the difference in growth and signal transduction between Ishikawa-PTEN and Ishikawa cells after SDF-1 alpha stimulation, and to study mechanisms of the involvement of PTEN protein in endometrial carcinoma development.

Methods Ishikawa cells were transfected with a plasmid (pLXSN-PTEN) containing the PTEN gene and a plasmid (pLXSN-EGFP) with enhanced green fluorescent protein (EGFP). Cells were then screened to obtain Ishikawa-PTEN cells and Ishikawa-neo cells that can both stably express PTEN protein and EGFR Expression of PTEN protein, phosphorylation levels of AKT and ERK (pAKT and pERK) and growth differences in Ishikawa-PTEN, Ishikawa-neo and Ishikawa cells before and after SDF-alpha stimulation were then determined by Western blots and MTT assays.

Results Western blot analysis showed that Ishikawa cells produced PTEN after transfection with the PTEN gene. At 15 minutes after SDF-1 alpha stimulation, the pAKT level of Ishikawa-PTEN cells was lower than that of Ishikawa-neo cells and Ishikawa cells. There was no significant difference in pERK levels among the three cell lines. The positive effect of SDF-1 alpha on Ishikawa-PTEN cells growth was markedly less than the effect on Ishikawa-neo and Ishikawa cells. However, in the absence of SDF-1 alpha stimulation (baseline), the pAKT level in Ishikawa-PTEN cells was less than that in Ishikawa cells. There was a significant difference in growth between the Ishikawa-PTEN cells and the Ishikawa-neo cells.

Conclusions PTEN gene transfection can regulate the level of pAKT but not pERK in Ishikawa-PTEN cells. PTEN protein may suppress the growth-promoting effect of SDF-1 alpha on endometrial carcinoma by inhibiting the PI-3K/AKT signal transduction pathway.

语种: 英语
WOS记录号: WOS:000235990600005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52473
Appears in Collections:北京大学第二临床医学院_妇科肿瘤中心_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China

Recommended Citation:
Li, XP,Zhao, D,Gao, M,et al. Growth and activation of PI-3K/PKB and Akt by stromal cell-derived factor 1 alpha in endometrial carcinoma cells with expression of suppressor endoprotein PTEN[J]. CHINESE MEDICAL JOURNAL,2006,119(5):378-383.
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