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学科主题: 临床医学
题名:
Real-time detection of alpha(1A)-AR movement stimulated by phenylephrine in single living cells
作者: Xu, Ning1; Liang, Zhang-yi1; Xu, Ming1; Guan, Ying-hua1; He, Qi-hua1; Han, Qi-de1; Zhao, Xin-sheng1; Zhang, You-yi1
关键词: adrenergic receptor ; trajectory ; living cell
刊名: ACTA PHARMACOLOGICA SINICA
发表日期: 2007-06-01
DOI: 10.1111/j.1745-7254.2007.00545.x
卷: 28, 期:6, 页:796-802
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: ALPHA(1)-ADRENOCEPTOR SUBTYPES ; LOCALIZATION ; RECEPTOR ; DESENSITIZATION ; INFECTION
英文摘要:

Aim: To investigate the movement of alpha(1A)-adrenergic receptors(alpha(1A)-AR) stimulated by agonist, phenylephrine (PE), and the dynamics of receptor movement in real time in single living cells with millisecond resolution. Methods: We labeled alpha(1A)-AR using the monoclonal, anti-FLAG (a kind of tag) antibody and Cy3-conju-gated goat anti-mouse IgG and recorded the trajectory of their transport process in living HEK293A cells stimulated by agonist, PE, and then analyzed their dynamic properties. Results: The specific detection of alpha(1A)-AR on the surface of living HEK293A-alpha(1A) cells was achieved. alpha(1A)-AR internalize under the stimulation of PE. After the cells were stimulated with PE for 20 min, apparent colocalization was found between alpha(1A)-AR and F-actins. After 40 min stimulation of PE, trajectories of approximate linear motion in HEK293A-alpha(1A) cells were recorded, and their velocity was calculated. Conclusion: The specific labeling method on the living cell surface provides a convenient means of real-time detection of the behavior of surface receptors. By this method we were able to specifically detect alpha(1A)-AR and record the behavior of individual particles of receptors with 50 ms exposure time in real time in single living cells.

语种: 英语
WOS记录号: WOS:000246756300007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52485
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: 1.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China
2.Peking Univ, Third Hosp, Inst Vasc Med, Beijing 100083, Peoples R China
3.Peking Univ, Coll Chem & Mol Engn, Dept Biol Chem, Beijing 100871, Peoples R China
4.Peking Univ, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem & Unstable & Stable Spe, Beijing 100871, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Ctr Med Anal, Beijing 100083, Peoples R China

Recommended Citation:
Xu, Ning,Liang, Zhang-yi,Xu, Ming,et al. Real-time detection of alpha(1A)-AR movement stimulated by phenylephrine in single living cells[J]. ACTA PHARMACOLOGICA SINICA,2007,28(6):796-802.
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