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Targeting of Integrin-Linked Kinase with Small Interfering RNA Inhibits VEGF-Induced Angiogenesis in Retinal Endothelial Cells
Xie, Wankun2; Zhao, Min1; Zhou, Weiyan3; Guo, Lili1; Huang, Lvzhen1; Yu, Wenzhen1; Li, Xiaoxin1
关键词Integrin-linked Kinase Vascular Endothelial Growth Factor Retinal Vascular Endothelial Cells Retinal Neovascularization
刊名OPHTHALMIC RESEARCH
2013
DOI10.1159/000345070
49期:3页:139-149
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]EXPERIMENTAL PROLIFERATIVE VITREORETINOPATHY ; GROWTH-FACTOR ; EYE DISEASE ; MIGRATION ; CANCER ; ACTIVATION ; RECEPTORS ; BIOLOGY
英文摘要

Background: The pathological angiogenesis in the retina is a major cause of vision loss at all ages. Vascular endothelial growth factor (VEGF) has been reported as the most potent inducer of retinal neovascularization. We previously demonstrated that integrin-linked kinase (ILK) regulates retinal vascular endothelial proliferation, migration and tube formation. However, little is known about the existence of cross-talk between ILK and VEGF signaling in retinal vascular endothelial cells and the probable regulatory role of ILK during VEGF-induced retinal endothelial cell migration. The purpose of this work was to investigate the role of ILK in VEGF-induced retinal neovascularization. Methods: Cultured retinal endothelial cells (RF/6A) were knocked down for ILK using a small interfering RNA (siRNA). For this, cellular ILK expression was quantified by real-time quantitative PCR, Western blot analysis and immunocytochemical assay, and cytotoxicity of transfection was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. ILK siRNA-transfected RF/6A cells were induced by VEGF, and cell proliferation was determined by the MTT assay, cell migration was measured by cell counting in modified Boyden chambers and cell spreading and tube formation assays were performed. Furthermore, the impact of ILK-specific siRNA on VEGF-induced VEGF receptor 2 (VEGFR-2) phosphorylation and activation of downstream signal pathways were tested by Western blot analysis. Results: Both ILK mRNA and protein levels were virtually undetectable after transfection with ILK siRNA, and blocking the expression of ILK by siRNA significantly inhibited VEGF-induced retinal endothelial cell proliferation, attachment, spreading, migration and tube formation. Knockdown of ILK effectively suppressed VEGF-induced p38 mitogen-activated protein kinase (MAPK) and Akt phosphorylation, but had no effects on VEGFR-2, extracellular signal-regulated protein kinase and Jun N terminus kinase phosphorylation. Conclusion: We conclude that knockdown of ILK with siRNA effectively inhibited VEGF-induced retinal endothelial cell attachment, spreading, migration and tube formation. p38 MAPK and Akt are downstream signaling pathways of the ILK that regulated VEGF-induced retinal neovascularization. Targeting ILK may be a potentially useful therapeutic approach for treating ocular neovascularization. Copyright (c) 2012 S. Karger AG, Basel

语种英语
WOS记录号WOS:000312897400004
项目编号7092110 ; 2011CB510200
资助机构Beijing Natural Science Foundation ; National Basic Research Program of China (973 Program)
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52491
专题北京大学第二临床医学院_眼科
作者单位1.Shandong Prov Hosp, Dept Ophthalmol, Jinan, Peoples R China
2.Peking Univ, Dept Ophthalmol, Peoples Hosp, Key Lab Vis Loss & Restorat,Minist Educ, Beijing 100044, Peoples R China
3.China Acad Chinese Med Sci, Hosp Eye, Dept Ophthalmol, Beijing, Peoples R China
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GB/T 7714
Xie, Wankun,Zhao, Min,Zhou, Weiyan,et al. Targeting of Integrin-Linked Kinase with Small Interfering RNA Inhibits VEGF-Induced Angiogenesis in Retinal Endothelial Cells[J]. OPHTHALMIC RESEARCH,2013,49(3):139-149.
APA Xie, Wankun.,Zhao, Min.,Zhou, Weiyan.,Guo, Lili.,Huang, Lvzhen.,...&Li, Xiaoxin.(2013).Targeting of Integrin-Linked Kinase with Small Interfering RNA Inhibits VEGF-Induced Angiogenesis in Retinal Endothelial Cells.OPHTHALMIC RESEARCH,49(3),139-149.
MLA Xie, Wankun,et al."Targeting of Integrin-Linked Kinase with Small Interfering RNA Inhibits VEGF-Induced Angiogenesis in Retinal Endothelial Cells".OPHTHALMIC RESEARCH 49.3(2013):139-149.
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