IR@PKUHSC  > 北京大学第二临床医学院  > 泌尿外科
学科主题临床医学
CMTM5 is reduced in prostate cancer and inhibits cancer cell growth in vitro and in vivo
Xiao, Y.1,2; Yuan, Y.1,3; Zhang, Y.3; Li, J.1; Liu, Z.1; Zhang, X.1; Sheng, Z.1; Xu, T.1; Wang, X.1
关键词Cmtm5 Prostate Cancer Tm4sf Tumor Suppressor Gene Gene Therapy
刊名CLINICAL & TRANSLATIONAL ONCOLOGY
2015-06-01
DOI10.1007/s12094-014-1253-z
17期:6页:431-437
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]DOWN-REGULATION ; GENE ; SUPPRESSOR ; EXPRESSION ; CARCINOMA ; METHYLATION ; APOPTOSIS ; INVASION ; RECEPTOR ; IDENTIFICATION
英文摘要

A novel tumor suppressor CKLF-like MARVEL transmembrane domain-containing member 5 (CMTM5) is reduced or undetectable in many kinds of cancers and inhibits tumor cells′ malignant features. To explore its role in prostate cancer (PCa), we detected its expression patterns in prostate tissues and PCa cells, and determined its anti-proliferation functions in PCa cells in vitro and in vivo.

The expression of CMTM5 in prostate tissue microarray, specimens and cell lines was evaluated by immunohistochemistry and Western blot, respectively. After being transfected with CMTM5 adenovirus or vector, the proliferation and migration of DU145 cells were detected by MTT assay and transwell assay, respectively. Furthermore, the effects of CMTM5 on tumor growth were performed in nude mice xenograft in vivo.

We found CMTM5 was reduced in PCa tissues and cells compared with BPH tissues, and its expression in PCa tissues was related to the Gleason score. Moreover, after being transfected with adenovirus, ectopic expression of CMTM5-v1 in DU145 cells led to significant inhibition of cell proliferation and migration compared with the control, which may be attributed to decreased Akt activity. Finally, restoration of CMTM5 significantly suppressed tumor growth in vivo.

These results indicate that CMTM5 is down-regulated in PCa and exhibit tumor suppressor activities in androgen-independent PCa cells. Loss of CMTM5 protein may be contributed to the development of PCa and it is a potential therapeutic target for castration-resistant prostate cancer.

语种英语
WOS记录号WOS:000355340900002
项目编号81072099
资助机构National Natural Science Foundation of China (NSFC)
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52501
专题北京大学第二临床医学院_泌尿外科
北京大学基础医学院
北京大学第三临床医学院_麻醉科
北京大学第三临床医学院_运动医学研究所
作者单位1.Peking Univ, Peoples Hosp, Dept Urol, Beijing 100044, Peoples R China
2.Wenzhou Med Univ, Affiliated Hosp 1, Dept Urol, Wenzhou 325000, Peoples R China
3.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Dept Urol, Shenzhen 518020, Peoples R China
推荐引用方式
GB/T 7714
Xiao, Y.,Yuan, Y.,Zhang, Y.,et al. CMTM5 is reduced in prostate cancer and inhibits cancer cell growth in vitro and in vivo[J]. CLINICAL & TRANSLATIONAL ONCOLOGY,2015,17(6):431-437.
APA Xiao, Y..,Yuan, Y..,Zhang, Y..,Li, J..,Liu, Z..,...&Wang, X..(2015).CMTM5 is reduced in prostate cancer and inhibits cancer cell growth in vitro and in vivo.CLINICAL & TRANSLATIONAL ONCOLOGY,17(6),431-437.
MLA Xiao, Y.,et al."CMTM5 is reduced in prostate cancer and inhibits cancer cell growth in vitro and in vivo".CLINICAL & TRANSLATIONAL ONCOLOGY 17.6(2015):431-437.
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