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学科主题: 临床医学
题名:
CMTM5 is reduced in prostate cancer and inhibits cancer cell growth in vitro and in vivo
作者: Xiao, Y.1,2; Yuan, Y.1,3; Zhang, Y.3; Li, J.1; Liu, Z.1; Zhang, X.1; Sheng, Z.1; Xu, T.1; Wang, X.1
关键词: CMTM5 ; Prostate cancer ; TM4SF ; Tumor suppressor gene ; Gene therapy
刊名: CLINICAL & TRANSLATIONAL ONCOLOGY
发表日期: 2015-06-01
DOI: 10.1007/s12094-014-1253-z
卷: 17, 期:6, 页:431-437
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: DOWN-REGULATION ; GENE ; SUPPRESSOR ; EXPRESSION ; CARCINOMA ; METHYLATION ; APOPTOSIS ; INVASION ; RECEPTOR ; IDENTIFICATION
英文摘要:

A novel tumor suppressor CKLF-like MARVEL transmembrane domain-containing member 5 (CMTM5) is reduced or undetectable in many kinds of cancers and inhibits tumor cells′ malignant features. To explore its role in prostate cancer (PCa), we detected its expression patterns in prostate tissues and PCa cells, and determined its anti-proliferation functions in PCa cells in vitro and in vivo.

The expression of CMTM5 in prostate tissue microarray, specimens and cell lines was evaluated by immunohistochemistry and Western blot, respectively. After being transfected with CMTM5 adenovirus or vector, the proliferation and migration of DU145 cells were detected by MTT assay and transwell assay, respectively. Furthermore, the effects of CMTM5 on tumor growth were performed in nude mice xenograft in vivo.

We found CMTM5 was reduced in PCa tissues and cells compared with BPH tissues, and its expression in PCa tissues was related to the Gleason score. Moreover, after being transfected with adenovirus, ectopic expression of CMTM5-v1 in DU145 cells led to significant inhibition of cell proliferation and migration compared with the control, which may be attributed to decreased Akt activity. Finally, restoration of CMTM5 significantly suppressed tumor growth in vivo.

These results indicate that CMTM5 is down-regulated in PCa and exhibit tumor suppressor activities in androgen-independent PCa cells. Loss of CMTM5 protein may be contributed to the development of PCa and it is a potential therapeutic target for castration-resistant prostate cancer.

语种: 英语
所属项目编号: 81072099
项目资助者: National Natural Science Foundation of China (NSFC)
WOS记录号: WOS:000355340900002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52501
Appears in Collections:北京大学第二临床医学院_泌尿外科_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Dept Urol, Beijing 100044, Peoples R China
2.Wenzhou Med Univ, Affiliated Hosp 1, Dept Urol, Wenzhou 325000, Peoples R China
3.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Dept Urol, Shenzhen 518020, Peoples R China

Recommended Citation:
Xiao, Y.,Yuan, Y.,Zhang, Y.,et al. CMTM5 is reduced in prostate cancer and inhibits cancer cell growth in vitro and in vivo[J]. CLINICAL & TRANSLATIONAL ONCOLOGY,2015,17(6):431-437.
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