|Detection of Human Telomerase RNA Gene in Cervical Cancer and Precancerous Lesions Comparison With Cytological and Human Papillomavirus DNA Test Findings|
|Li, Ying2; Ye, Feng1,2; Lue, Wei-Guo1; Zeng, Wen-Jie2; Wei, Li-Hui3; Xie, Xing1|
|关键词||Cervical Cancer Cin Screening Method Human Telomerase Rna Gene (Hterc)|
|刊名||INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER|
|WOS标题词||Science & Technology|
|类目[WOS]||Oncology ; Obstetrics & Gynecology|
|研究领域[WOS]||Oncology ; Obstetrics & Gynecology|
|关键词[WOS]||PAPANICOLAOU SMEARS ; FREQUENT GAIN ; AMPLIFICATION ; TERC ; 3Q26 ; INTEGRATION ; EXPRESSION ; CARCINOMA ; DYSPLASIA ; EVENTS|
Objectives: The aims of this study were to compare the findings of fluorescence in situ hybridization (FISH) detection of human telomerase RNA gene (hTERC) amplification with that of cytological and human papillomavirus (HPV) DNA tests and explore the possibility to improve the accuracy of the diagnoses of high-grade cervical intraepithelial neoplasia (CIN) and cervical cancer.
Methods: A total of 201 specimens of liquid-based thin-layer cytological examination findings were collected and detected by HPV DNA test and hTERC detected by FISH. All women underwent colposcopy and histological examination of biopsy specimen if needed. The 3 screening methods were compared based on histological examination of colposcopic biopsies.
Results: The amplification of hTERC showed 6.06% in normal or inflammation cases, 10.00% in CIN 1, 66.67% in CIN 2, 72.50% in CIN 3, and 100.00% in carcinoma, with significant difference between the low-(<= CIN 1 or <= low-grade squamous intraepithelial lesion) and high-grade (>= CIN 2 or >= atypical squamous cells in which high-grade squamous intraepithelial lesion cannot be excluded) cervical lesions (P < 0.001). The hTERC amplification rate was consistent with abnormal rates of cytological and histological diagnoses. The detection of hTERC amplification had a much higher accuracy (87.56%) than the cytological (80.10%) and HPV DNA test (77.61%) findings.
Conclusions: Using FISH to detect the amplification of hTERC had a much higher specificity and accuracy for the diagnoses of high-grade CIN and cervical cancer than cytological and HPV DNA test findings, suggesting that this detection might be a useful and specific screening method in cervical cancer and precancerous lesions.
|项目编号||WKJ2007-3-001 ; WKJ2007-2-014 ; 200802109 ; 2006C13080|
|资助机构||Ministry of Health ; Special Research Projects of Health Industry ; Scientific and Technologic Grant of Zhejiang Province|
|作者单位||1.Zhejiang Univ, Womens Hosp, Sch Med, Hangzhou 310006, Zhejiang, Peoples R China|
2.Zhejiang Univ, Womens Reprod Hlth Lab Zhejiang Prov, Hangzhou 310006, Zhejiang, Peoples R China
3.Peking Univ, Peoples Hosp, Dept Gynecol, Beijing 100871, Peoples R China
|Li, Ying,Ye, Feng,Lue, Wei-Guo,et al. Detection of Human Telomerase RNA Gene in Cervical Cancer and Precancerous Lesions Comparison With Cytological and Human Papillomavirus DNA Test Findings[J]. INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER,2010,20(4):631-637.|
|APA||Li, Ying,Ye, Feng,Lue, Wei-Guo,Zeng, Wen-Jie,Wei, Li-Hui,&Xie, Xing.(2010).Detection of Human Telomerase RNA Gene in Cervical Cancer and Precancerous Lesions Comparison With Cytological and Human Papillomavirus DNA Test Findings.INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER,20(4),631-637.|
|MLA||Li, Ying,et al."Detection of Human Telomerase RNA Gene in Cervical Cancer and Precancerous Lesions Comparison With Cytological and Human Papillomavirus DNA Test Findings".INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER 20.4(2010):631-637.|