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IR@PKUHSC  > 北京大学药学院  > 药剂学系  > 期刊论文
学科主题: 药学
题名:
Methamphetamine regulation of sulfotransferase 1A1 and 2A1 expression in rat brain sections
作者: Zhou, Tianyan1,3; Huang, Chaoqun2; Chen, Yue2; Xu, Jiaojiao1; Shanbhag, Preeti Devaraya2; Chen, Guangping2
关键词: Sulfotransferase ; Methamphetamine ; Gene regulation ; Rat brain sections ; rSULT1A1 ; rSULT2A1
刊名: NEUROTOXICOLOGY
发表日期: 2013
DOI: 10.1016/j.neuro.2012.09.010
卷: 34, 页:212-218
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences ; Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]: Neurosciences & Neurology ; Pharmacology & Pharmacy ; Toxicology
关键词[WOS]: CYTOSOLIC SULFOTRANSFERASE ; DOPAMINERGIC SYSTEM ; LIVER CYTOCHROME-P450 ; PHENOL SULFOTRANSFERASE ; PARKINSONS-DISEASE ; GENE-TRANSCRIPTION ; ANIMAL-MODELS ; SULT1A3 ; SULFATION ; INHIBITORS
英文摘要:

Sulfotransferase catalyzed sulfation regulates the biological activities of various neurotransmitters/hormones and detoxifies xenobiotics. Rat sulfotransferase rSULT1A1 catalyzes the sulfation of neurotransmitters and xenobiotic phenolic compounds. rSULT2A1 catalyzes the sulfation of hydroxysteroids and xenobiotic alcoholic compounds. In this work, Western blot and real-time RT-PCR were used to investigate the effect of methamphetamine on rSULT1A1 and rSULT2A1 protein and mRNA expression in rat cerebellum, frontal cortex, hippocampus, and striatum. After 1-day treatment, significant induction of rSULT1A1 was observed only in the cerebellum; rSULT2A1 was induced significantly in the cerebellum, frontal cortex, and hippocampus. After 7 days of exposure, rSULT1A1 was induced in the cerebellum, frontal cortex, and hippocampus, while rSULT2A1 was induced significantly in all four regions. Western blot results agreed with the real-time RT-PCR results, suggesting that the induction occurred at the gene transcriptional level. Results indicate that rSULT1A1 and rSULT2A1 are expressed in rat frontal cortex, cerebellum, striatum, and hippocampus. rSULT1A1 and rSULT2A1 are inducible by methamphetamine in rat brain sections in a time dependable manner. rSULT2A1 is more inducible than rSULT1A1 by methamphetamine in rat brain sections. Induction activity of methamphetamine is in the order of cerebellum > frontal cortex, hippocampus > striatum. These results suggest that the physiological functions of rSULT1A1 and rSULT2A1 in different brain regions can be affected by methamphetamine. (C) 2012 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: GM078606 ; RSG-07-028-01-CNE ; 2006-35200-17137 ; HR05-015 ; 81072699
项目资助者: NIH ; American Cancer Society ; USDA ; Oklahoma Center for the Advancement of Science and Technology (OCAST) ; National Natural Science Foundation of China (NSFC)
WOS记录号: WOS:000314626100022
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52556
Appears in Collections:北京大学药学院_药剂学系_期刊论文

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作者单位: 1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China
2.Oklahoma State Univ, Dept Physiol Sci, Stillwater, OK 74078 USA
3.Peking Univ, Med Hlth Ctr, Sch Pharmaceut Sci, Dept Pharmaceut, Beijing 100871, Peoples R China

Recommended Citation:
Zhou, Tianyan,Huang, Chaoqun,Chen, Yue,et al. Methamphetamine regulation of sulfotransferase 1A1 and 2A1 expression in rat brain sections[J]. NEUROTOXICOLOGY,2013,34:212-218.
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