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学科主题: 临床医学
题名:
Integrated miRNA-mRNA Analysis Revealing the Potential Roles of miRNAs in Chordomas
作者: Long, Cheng1; Jiang, Liang1; Wei, Feng1; Ma, Chuan2; Zhou, Hua1; Yang, Shaomin3; Liu, Xiaoguang1; Liu, Zhongjun1
刊名: PLOS ONE
发表日期: 2013-06-24
DOI: 10.1371/journal.pone.0066676
卷: 8, 期:6
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: MICRORNA TARGETS ; NUCLEUS PULPOSUS ; GROWTH-FACTOR ; EXPRESSION ; CANCER ; TUMOR ; CARCINOMA ; NOTOCHORD ; CELLS ; MANAGEMENT
英文摘要:

Introduction: Emerging evidence suggests that microRNAs (miRNAs) are crucially involved in tumorigenesis and that paired expression profiles of miRNAs and mRNAs can be used to identify functional miRNA-target relationships with high precision. However, no studies have applied integrated analysis to miRNA and mRNA profiles in chordomas. The purpose of this study was to provide insights into the pathogenesis of chordomas by using this integrated analysis method.

Methods: Differentially expressed miRNAs and mRNAs of chordomas (n = 3) and notochord tissues (n = 3) were analyzed by using microarrays with hierarchical clustering analysis. Subsequently, the target genes of the differentially expressed miRNAs were predicted and overlapped with the differentially expressed mRNAs. Then, GO and pathway analyses were performed for the intersecting genes.

Results: The microarray analysis indicated that 33 miRNAs and 2,791 mRNAs were significantly dysregulated between the two groups. Among the 2,791 mRNAs, 911 overlapped with putative miRNA target genes. A pathway analysis showed that the MAPK pathway was consistently enriched in the chordoma tissue and that miR-149-3p, miR-663a, miR-1908, miR-2861 and miR-3185 likely play important roles in the regulation of MAPK pathways. Furthermore, the Notch signaling pathway and the loss of the calcification or ossification capacity of the notochord may also be involved in chordoma pathogenesis.

Conclusion: This study provides an integrated dataset of the miRNA and mRNA profiles in chordomas, and the results demonstrate that not only the MAPK pathway and its related miRNAs but also the Notch pathway may be involved in chordoma development. The occurrence of chordoma may be associated with dysfunctional calcification or ossification of the notochord.

语种: 英语
所属项目编号: WSB08-6-15
项目资助者: Ministry of Health Funded Key Projects for Clinical Medicine
WOS记录号: WOS:000321738400031
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52559
Appears in Collections:北京大学第三临床医学院_骨科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Orthoped, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 3, Spine Tumor Ctr, Beijing 100871, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100871, Peoples R China

Recommended Citation:
Long, Cheng,Jiang, Liang,Wei, Feng,et al. Integrated miRNA-mRNA Analysis Revealing the Potential Roles of miRNAs in Chordomas[J]. PLOS ONE,2013,8(6).
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