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学科主题临床医学
Detection of ADAMTS-4 Activity Using a Fluorogenic Peptide-Conjugated Au Nanoparticle Probe in Human Knee Synovial Fluid
Peng, Shi1; Zheng, Qiang2,3; Zhang, Xin1; Dai, Linghui1; Zhu, Jingxian1; Pi, Yanbin1; Hu, Xiaoging1; Cheng, Wenqing1; Zhou, Chunyan4; Sha, Yinlin2,3; Ao, Yingfang1
关键词Adamts-4 Gold Nanoparticles Osteoarthritis Fluorescent Probe Synovial Fluid
刊名ACS APPLIED MATERIALS & INTERFACES
2013-07-15
DOI10.1021/am400854z
5期:13页:6089-6096
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
研究领域[WOS]Science & Technology - Other Topics ; Materials Science
关键词[WOS]HUMAN ARTICULAR-CARTILAGE ; AGGRECAN ARGS FRAGMENTS ; GOLD NANOPARTICLES ; OSTEOARTHRITIC CARTILAGE ; IN-VITRO ; DEGRADATION ; CLEAVAGE ; ASSOCIATION ; PROGRESSION ; EXPRESSION
英文摘要

A disintegrin and metalloproteinase with thrombospondin motif-4 (ADAMTS-4) plays a pivotal role in degrading ag,grecan, which is an early event in cartilage degrading joint diseases such as osteoarthritis (OA). Detection of ADAMTS-4 activity could provide useful clinical information for early diagnosis of such diseases and disease-modifying therapy. Therefore, we developed a ADAMTS-4 detective fluorescent turn-on AuNP probe (ADAMTS-4-D-Au probe) by conjugating gold nanoparticles with a FITC-modified ADAMTS-4-specific peptide (DVQEFRGVTAVIR). When the ADAMTS-4-D-Au probe was incubated with ADAMTS-4, the fluorescence recovered and fluorescence intensity markedly increased in proportion to concentrations of ADAMTS-4 and the probe. A nearly 3-fold increase in fluorescent intensity in response to only 3.9 pM of ADAMTS-4 was detected, whereas almost no fluorescence recovery was observed when the probe was incubated with matrix metalloproteinase (MMP)-1, -3, and -13. These results indicate a relative high sensitivity and specificity of the probe. Moreover, ADAMTS-4-D-Au probe was used to detect ADAMTS-4 activity in synovial fluid from 11 knee surgery patients. A substantial increase in fluorescent intensity was observed in the acute joint injury group as compared to the chronic joint injury and end-stage OA groups, indicating that this simple and low-cost sensing system might serve as a new detection method for ADAMTS-4 activity in biological samples and in screens for inhibitors for ADAMTS-4-related joint diseases. Additionally, this probe could be a potential biomarker for early diagnosis of cartilage-degrading joint diseases.

语种英语
WOS记录号WOS:000321810000030
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52566
专题北京大学第三临床医学院_运动医学研究所
北京大学基础医学院
北京大学第三临床医学院_骨科
作者单位1.Peking Univ, Biomed X Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Inst Sports Med, Hosp 3, Beijing 100191, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Dept Biophys, Singlemol & Nanobiol Lab, Beijing 100191, Peoples R China
4.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
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Peng, Shi,Zheng, Qiang,Zhang, Xin,et al. Detection of ADAMTS-4 Activity Using a Fluorogenic Peptide-Conjugated Au Nanoparticle Probe in Human Knee Synovial Fluid[J]. ACS APPLIED MATERIALS & INTERFACES,2013,5(13):6089-6096.
APA Peng, Shi.,Zheng, Qiang.,Zhang, Xin.,Dai, Linghui.,Zhu, Jingxian.,...&Ao, Yingfang.(2013).Detection of ADAMTS-4 Activity Using a Fluorogenic Peptide-Conjugated Au Nanoparticle Probe in Human Knee Synovial Fluid.ACS APPLIED MATERIALS & INTERFACES,5(13),6089-6096.
MLA Peng, Shi,et al."Detection of ADAMTS-4 Activity Using a Fluorogenic Peptide-Conjugated Au Nanoparticle Probe in Human Knee Synovial Fluid".ACS APPLIED MATERIALS & INTERFACES 5.13(2013):6089-6096.
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