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学科主题: 基础医学
题名:
Induction of Pluripotency in Mouse Somatic Cells with Lineage Specifiers
作者: Shu, Jian1,3; Wu, Chen3,6; Wu, Yetao3,6; Li, Zhiyuan2,7; Shao, Sida1; Zhao, Wenhui1,3; Tang, Xing4; Yang, Huan3,6; Shen, Lijun1; Zuo, Xiaohan3,6; Yang, Weifeng1; Shi, Yan6; Chi, Xiaochun5; Zhang, Hongquan5; Gao, Ge4; Shu, Youmin8; Yuan, Kehu8; He, Weiwu8; Tang, Chao2,3; Zhao, Yang1,3; Deng, Hongkui1,3,6
刊名: CELL
发表日期: 2013-05-23
DOI: 10.1016/j.cell.2013.05.001
卷: 153, 期:5, 页:963-975
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: EMBRYONIC STEM-CELLS ; HUMAN FIBROBLASTS ; PRIMITIVE ENDODERM ; DIRECT CONVERSION ; GROUND-STATE ; SELF-RENEWAL ; DIFFERENTIATION ; GENERATION ; OCT4 ; EXPRESSION
英文摘要:

The reprogramming factors that induce pluripotency have been identified primarily from embryonic stem cell (ESC)-enriched, pluripotency-associated factors. Here, we report that, during mouse somatic cell reprogramming, pluripotency can be induced with lineage specifiers that are pluripotency rivals to suppress ESC identity, most of which are not enriched in ESCs. We found that OCT4 and SOX2, the core regulators of pluripotency, can be replaced by lineage specifiers that are involved in mesendo-dermal (ME) specification and in ectodermal (ECT) specification, respectively. OCT4 and its substitutes attenuated the elevated expression of a group of ECT genes, whereas SOX2 and its substitutes curtailed a group of ME genes during reprogramming. Surprisingly, the two counteracting lineage specifiers can synergistically induce pluripotency in the absence of both OCT4 and SOX2. Our study suggests a "seesaw model′′ in which a balance that is established using pluripotency factors and/or counteracting lineage specifiers can facilitate reprogramming.

语种: 英语
所属项目编号: 2012CB966401 ; 2009CB941101 ; 2010CB945204 ; 2009CB918500 ; 2011ZX09102-010-03 ; 90919031 ; 11021463 ; CMMI-0941355
项目资助者: National Basic Research Program of China (973 program) ; Key New Drug Creation and Manufacturing Program ; National Natural Science Foundation of China ; 111 project ; NSF
WOS记录号: WOS:000319456800006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52572
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.OriGene Technol, Rockville, MD 20850 USA
2.Peking Univ, Coll Life Sci, MOE Key Lab Cell Proliferat & Differentiat, Beijing 100871, Peoples R China
3.Peking Univ, Ctr Quantitat Biol, Beijing 100871, Peoples R China
4.Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China
5.Peking Univ, Ctr Bioinformat, State Key Lab Prot & Plant Gene Res, Coll Life Sci, Beijing 100871, Peoples R China
6.Peking Univ, Sch Basic Med Sci, Lab Stem Cells Dev & Reprod Med, Beijing 100191, Peoples R China
7.Peking Univ, Shenzhen Grad Sch, Sch Chem Biol & Biotechnol, Lab Chem Genom, Shenzhen 518055, Peoples R China
8.Univ Calif San Francisco, Biophys Grad Program, San Francisco, CA 94158 USA

Recommended Citation:
Shu, Jian,Wu, Chen,Wu, Yetao,et al. Induction of Pluripotency in Mouse Somatic Cells with Lineage Specifiers[J]. CELL,2013,153(5):963-975.
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