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学科主题基础医学
Induction of Pluripotency in Mouse Somatic Cells with Lineage Specifiers
Shu, Jian1,3; Wu, Chen3,6; Wu, Yetao3,6; Li, Zhiyuan2,7; Shao, Sida1; Zhao, Wenhui1,3; Tang, Xing4; Yang, Huan3,6; Shen, Lijun1; Zuo, Xiaohan3,6; Yang, Weifeng1; Shi, Yan6; Chi, Xiaochun5; Zhang, Hongquan5; Gao, Ge4; Shu, Youmin8; Yuan, Kehu8; He, Weiwu8; Tang, Chao2,3; Zhao, Yang1,3; Deng, Hongkui1,3,6
刊名CELL
2013-05-23
DOI10.1016/j.cell.2013.05.001
153期:5页:963-975
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
资助者National Basic Research Program of China (973 program) ; Key New Drug Creation and Manufacturing Program ; National Natural Science Foundation of China ; 111 project ; NSF ; National Basic Research Program of China (973 program) ; Key New Drug Creation and Manufacturing Program ; National Natural Science Foundation of China ; 111 project ; NSF
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]EMBRYONIC STEM-CELLS ; HUMAN FIBROBLASTS ; PRIMITIVE ENDODERM ; DIRECT CONVERSION ; GROUND-STATE ; SELF-RENEWAL ; DIFFERENTIATION ; GENERATION ; OCT4 ; EXPRESSION
英文摘要

The reprogramming factors that induce pluripotency have been identified primarily from embryonic stem cell (ESC)-enriched, pluripotency-associated factors. Here, we report that, during mouse somatic cell reprogramming, pluripotency can be induced with lineage specifiers that are pluripotency rivals to suppress ESC identity, most of which are not enriched in ESCs. We found that OCT4 and SOX2, the core regulators of pluripotency, can be replaced by lineage specifiers that are involved in mesendo-dermal (ME) specification and in ectodermal (ECT) specification, respectively. OCT4 and its substitutes attenuated the elevated expression of a group of ECT genes, whereas SOX2 and its substitutes curtailed a group of ME genes during reprogramming. Surprisingly, the two counteracting lineage specifiers can synergistically induce pluripotency in the absence of both OCT4 and SOX2. Our study suggests a "seesaw model′′ in which a balance that is established using pluripotency factors and/or counteracting lineage specifiers can facilitate reprogramming.

语种英语
所属项目编号2012CB966401 ; 2009CB941101 ; 2010CB945204 ; 2009CB918500 ; 2011ZX09102-010-03 ; 90919031 ; 11021463 ; CMMI-0941355
资助者National Basic Research Program of China (973 program) ; Key New Drug Creation and Manufacturing Program ; National Natural Science Foundation of China ; 111 project ; NSF ; National Basic Research Program of China (973 program) ; Key New Drug Creation and Manufacturing Program ; National Natural Science Foundation of China ; 111 project ; NSF
WOS记录号WOS:000319456800006
引用统计
被引频次:139[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52572
专题基础医学院
作者单位1.OriGene Technol, Rockville, MD 20850 USA
2.Peking Univ, Coll Life Sci, MOE Key Lab Cell Proliferat & Differentiat, Beijing 100871, Peoples R China
3.Peking Univ, Ctr Quantitat Biol, Beijing 100871, Peoples R China
4.Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China
5.Peking Univ, Ctr Bioinformat, State Key Lab Prot & Plant Gene Res, Coll Life Sci, Beijing 100871, Peoples R China
6.Peking Univ, Sch Basic Med Sci, Lab Stem Cells Dev & Reprod Med, Beijing 100191, Peoples R China
7.Peking Univ, Shenzhen Grad Sch, Sch Chem Biol & Biotechnol, Lab Chem Genom, Shenzhen 518055, Peoples R China
8.Univ Calif San Francisco, Biophys Grad Program, San Francisco, CA 94158 USA
推荐引用方式
GB/T 7714
Shu, Jian,Wu, Chen,Wu, Yetao,et al. Induction of Pluripotency in Mouse Somatic Cells with Lineage Specifiers[J]. CELL,2013,153(5):963-975.
APA Shu, Jian.,Wu, Chen.,Wu, Yetao.,Li, Zhiyuan.,Shao, Sida.,...&Deng, Hongkui.(2013).Induction of Pluripotency in Mouse Somatic Cells with Lineage Specifiers.CELL,153(5),963-975.
MLA Shu, Jian,et al."Induction of Pluripotency in Mouse Somatic Cells with Lineage Specifiers".CELL 153.5(2013):963-975.
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