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学科主题: 药学
题名:
The transport mechanisms of polymer nanoparticles in Caco-2 epithelial cells
作者: He, Bing; Lin, Ping; Jia, Zengrong; Du, Wenwen; Qu, Wei; Yuan, Lan; Dai, Wenbing; Zhang, Hua; Wang, Xueqing; Wang, Jiancheng; Zhang, Xuan; Zhang, Qiang
关键词: Polymer nanoparticles ; Caco-2 epithelial cells ; Endocytosis ; Intracellular trafficking ; Exocytosis ; Transcytosis
刊名: BIOMATERIALS
发表日期: 2013-08-01
DOI: 10.1016/j.biomaterials.2013.04.053
卷: 34, 期:25, 页:6082-6098
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]: Engineering ; Materials Science
关键词[WOS]: MDCK CELLS ; TRANSCELLULAR TRANSPORT ; ENDOCYTOSIS ; TRANSCYTOSIS ; PATHWAY ; MEMBRANE ; DELIVERY ; CYCLOOXYGENASE-2 ; INTERNALIZATION ; COLOCALIZATION
英文摘要:

As the primary physiological barrier, intestinal epithelial cells regulate the transportation of oral therapeutic agents including nanomedicines which significantly improves the bioavailability of many drugs. However, currently there seems in the lack of comprehensive understanding on nanoparticle transport in intestinal epithelial cells as well as the mechanisms related. So, in an attempt to illustrate the profile of nanoparticle transport in intestinal epithelial cells, Caco-2 cells and polymer nanoparticles (PNs) were used as the models to explore the whole transport process including endocytosis, intracellular trafficking, exocytosis and transcytosis. Via various techniques, the transport pathways of PNs in Caco-2 cells and their mechanisms were clarified. Firstly, the transport was characterized by its non-specificity. The co-mediation of clathrin, lipid raft/caveolae and macropinocytosis as well as the co-involvement of different proteins like actins, protein tyrosine kinase (PTK) and cyclooxygenase (COX) were found in the endocytosis of PNs. The endocytosed PNs could transport to apical early endosome (AEE) and then from AEE to lysososmes via AEE/late endosome (LE)/lysosome pathway, as well as to recycling endosome compartment (REC) or endoplasmic reticulum (ER) through AEE/REC and AEE/ER pathways, respectively. Both ER/Golgi and Golgi/REC/plasma membrane (PM) pathways were involved in the exocytosis of PNs. The transcytosis of PNs across the cell monolayer was very low with a ratio less than 0.5%, due to complicated reasons. Secondly, the transport was evidenced by its partial energy-dependency. Beside the energy-dependent transport mediated by some proteins, quantitative study demonstrated the obvious internalization as well as surface binding of PNs at both 37 degrees C and 4 degrees C, but significantly higher at 37 degrees C. Interestingly, the consistency between surface binding and internalization at each temperature was found, suggesting that cell binding was the precondition and key step for the following endocytosis. The involvement of both energy dependent and independent mechanism was also observed in the exocytosis and transcytosis process of PNs. Finally, there were opposite mechanisms found between the exocytosis and endocytosis of PNs, including the regulation role of lipid raft/caveolae, COX and Golgi complex, which also contributed to the fact of "easy entry and hard across" for PNs. Overall, this study depicts a clear picture of nanoparticle transport in Caco-2 epithelial cells characterized by non-specificity, partial energy-dependency and low transcytosis. (C) 2013 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 2009CB930300 ; 81130059
项目资助者: National Basic Research Program of China ; National Natural Science Foundation of China
WOS记录号: WOS:000320906100009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52574
Appears in Collections:北京大学药学院_期刊论文

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作者单位: Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

Recommended Citation:
He, Bing,Lin, Ping,Jia, Zengrong,et al. The transport mechanisms of polymer nanoparticles in Caco-2 epithelial cells[J]. BIOMATERIALS,2013,34(25):6082-6098.
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