北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学口腔医学院  > 口腔病理科  > 期刊论文
学科主题: 口腔医学
题名:
Moesin regulates the motility of oral cancer cells via MT1-MMP and E-cadherin/p120-catenin adhesion complex
作者: Li, Yao-yin; Zhou, Chuan-Xiang; Gao, Yan
关键词: Moesin ; E-cadherin/p120-catenin ; MT1-MMP ; Oral squamous cell carcinoma ; Cell motility
刊名: ORAL ONCOLOGY
发表日期: 2015-10-01
DOI: 10.1016/j.oraloncology.2015.07.003
卷: 51, 期:10, 页:935-943
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Oncology ; Dentistry, Oral Surgery & Medicine
关键词[WOS]: E-CADHERIN ; MATRIX METALLOPROTEINASES ; TUMOR INVASION ; BREAST-CANCER ; ERM PROTEINS ; EZRIN ; METASTASIS ; CARCINOMA ; FAMILY ; PROGRESSION
英文摘要:

Objective: The present study aimed to clarify the role of Moesin in oral squamous cell carcinoma (OSCC) progression, especially in regulation of cell motility.

Materials and Methods: Immunohistochemistry and western blotting were used to investigate the expression of Moesin, E-cadherin, p120-catenin and MT1-MMP in normal epithelia, dysplasia and OSCCs. Then, Moesin was knockdown by siRNA in OSCC cell lines, WSU-HN6 and CAL27, and the biological role of Moesin in cell adhesion and motility was evaluated by transwell system, cell spreading and aggregation assays. The interactions between Moesin, MT1-MMP and E-cadherin/p120-catenin complex were determined by co-immunoprecipitation and immunofluorescence.

Results: Moesin expression was found decreased in the membrane and increased in cytoplasm during the malignant transformation of oral epithelia, and cytoplasmic overexpression of Moesin correlated with nodal metastasis and poor prognosis of OSCCs. Furthermore, Moesin-silencing induced an increased cell-cell adhesion but decreased invasiveness, which was subsequently demonstrated might due to Moesin-mediated E-cadherin and p120-catenin interaction. Meantime, Moesin-silencing significantly down-regulated MT1-MMP expression, accompanied by reduced cell motility and impaired filopodia formation, which was also observed when MT1-MMP knockdown by RNAi or tissue inhibitor (TIMP2), indicating the involvement of MT1-MMP in Moesin-mediated cell motility. Finally, the relationship between Moesin, E-cadherin and MT1-MMP was confirmed in OSCC tissue samples.

Conclusion: Taken together, our results indicate Moesin may regulate cell motility through its interactions with MT1-MMP and E-cadherin/p120-catenin adhesion complex and cytoplasmic expression of Moesin correlates with nodal metastasis and poor prognosis of OSCCs, indicating Moesin may be a potential candidate for targeted gene therapy for OSCCs. (c) 2015 Elsevier Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000361240200010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52578
Appears in Collections:北京大学口腔医学院_口腔病理科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Sch & Hosp Stomatol, Dept Oral Pathol, Beijing 100081, Peoples R China

Recommended Citation:
Li, Yao-yin,Zhou, Chuan-Xiang,Gao, Yan. Moesin regulates the motility of oral cancer cells via MT1-MMP and E-cadherin/p120-catenin adhesion complex[J]. ORAL ONCOLOGY,2015,51(10):935-943.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li, Yao-yin]'s Articles
[Zhou, Chuan-Xiang]'s Articles
[Gao, Yan]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li, Yao-yin]‘s Articles
[Zhou, Chuan-Xiang]‘s Articles
[Gao, Yan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  北京大学医学部 - Feedback
Powered by CSpace