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学科主题: 基础医学
题名:
Heterogeneity in predisposition of hepatic cells to be induced into pancreatic endocrine cells by PDX-1
作者: Lu, Shun1; Wang, Wei-Ping1; Wang, Xiao-Fei1; Zheng, Zong-Mei2; Chen, Ping1; Ma, Kang-Tao1; Zhou, Chun-Yan1
关键词: PDX-1 ; HepG2 ; Insulin ; Transgene ; Diabetes
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2005-04-21
卷: 11, 期:15, 页:2277-2282
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: INSULIN GENE-TRANSCRIPTION ; EMBRYONIC STEM-CELLS ; IN-VITRO ; HOMEOBOX FACTOR ; BETA-CELLS ; LIVER ; EXPRESSION ; THERAPY ; MICE ; DIFFERENTIATION
英文摘要:

AIM: The role of Pancreatic and Duodenal Homeobox-1 (PDX-1) as a major regulator of pancreatic development determines the function and phenotype of beta cell. In this study, potential plasticity of liver cells into pancreatic endocrine cells induced by PDX-1 was evaluated.

METHODS: Human hepatoma cell line HepG2 was stably transfected with mammalian expression plasmid pcDNA3-PDX encoding human PDX-1 gene. Ectopic expression of PDX-1 and insulin were detected by RT-PCR, Western blot and/or immunostaining. PDX-1(+) HepG2 cells were transplanted under renal capsule of STZ-induced diabetic nude mice (n = 16) to examine the inducing effect in vivo.

RESULTS: Exogenous PDX-1 transgene was proved to express effectively in HepG2 cell at both mRNA and protein levels. The expression of endogenous insulin and some beta cell-specific differentiation markers and transcription factors were not induced in PDX-1(+) HepG2 cells. When transplanted under renal capsule of STZ-induced diabetic nude mice, PDX-1(+) HepG2 cells did not generate insulin-producing cells. These data indicated that stable transfected PDX-1 could not convert hepatoma cell line HepG2 to pancreatic cells in vitro or in vivo. Mature hepatocytes might need much more complicated or rigorous conditions to be shifted to insulin-producing cells.

CONCLUSION: The expression of exogenous PDX-1 is not sufficient to induce relatively mature hepatocytes differentiating into insulin-producing cells. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 30240087 ; 2003AA205090
项目资助者: National Natural Science Foundation of China ; National High Technology Research and Development Program of China
WOS记录号: WOS:000208102700013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52579
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ Stem Cell Res Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Dept Biochem & Mol Biol, Sch Basic Med Sci, Beijing 100083, Peoples R China

Recommended Citation:
Lu, Shun,Wang, Wei-Ping,Wang, Xiao-Fei,et al. Heterogeneity in predisposition of hepatic cells to be induced into pancreatic endocrine cells by PDX-1[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2005,11(15):2277-2282.
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