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|Heterogeneity in predisposition of hepatic cells to be induced into pancreatic endocrine cells by PDX-1|
|Lu, Shun1; Wang, Wei-Ping1; Wang, Xiao-Fei1; Zheng, Zong-Mei2; Chen, Ping1; Ma, Kang-Tao1; Zhou, Chun-Yan1|
|关键词||Pdx-1 Hepg2 Insulin Transgene Diabetes|
|刊名||WORLD JOURNAL OF GASTROENTEROLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Gastroenterology & Hepatology|
|研究领域[WOS]||Gastroenterology & Hepatology|
|关键词[WOS]||INSULIN GENE-TRANSCRIPTION ; EMBRYONIC STEM-CELLS ; IN-VITRO ; HOMEOBOX FACTOR ; BETA-CELLS ; LIVER ; EXPRESSION ; THERAPY ; MICE ; DIFFERENTIATION|
AIM: The role of Pancreatic and Duodenal Homeobox-1 (PDX-1) as a major regulator of pancreatic development determines the function and phenotype of beta cell. In this study, potential plasticity of liver cells into pancreatic endocrine cells induced by PDX-1 was evaluated.
METHODS: Human hepatoma cell line HepG2 was stably transfected with mammalian expression plasmid pcDNA3-PDX encoding human PDX-1 gene. Ectopic expression of PDX-1 and insulin were detected by RT-PCR, Western blot and/or immunostaining. PDX-1(+) HepG2 cells were transplanted under renal capsule of STZ-induced diabetic nude mice (n = 16) to examine the inducing effect in vivo.
RESULTS: Exogenous PDX-1 transgene was proved to express effectively in HepG2 cell at both mRNA and protein levels. The expression of endogenous insulin and some beta cell-specific differentiation markers and transcription factors were not induced in PDX-1(+) HepG2 cells. When transplanted under renal capsule of STZ-induced diabetic nude mice, PDX-1(+) HepG2 cells did not generate insulin-producing cells. These data indicated that stable transfected PDX-1 could not convert hepatoma cell line HepG2 to pancreatic cells in vitro or in vivo. Mature hepatocytes might need much more complicated or rigorous conditions to be shifted to insulin-producing cells.
CONCLUSION: The expression of exogenous PDX-1 is not sufficient to induce relatively mature hepatocytes differentiating into insulin-producing cells. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.
|项目编号||30240087 ; 2003AA205090|
|资助机构||National Natural Science Foundation of China ; National High Technology Research and Development Program of China|
|作者单位||1.Peking Univ Stem Cell Res Ctr, Beijing 100083, Peoples R China|
2.Peking Univ, Dept Biochem & Mol Biol, Sch Basic Med Sci, Beijing 100083, Peoples R China
|Lu, Shun,Wang, Wei-Ping,Wang, Xiao-Fei,et al. Heterogeneity in predisposition of hepatic cells to be induced into pancreatic endocrine cells by PDX-1[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2005,11(15):2277-2282.|
|APA||Lu, Shun.,Wang, Wei-Ping.,Wang, Xiao-Fei.,Zheng, Zong-Mei.,Chen, Ping.,...&Zhou, Chun-Yan.(2005).Heterogeneity in predisposition of hepatic cells to be induced into pancreatic endocrine cells by PDX-1.WORLD JOURNAL OF GASTROENTEROLOGY,11(15),2277-2282.|
|MLA||Lu, Shun,et al."Heterogeneity in predisposition of hepatic cells to be induced into pancreatic endocrine cells by PDX-1".WORLD JOURNAL OF GASTROENTEROLOGY 11.15(2005):2277-2282.|
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