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MicroRNA-509-3p inhibits cancer cell proliferation and migration by targeting the mitogen-activated protein kinase kinase kinase 8 oncogene in renal cell carcinoma
Su, Zhengming1,2,3; Chen, Duqun1,4; Zhang, Enpu1,2; Li, Yifan1,4; Yu, Zuhu1,4; Shi, Min1,2; Jiang, Zhimao1,2; Ni, Liangchao1,2; Yang, Shangqi1,2; Gui, Yaoting1,2; Ye, Jiongxian1,2,3; Lai, Yongqing1,2,3
关键词Renal Cell Carcinoma Microrna-509-3p Mitogen-activated Protein Kinase Kinase Kinase 8
刊名MOLECULAR MEDICINE REPORTS
2015-07-01
DOI10.3892/mmr.2015.3498
12期:1页:1535-1543
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Medicine, Research & Experimental
研究领域[WOS]Oncology ; Research & Experimental Medicine
关键词[WOS]MAP KINASE ; EXPRESSION ; INVASION ; OVEREXPRESSION ; IDENTIFICATION ; METASTASIS ; APOPTOSIS ; MIR-7
英文摘要

microRNAs (miRNAs; miR) are a class of small non-coding RNA molecules, which are involved in the pathogenesis of human diseases through the negative regulation of gene expression. Previous studies have demonstrated that miR-509-3p is a novel miRNA associated with cell proliferation and migration in 786-O renal cell carcinoma (RCC) cells. However, the mechanism of action of miR-509-3p in RCC remains to be elucidated. The present study aimed to examine the functional role and mechanism of miR-509-3p in the development of RCC. The results demonstrated that the expression levels of miR-509-3p were downregulated in the 786-O and ACHN RCC cell lines compared with the normal tissues of 10 patients with RCC, as determined by reverse transcription-quantitative polymerase chain reaction. The mRNA expression levels of mitogen-activated protein kinase kinase kinase 8 (MAP3K8) were upregulated in the RCC cell lines. Functional investigations demonstrated that the overexpression of miR-509-3p inhibited the migration and proliferation of the RCC cells, as determined by wound scratch and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. Luciferase reporter assays revealed that the overexpression of miR-509-3p reduced the transcriptional activity of MAP3K8. Furthermore, the present study demonstrated that the ectopic transfection of miR-509-3p led to a significant reduction in the mRNA and protein expression levels of MAP3K8 in the RCC cells. Finally, knockdown of MAP3K8 inhibited the migration and proliferation of the RCC cells. Therefore, the results of the present study demonstrated that the miR-509-3p RCC suppressor was a significant regulator of the MAP3K8 oncogene, suggesting that it may have a potential therapeutic role in the treatment of RCC.

语种英语
WOS记录号WOS:000358134400211
项目编号81101922 ; A2012584 ; A2013606 ; CYJ20130402114702124
资助机构National Natural Science Foundation of China ; Medical Scientific Research Foundation of Guangdong Province of China ; Science and Technology Development Fund Project of Shenzhen ; fund of the Guangdong Key Medical Subject
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52592
专题北京大学深圳医院_泌尿外科
北京大学第一临床医学院_泌尿外科
作者单位1.Peking Univ, Shenzhen Hosp, Dept Urol, Shenzhen 518036, Guangdong, Peoples R China
2.Peking Univ, Shenzhen Hosp, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Inst Urol,PKU HKUST Med Ctr, Shenzhen 518036, Guangdong, Peoples R China
3.Shantou Univ, Coll Med, Dept Urol, Shantou 515041, Guangdong, Peoples R China
4.Anhui Med Univ, Dept Urol, Hefei 230032, Anhui, Peoples R China
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GB/T 7714
Su, Zhengming,Chen, Duqun,Zhang, Enpu,et al. MicroRNA-509-3p inhibits cancer cell proliferation and migration by targeting the mitogen-activated protein kinase kinase kinase 8 oncogene in renal cell carcinoma[J]. MOLECULAR MEDICINE REPORTS,2015,12(1):1535-1543.
APA Su, Zhengming.,Chen, Duqun.,Zhang, Enpu.,Li, Yifan.,Yu, Zuhu.,...&Lai, Yongqing.(2015).MicroRNA-509-3p inhibits cancer cell proliferation and migration by targeting the mitogen-activated protein kinase kinase kinase 8 oncogene in renal cell carcinoma.MOLECULAR MEDICINE REPORTS,12(1),1535-1543.
MLA Su, Zhengming,et al."MicroRNA-509-3p inhibits cancer cell proliferation and migration by targeting the mitogen-activated protein kinase kinase kinase 8 oncogene in renal cell carcinoma".MOLECULAR MEDICINE REPORTS 12.1(2015):1535-1543.
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