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学科主题: 临床医学
题名:
MicroRNA-509-3p inhibits cancer cell proliferation and migration by targeting the mitogen-activated protein kinase kinase kinase 8 oncogene in renal cell carcinoma
作者: Su, Zhengming1,2,3; Chen, Duqun1,4; Zhang, Enpu1,2; Li, Yifan1,4; Yu, Zuhu1,4; Shi, Min1,2; Jiang, Zhimao1,2; Ni, Liangchao1,2; Yang, Shangqi1,2; Gui, Yaoting1,2; Ye, Jiongxian1,2,3; Lai, Yongqing1,2,3
关键词: renal cell carcinoma ; microRNA-509-3p ; mitogen-activated protein kinase kinase kinase 8
刊名: MOLECULAR MEDICINE REPORTS
发表日期: 2015-07-01
DOI: 10.3892/mmr.2015.3498
卷: 12, 期:1, 页:1535-1543
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Medicine, Research & Experimental
研究领域[WOS]: Oncology ; Research & Experimental Medicine
关键词[WOS]: MAP KINASE ; EXPRESSION ; INVASION ; OVEREXPRESSION ; IDENTIFICATION ; METASTASIS ; APOPTOSIS ; MIR-7
英文摘要:

microRNAs (miRNAs; miR) are a class of small non-coding RNA molecules, which are involved in the pathogenesis of human diseases through the negative regulation of gene expression. Previous studies have demonstrated that miR-509-3p is a novel miRNA associated with cell proliferation and migration in 786-O renal cell carcinoma (RCC) cells. However, the mechanism of action of miR-509-3p in RCC remains to be elucidated. The present study aimed to examine the functional role and mechanism of miR-509-3p in the development of RCC. The results demonstrated that the expression levels of miR-509-3p were downregulated in the 786-O and ACHN RCC cell lines compared with the normal tissues of 10 patients with RCC, as determined by reverse transcription-quantitative polymerase chain reaction. The mRNA expression levels of mitogen-activated protein kinase kinase kinase 8 (MAP3K8) were upregulated in the RCC cell lines. Functional investigations demonstrated that the overexpression of miR-509-3p inhibited the migration and proliferation of the RCC cells, as determined by wound scratch and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. Luciferase reporter assays revealed that the overexpression of miR-509-3p reduced the transcriptional activity of MAP3K8. Furthermore, the present study demonstrated that the ectopic transfection of miR-509-3p led to a significant reduction in the mRNA and protein expression levels of MAP3K8 in the RCC cells. Finally, knockdown of MAP3K8 inhibited the migration and proliferation of the RCC cells. Therefore, the results of the present study demonstrated that the miR-509-3p RCC suppressor was a significant regulator of the MAP3K8 oncogene, suggesting that it may have a potential therapeutic role in the treatment of RCC.

语种: 英语
所属项目编号: 81101922 ; A2012584 ; A2013606 ; CYJ20130402114702124
项目资助者: National Natural Science Foundation of China ; Medical Scientific Research Foundation of Guangdong Province of China ; Science and Technology Development Fund Project of Shenzhen ; fund of the Guangdong Key Medical Subject
WOS记录号: WOS:000358134400211
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52592
Appears in Collections:北京大学深圳医院_泌尿外科_期刊论文

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作者单位: 1.Peking Univ, Shenzhen Hosp, Dept Urol, Shenzhen 518036, Guangdong, Peoples R China
2.Peking Univ, Shenzhen Hosp, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Inst Urol,PKU HKUST Med Ctr, Shenzhen 518036, Guangdong, Peoples R China
3.Shantou Univ, Coll Med, Dept Urol, Shantou 515041, Guangdong, Peoples R China
4.Anhui Med Univ, Dept Urol, Hefei 230032, Anhui, Peoples R China

Recommended Citation:
Su, Zhengming,Chen, Duqun,Zhang, Enpu,et al. MicroRNA-509-3p inhibits cancer cell proliferation and migration by targeting the mitogen-activated protein kinase kinase kinase 8 oncogene in renal cell carcinoma[J]. MOLECULAR MEDICINE REPORTS,2015,12(1):1535-1543.
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