北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 心血管所  > 期刊论文
学科主题: 基础医学
题名:
Endogenous aldosterone is involved in vascular calcification in rat
作者: Wu, Sheng-Ying1,2; Yu, Yan-Rong1,3; Cai, Yan1,2; Jia, Li-Xin4; Wang, Xiong2; Xiao, Chuan-Shi4; Tang, Chao-Shu1,2; Qi, Yong-Fen1
关键词: aldosterone ; blood vessel ; calcification ; fibrosis ; rat
刊名: EXPERIMENTAL BIOLOGY AND MEDICINE
发表日期: 2012
DOI: 10.1258/ebm.2011.011175
卷: 237, 期:1, 页:31-37
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental
研究领域[WOS]: Research & Experimental Medicine
关键词[WOS]: SMOOTH-MUSCLE-CELLS ; ANGIOTENSIN-II ; MINERALOCORTICOID RECEPTOR ; CARDIOVASCULAR-SYSTEM ; OXIDATIVE STRESS ; MECHANISMS ; ADRENOMEDULLIN ; CALCIUM ; PATHWAY ; TARGET
英文摘要:

Aldosterone (Aldo) is an important active hormone in the renin-angiotensin-aldosterone system and plays a vital role in the development of hypertension, heart failure and other cardiovascular diseases. We aimed to explore the role of endogenous Aldo in aortic calcification in rats. We induced arterial calcification in rats by intramuscular administration of vitamin D-3 plus oral nicotine (VDN) and determined calcium content, Ca-45(2+) accumulation and activity of alkaline phosphatase (ALP). The mRNA level of osteopontin (OPN) was measured by semi-quantitative reverse transcriptase polymerase chain reaction. Deposition of collagen in the aorta wall was measured by Sirius red staining. The content of angiotensin II (Ang II) and Aldo in plasma and myocardial and vascular tissue was determined by radioimmunoassay. In rats with VDN treatment, von Kossa staining showed calcification in vascular smooth muscle cells and extracellular matrix, and the content of calcium in calcified arteries was 5.8-fold of that in control arteries (P < 0.01). The accumulation of Ca-45(2+) and activity of ALP in calcified aortic tissue was three- and 2.5-fold, respectively, that in control tissue (P < 0.01). The mRNA expression of OPN was significantly higher, by 58%, in calcified than control tissue (P < 0.01). Vascular fibrosis was greater in rats with calcified tissue than in control rats. The level of Ang II and Aldo was 58% and 80% higher, respectively, in calcified than control tissue (both P < 0.01). The changes could be significantly improved by treatment with captopril, an angiotensin-converting enzyme inhibitor, and the Aldo receptor antagonist spironolactone. These results suggest that Aldo is an endogenous bioactive factor involved in vascular calcification.

语种: 英语
所属项目编号: 30770869 ; 30871013 ; 81070090
项目资助者: National Natural Science Foundation of China ; Beijing Municipal Commission of Education
WOS记录号: WOS:000299865600005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52593
Appears in Collections:基础医学院_心血管所_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
ebm%2E2011%2E011175.pdf(354KB)期刊论文作者接受稿限制开放 联系获取全文

作者单位: 1.Peking Univ, Hlth Sci Ctr, Key Lab Mol Cardiovasc Sci, Minist Educ, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100191, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Med Parasitol, Sch Basic Med Sci, Beijing 100191, Peoples R China
4.Shanxi Med Univ, Dept Cardiol, Hosp 2, Taiyuan 030001, Shanxi, Peoples R China

Recommended Citation:
Wu, Sheng-Ying,Yu, Yan-Rong,Cai, Yan,et al. Endogenous aldosterone is involved in vascular calcification in rat[J]. EXPERIMENTAL BIOLOGY AND MEDICINE,2012,237(1):31-37.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wu, Sheng-Ying]'s Articles
[Yu, Yan-Rong]'s Articles
[Cai, Yan]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wu, Sheng-Ying]‘s Articles
[Yu, Yan-Rong]‘s Articles
[Cai, Yan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace