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Comparative analysis of two species of Asari Radix et Rhizoma by electronic nose, headspace GC-MS and chemometrics
Li, Chen; Xu, Feng; Cao, Chen; Shang, Ming-Ying; Zhang, Cui-Ying; Yu, Jie; Liu, Guang-Xue; Wang, Xuan; Cai, Shao-Qing
关键词Asari Radix Et Rhizoma Electronic Nose Headspace Gc-ms Chemometrics
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
2013-11-01
DOI10.1016/j.jpba.2013.07.034
85页:231-238
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]ESSENTIAL OILS ; QUALITY-CONTROL ; DISCRIMINATION
英文摘要

Traditional Chinese medicines (TCM) can be identified by experts according to their odors. However, the identification of these medicines is subjective and requires long-term experience. In this paper, electronic nose, headspace gas chromatography-mass spectrometry (GC-MS) and chemometrics methods were applied to differentiate two species of Asari Radix et Rhizoma by their odors. The samples used were the dried roots and rhizomes of Asarum heterotropoides var. mandshuricum (AH) and Asarum sieboldii (AS). The electronic nose was used to determine the odors of the samples and enabled rapid differentiation of AH and AS when coupled with principal component analysis. Headspace GC-MS was utilized to reveal the differences between the volatile constituents of AH and AS. In all, 54 volatile constituents were identified, and 9 major constituents (eucalyptol, eucarvone, 3,5-dimethoxytoluene, 3,4,5-trimethoxytoluene, methyleugenol, 2,3,5-trimethoxytoluene, croweacin, pentadecane and asaricin) could be used as chemical markers to distinguish these two species. AH contained higher relative contents of eucarvone (1.79-16.76%), 3,5-dimethoxytoluene (6.64-26.52%), 3,4,5-trimethoxytoluene/methyleugenol (6.43-31.67%) and 2,3,5-trimethoxytoluene (1.64-6.66%), whereas AS had higher relative contents of eucalyptol (14.06-24.95%), croweacin (5.64-13.55%), pentadecane (8.44-20.82%) and asaricin (7.03-13.45%). Moreover, AH and AS could be distinguished according to the contents of either all 54 identified volatile constituents or only the 9 major constituents by employing cluster analysis. The proposed method is rapid, simple, eco-friendly and can successfully differentiate these two species of Asari Radix et Rhizoma by their odors. (C) 2013 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000324351200031
项目编号81274073
资助机构General Program of National Natural Science Foundation of China
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52596
专题北京大学药学院
北京大学药学院_化学生物学系
北京大学药学院_天然药物学系
作者单位Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Li, Chen,Xu, Feng,Cao, Chen,et al. Comparative analysis of two species of Asari Radix et Rhizoma by electronic nose, headspace GC-MS and chemometrics[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2013,85:231-238.
APA Li, Chen.,Xu, Feng.,Cao, Chen.,Shang, Ming-Ying.,Zhang, Cui-Ying.,...&Cai, Shao-Qing.(2013).Comparative analysis of two species of Asari Radix et Rhizoma by electronic nose, headspace GC-MS and chemometrics.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,85,231-238.
MLA Li, Chen,et al."Comparative analysis of two species of Asari Radix et Rhizoma by electronic nose, headspace GC-MS and chemometrics".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 85(2013):231-238.
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