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学科主题: 临床医学
题名:
Excessive Intrauterine Fluid Cause Aberrant Implantation and Pregnancy Outcome in Mice
作者: Lu, Shan1; Peng, Hongying2; Zhang, He2; Zhang, Li2; Cao, Qichen2; Li, Rong1,3,4; Zhang, Ying2; Yan, Liying1; Duan, Enkui2; Qiao, Jie1,3,4
刊名: PLOS ONE
发表日期: 2013-10-23
DOI: 10.1371/journal.pone.0078446
卷: 8, 期:10
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: IN-VITRO FERTILIZATION ; UTERINE LUMINAL FLUID ; EMBRYO IMPLANTATION ; ENDOMETRIAL RECEPTIVITY ; ASSISTED REPRODUCTION ; HYDROSALPINX ; IVF ; SALPINGECTOMY ; EXPRESSION ; WOMEN
英文摘要:

The normal intrauterine fluid environment is essential for embryo implantation. In hydrosalpinx patients, the implantation and pregnancy rates are markedly decreased after IVF-embryo transfer, while salpingectomy could significantly improve the pregnancy rates. The leakage of hydrosalpinx fluid into the endometrial cavity was supposed to be the major cause for impaired fertility. However, the underlying mechanisms of hydrosalpinx fluids on implantation and ongoing pregnancy were not fully understood and remain controversial regarding its toxicity. In present study, by infusing different volume of non-toxic fluid (0.9% saline) into uterine lumen before embryo implantation in mice (Day4 08: 30), we found that while the embryos were not "flushed out" from the uteri, the timing of implantation was deferred and normal intrauterine distribution (embryo spacing) was disrupted. The abnormal implantation at early pregnancy further lead to embryo growth retardation, miscarriage and increased pregnancy loss, which is similar to the adverse effects observed in hydrosalpinx patients undergoing IVF-ET. We further examined uterine receptivity related gene expression reported to be involved in human hydrosalpinx (Lif, Hoxa10, Integrin a(v) and beta(3)). The results showed that expression of integrin alpha(v) and beta(3) were increased in the fluid infused mouse uteri, implicating a compensatory effect to cope with the excessive fluid environment. Our data suggested that the adverse effects of excessive non-toxic luminal fluid on pregnancy are primarily due to the mechanical interference for normal timing and location of embryo apposition, which might be the major cause of decreased implantation rate in IVF-ET patients with hydrosalpinx.

语种: 英语
所属项目编号: 81000275 ; 31300957 ; 31300960 ; 2011CB944503 ; 2011CB944401
项目资助者: National Natural Science Foundation of China ; National Basic Research Program of China
WOS记录号: WOS:000326037000113
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52608
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Minist Educ, Key Lab Assisted Reprod, Beijing, Peoples R China
2.Peking Univ, Dept Obstet & Gynecol, Hosp 3, Beijing 100871, Peoples R China
3.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China
4.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing, Peoples R China

Recommended Citation:
Lu, Shan,Peng, Hongying,Zhang, He,et al. Excessive Intrauterine Fluid Cause Aberrant Implantation and Pregnancy Outcome in Mice[J]. PLOS ONE,2013,8(10).
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