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学科主题: 临床医学
题名:
Endogeous sulfur dioxide protects against oleic acid-induced acute lung injury in association with inhibition of oxidative stress in rats
作者: Chen, Siyao1; Zheng, Saijun2; Liu, Zhiwei2; Tang, Chaoshu3,4; Zhao, Bin2; Du, Junbao1,4; Jin, Hongfang1
刊名: LABORATORY INVESTIGATION
发表日期: 2015-02-01
DOI: 10.1038/labinvest.2014.147
卷: 95, 期:2, 页:142-156
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental ; Pathology
研究领域[WOS]: Research & Experimental Medicine ; Pathology
关键词[WOS]: RESPIRATORY-DISTRESS-SYNDROME ; INDUCED MYOCARDIAL INJURY ; PHOSPHOINOSITIDE HYDROLYSIS ; CARDIOVASCULAR-SYSTEM ; CASPASE-3 ACTIVATION ; GUINEA-PIGS ; HYPEROXIA ; MICE ; PATHOGENESIS ; GLUTATHIONE
英文摘要:

The role of endogenous sulfur dioxide (SO2), an efficient gasotransmitter maintaining homeostasis, in the development of acute lung injury (ALI) remains unidentified. We aimed to investigate the role of endogenous SO2 in the pathogenesis of ALI. An oleic acid (OA)-induced ALI rat model was established. Endogenous SO2 levels, lung injury, oxidative stress markers and apoptosis were examined. OA-induced ALI rats showed a markedly downregulated endogenous SO2/aspartate aminotransferase 1 (AAT1)/AAT2 pathway and severe lung injury. Chemical colorimetry assays demonstrated upregulated reactive oxygen species generation and downregulated antioxidant capacity in OA-induced ALI rats. However, SO2 increased endogenous SO2 levels, protected against oxidative stress and alleviated ALI. Moreover, compared with OA-treated cells, in human alveolar epithelial cells SO2 downregulated O-2(-) and O-H(-) generation. In contrast, L-aspartic acid-beta-hydroxamate (HDX, Sigma-Aldrich Corporation), an inhibitor of endogenous SO2 generating enzyme, promoted free radical generation, upregulated poly (ADP-ribose) polymerase expression, activated caspase-3, as well as promoted cell apoptosis. Importantly, apoptosis could be inhibited by the free radical scavengers glutathione (GSH) and N-acetyl-L-cysteine (NAC). The results suggest that SO2/AAT1/AAT2 pathway might protect against the development of OA-induced ALI by inhibiting oxidative stress.

语种: 英语
所属项目编号: 31130030 ; 91439110 ; 31440052 ; 2012CB517806 ; 2011CB503904
项目资助者: National Natural Science Foundation of China ; Major Basic Research Development Program of People&prime ; s Republic of China
WOS记录号: WOS:000348636900003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52616
Appears in Collections:北京大学第一临床医学院_儿科_期刊论文

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作者单位: 1.Beijing Jishuitan Hosp, Dept Emergency, Beijing, Peoples R China
2.Peking Univ, Hosp 1, Dept Pediat, Beijing 100871, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100871, Peoples R China
4.Minist Educ, Dept Gasotransmitters & Cardiovasc Dis, Key Lab Mol Cardiol, Beijing, Peoples R China

Recommended Citation:
Chen, Siyao,Zheng, Saijun,Liu, Zhiwei,et al. Endogeous sulfur dioxide protects against oleic acid-induced acute lung injury in association with inhibition of oxidative stress in rats[J]. LABORATORY INVESTIGATION,2015,95(2):142-156.
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