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|The ameliorating effects of long-term electroacupuncture on cardiovascular remodeling in spontaneously hypertensive rats|
|Huo, Ze-Jun1,2,3; Li, Quan2; Tian, Gui-Hua2,3; Zhou, Chang-Man4; Wei, Xiao-Hong2; Pan, Chun-Shui2; Yang, Lei4; Bai, Yan5,6; Zhang, You-Yi5,6; He, Ke2; Wang, Chuan-She2,7; Li, Zhi-Gang3; Han, Jing-Yan2,7|
|关键词||Cardiac Hypertrophy Electroacupuncture Angiotensin Ii Type 1 Receptor Endothelin-1 Endothelin-1 Type a Receptor No|
|刊名||BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE|
|WOS标题词||Science & Technology|
|类目[WOS]||Integrative & Complementary Medicine|
|研究领域[WOS]||Integrative & Complementary Medicine|
|关键词[WOS]||VENTRICULAR HYPERTROPHY ; PRESSURE-OVERLOAD ; ANGIOTENSIN-II ; EXPRESSION ; FIBROSIS ; HEART ; ENDOTHELIN-1 ; ACUPUNCTURE ; DEFICIENCY ; ACTIVATION|
Background: The purpose of this study was to investigate the inhibitory effects of long-term electroacupuncture at BaiHui (DU20) and ZuSanLi (ST36) on cardiovascular remodeling in spontaneously hypertensive rats (SHR) and underlying mechanisms.
Methods: 6-weeks-old SHR or Wistar male rats were randomly, divided into 6 groups: the control group (SHR/Wistar), the non-acupoint electroacupuncture stimulation group (SHR-NAP/Wistar-NAP) and the electroacupuncture stimulation at DU20 and ST36 group (SHR-AP/Wistar-AP), 24 rats in each group. Rats were treated with or without electroacupuncture at DU20 and ST36, once every other day for a period of 8 weeks. The mean arterial pressure (MAP) was measured once every 2 weeks. By the end of the 8th week, the left ventricular structure and function were assessed by echocardiography. The content of angiotensin II (Ang II), endothelin-1 (ET-1) and nitric oxide (NO) in the plasma was determined using enzyme-linked immunosorbent assay. Histological studies on the heart and the ascending aorta were performed. The expression of angiotensin II type 1 receptor (AT1R), endothelin-1 type A receptor (ETAR), eNOS and iNOS in rat myocardium and ascending aorta was investigated by Western blotting.
Results: The MAP in SHR increased linearly over the observation period and significantly reduced following electroacupuncture as compared with sham control SHR rats, while no difference in MAP was observed in Wistar rats between electroacupuncture and sham control. The aortic wall thickness, cardiac hypertrophy and increased collagen level in SHR were attenuated by long term electroacupuncture. The content of Ang II, ET-1 in the plasma decreased, but the content of NO increased after electroacupuncture stimulation in SHR. Long term electroacupuncture significantly inhibited the expression of AT1R, ETAR and iNOS, whereas increased eNOS expression, in myocardium and ascending aorta of SHR.
Conclusions: The long term electroacupuncture stimulation at DU20 and ST36 relieves the increased MAP and cardiovascular abnormality in both structure and function in SHR, this beneficial action is most likely mediated via modulation of AT1R-AT1R-ET-1-ETAR and NOS/NO pathway.
|资助机构||Production of New Medicine Program of Ministry of Science and Technology of the People&prime ; s Republic of China|
|作者单位||1.Peking Univ, Hosp 3, Dept Tradit Chinese Med, Beijing 100871, Peoples R China|
2.Peking Univ, Hlth Sci Ctr, Tasly Microcirculat Res Ctr, Beijing 100871, Peoples R China
3.Beijing Univ Chinese Med, Sch Acupuncture & Moxibust, Beijing 100029, Peoples R China
4.Peking Univ, Sch Basic Med Sci, Dept Anat, Beijing 100871, Peoples R China
5.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100871, Peoples R China
6.Chinese Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing, Peoples R China
7.Peking Univ, Sch Basic Med Sci, Dept Integrat Chinese & Western Med, Beijing 100191, Peoples R China
|Huo, Ze-Jun,Li, Quan,Tian, Gui-Hua,et al. The ameliorating effects of long-term electroacupuncture on cardiovascular remodeling in spontaneously hypertensive rats[J]. BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE,2014,14.|
|APA||Huo, Ze-Jun.,Li, Quan.,Tian, Gui-Hua.,Zhou, Chang-Man.,Wei, Xiao-Hong.,...&Han, Jing-Yan.(2014).The ameliorating effects of long-term electroacupuncture on cardiovascular remodeling in spontaneously hypertensive rats.BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE,14.|
|MLA||Huo, Ze-Jun,et al."The ameliorating effects of long-term electroacupuncture on cardiovascular remodeling in spontaneously hypertensive rats".BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE 14(2014).|
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