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学科主题: 基础医学
题名:
Acetazolamide inhibits osmotic water permeability by interaction with aquaporin-1
作者: Gao, JW1; Wang, XH1; Chang, YJ1; Zhang, JZ1; Song, QL1; Yu, HM1; Li, XJ1
关键词: acetazolamide ; aquaporin-1 ; osmotic water permeability ; transfected cell ; interaction ; surface plasmon resonance
刊名: ANALYTICAL BIOCHEMISTRY
发表日期: 2006-03-15
DOI: 10.1016/j.ab.2006.01.003
卷: 350, 期:2, 页:165-170
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: BIOSENSOR ANALYSIS ; XENOPUS OOCYTES ; CHANNELS ; CELLS ; PROTEIN ; ERYTHROCYTES ; NEPHRON ; ANTIGEN ; SURFACE
英文摘要:

Water channel proteins, known as aquaporins, are transmembrane proteins that mediate osmotic water permeability. In a previous study, we found that acetazolamide could inhibit osmotic water transportation across Xenopus oocytes by blocking the function of aquaporin-1 (AQP1). The purpose of the current study was to confirm the effect of acetazolamide on water osmotic permeability using the human embryonic kidney 293 (HEK293) cells transfected with pEGFP/AQP1 and to investigate the interaction between acetazolamide and AQP1. The fluorescence intensity of HEK293 cells transfected with pEGFP/AQP1. which corresponds to the cell volume when the cells swell in a hyposmotic solution, was recorded under confocal laser fluorescence microscopy. The osmotic water permeability was assessed by the change in the ratio of cell fluorescence to certain cell area. Acetazolamide, at concentrations of I and 10 mu M, inhibited the osmotic water permeability in HEK293 cells transfected with pEGFP/AQP1. The direct binding between acetazolamide and AQP1 was detected by surface plasmon resonance. AQP1 was prepared from rat red blood cells and immobilized on a CM5 chip. The binding assay showed that acetazolamide Could directly interact with AQP1. This study demonstrated that acetazolamide inhibited osmotic water permeability through interaction with AQP1. (c) 2006 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000236155000001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52632
Appears in Collections:基础医学院_药理学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Pharmacol, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100083, Peoples R China
4.Natl Res Inst Family Planning, Beijing 100081, Peoples R China

Recommended Citation:
Gao, JW,Wang, XH,Chang, YJ,et al. Acetazolamide inhibits osmotic water permeability by interaction with aquaporin-1[J]. ANALYTICAL BIOCHEMISTRY,2006,350(2):165-170.
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