IR@PKUHSC  > 北京大学基础医学院  > 药理学系
学科主题基础医学
Acetazolamide inhibits osmotic water permeability by interaction with aquaporin-1
Gao, JW1; Wang, XH1; Chang, YJ1; Zhang, JZ1; Song, QL1; Yu, HM1; Li, XJ1
关键词Acetazolamide Aquaporin-1 Osmotic Water Permeability Transfected Cell Interaction Surface Plasmon Resonance
刊名ANALYTICAL BIOCHEMISTRY
2006-03-15
DOI10.1016/j.ab.2006.01.003
350期:2页:165-170
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]BIOSENSOR ANALYSIS ; XENOPUS OOCYTES ; CHANNELS ; CELLS ; PROTEIN ; ERYTHROCYTES ; NEPHRON ; ANTIGEN ; SURFACE
英文摘要

Water channel proteins, known as aquaporins, are transmembrane proteins that mediate osmotic water permeability. In a previous study, we found that acetazolamide could inhibit osmotic water transportation across Xenopus oocytes by blocking the function of aquaporin-1 (AQP1). The purpose of the current study was to confirm the effect of acetazolamide on water osmotic permeability using the human embryonic kidney 293 (HEK293) cells transfected with pEGFP/AQP1 and to investigate the interaction between acetazolamide and AQP1. The fluorescence intensity of HEK293 cells transfected with pEGFP/AQP1. which corresponds to the cell volume when the cells swell in a hyposmotic solution, was recorded under confocal laser fluorescence microscopy. The osmotic water permeability was assessed by the change in the ratio of cell fluorescence to certain cell area. Acetazolamide, at concentrations of I and 10 mu M, inhibited the osmotic water permeability in HEK293 cells transfected with pEGFP/AQP1. The direct binding between acetazolamide and AQP1 was detected by surface plasmon resonance. AQP1 was prepared from rat red blood cells and immobilized on a CM5 chip. The binding assay showed that acetazolamide Could directly interact with AQP1. This study demonstrated that acetazolamide inhibited osmotic water permeability through interaction with AQP1. (c) 2006 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000236155000001
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被引频次:48[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52632
专题北京大学基础医学院_药理学系
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Pharmacol, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100083, Peoples R China
4.Natl Res Inst Family Planning, Beijing 100081, Peoples R China
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Gao, JW,Wang, XH,Chang, YJ,et al. Acetazolamide inhibits osmotic water permeability by interaction with aquaporin-1[J]. ANALYTICAL BIOCHEMISTRY,2006,350(2):165-170.
APA Gao, JW.,Wang, XH.,Chang, YJ.,Zhang, JZ.,Song, QL.,...&Li, XJ.(2006).Acetazolamide inhibits osmotic water permeability by interaction with aquaporin-1.ANALYTICAL BIOCHEMISTRY,350(2),165-170.
MLA Gao, JW,et al."Acetazolamide inhibits osmotic water permeability by interaction with aquaporin-1".ANALYTICAL BIOCHEMISTRY 350.2(2006):165-170.
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