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学科主题: 临床医学
题名:
Effects of N-acetylcysteine on Clara cells in rats with cigarette smoke exposure
作者: Liao Ji-ping1; Chi Chun-hua1; Li Hai-chao1; Tang Xiu-ying2
关键词: tobacco smoke pollution ; Clara cells ; N-acetylcysteine ; glutathion
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2010-02-20
DOI: 10.3760/cma.j.issn.0366-6999.2010.04.006
卷: 123, 期:4, 页:412-417
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: OBSTRUCTIVE PULMONARY-DISEASE ; ALVEOLAR EPITHELIAL-CELLS ; SMALL AIRWAYS ; KDA PROTEIN ; INFLAMMATION ; GLUTATHIONE ; EXPRESSION ; STRESS ; FLUID
英文摘要:

Background The number of Clara cells and the Clara cell 16-kDa protein (CC16) levels of the lung decrease inpatients with chronic obstructive pulmonary disease (COPD) N-acetylcysteine (NAC) is a powerful antioxidant and can reduce the frequency of acute exacerbations of COPD But the exact mechanism is unclear The present study was designed to investigate the effects of NAC on Clara cells in rats with cigarette smoke exposure

Methods Eighteen adult male Wistar rats were randomly divided into 3 groups, 12 exposed to cigarette smoke (CS) thrice a day, 10 cigarettes for 30 minutes each time for 1 week, without (CS group) or with (CS+NAC group) oral intake of NAC 80 mg kg(-1) d(-1), and another 6 rats exposed to fresh air (control group) Clara cells were observed by an electron microscope. The mRNA expression of CC16 and CC16 protein in lungs were determined by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry respectively The glutathion (GSH) level in plasma and lung tissue were tested by fluorimetry assay

Results Compared with the controls, the pathologic score of small airways significantly increased in the CS exposed rats (20 3 +/- 14 7 vs 53.7 +/- 11.5, P <0 05). The Clara cell particles in cytoplasm decreased in the CS group (P <0.05) The percentage of CC16-positive cells in bronchioles in the CS group (27 8 +/- 4.3 and 29 5 +/- 2 4 in terminal bronchioles and respiratory bronchioles, respectively) significantly decreased as compared with the control group (37 1 +/- 3.8 and 43 8 +/- 5.8 in terminal bronchioles and respiratory bronchioles, respectively) (P <0 05) No significant difference was observed in GSH level ((18 +/- 26) nmol/L in the control group vs (170 +/- 18) nmol/L in the CS group) between the two groups After treatment with NAC, the pathologic score of small airways (24 1 +/- 17.5) decreased (P <0 05). Clara cell particles in cytoplasm of Clara cells increased and GSH level in plasma ((213 +/- 40) nmol/L vs (170 +/- 18) nmol/L in the CS group) increased too (P <0.05), while the increase in the proportions of CC16 positive cells in bronchioles (30 1 +/- 6.4 and 34 3 +/- 6.3 in terminal bronchioles and respiratory bronchioles, respectively) did not reach the statistical significance (P >0 05) No significant difference was found in the expression of CC16 mRNA among the three groups Correlation analysis indicated that the percentage of CC16-positive cells in bronchioles negatively correlated with the pathologic score of small airways (r = -0 592, P <0.05), but not with GSH level

Conclusions One-week CS exposure decreased the number of Clara cells and the expression of CC16 in bronchiciles in rats NAC might provide protection of the Clara cells from oxidative damage and possibly through the elevation of the synthesis and secretion of CC16. These data indicate that NAC decreases airway inflammation induced by CS via induction of CC16 Chin Med J 2010,-123(4).412-417

语种: 英语
WOS记录号: WOS:000275048100006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52637
Appears in Collections:北京大学第一临床医学院_呼吸和危重症医学科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Dept Resp Med, Beijing 100034, Peoples R China
2.Peking Univ, Hosp 1, Dept Pathol, Beijing 100034, Peoples R China

Recommended Citation:
Liao Ji-ping,Chi Chun-hua,Li Hai-chao,et al. Effects of N-acetylcysteine on Clara cells in rats with cigarette smoke exposure[J]. CHINESE MEDICAL JOURNAL,2010,123(4):412-417.
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