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学科主题临床医学
Monoclonal side population progenitors isolated from human fetal pancreas
Zhang, L1; Hu, J1; Hong, TP1; Liu, YN1; Wu, YH1; Li, LS1
关键词Abc Transporter Fetal Insulin-secreting Cells Pancreas Side Population Stem Cells
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2005-07-29
DOI10.1016/j.bbrc.2005.05.111
333期:2页:603-608
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]EMBRYONIC STEM-CELLS ; NESTIN-POSITIVE PROGENITOR ; INSULIN-PRODUCING CELLS ; BONE-MARROW ; IN-VITRO ; ENDOCRINE-CELLS ; DIABETIC MICE ; BETA-CELLS ; DIFFERENTIATION ; ISLETS
英文摘要

The side population (SP) phenotype might represent a common molecular feature for a wide variety of stem cells. The aim of this study was to investigate whether monoclonal SP progenitor cells were established from human fetal pancreas. Islet-like cell clusters (ICCs) were isolated from human fetal pancreas. Monolayer epithelium-like cells were obtained from the ICCs and passaged thereafter. Single SP or non-SP cells were sorted from these cells at the sixth passage. The rate of clone formation was about 2.7% for the SP cells, whereas there was no clone formation for the non-SP cells. The SP cell clones were further expanded for more than 15 passages and induced for differentiation into cells with characteristics of pancreatic P-cells. We show for the first timed that the monoclonal SP progenitors are established from human fetal pancreas. Therefore, this study may offer a novel method to purify pancreatic progenitor cells from human tissues. (c) 2005 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000230418700045
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52643
专题北京大学口腔医学院_儿童口腔科
北京大学基础医学院
北京大学第三临床医学院_内分泌科
作者单位1.Peking Univ, Hosp 3, Dept Endocrinol, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Stem Cell Res Ctr, Beijing 100083, Peoples R China
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GB/T 7714
Zhang, L,Hu, J,Hong, TP,et al. Monoclonal side population progenitors isolated from human fetal pancreas[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2005,333(2):603-608.
APA Zhang, L,Hu, J,Hong, TP,Liu, YN,Wu, YH,&Li, LS.(2005).Monoclonal side population progenitors isolated from human fetal pancreas.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,333(2),603-608.
MLA Zhang, L,et al."Monoclonal side population progenitors isolated from human fetal pancreas".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 333.2(2005):603-608.
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