|Naloxone Ameliorates Retinal Lesions in Ccl2/Cx3cr1 Double-Deficient Mice via Modulation of Microglia|
|Shen, Defen1; Cao, Xiaoguang1,3; Zhao, Lian2; Tuo, Jingsheng1; Wong, Wai T.2; Chan, Chi-Chao1|
|刊名||INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE|
|WOS标题词||Science & Technology|
|关键词[WOS]||INDUCED PHOTORECEPTOR DEGENERATION ; MACULAR DEGENERATION ; NITRIC-OXIDE ; NEURODEGENERATIVE DISEASES ; THERAPEUTIC INTERVENTION ; INTRAOCULAR-PRESSURE ; SUBRETINAL MICROGLIA ; ACTIVATED MICROGLIA ; PARKINSONS-DISEASE ; OPIOID RECEPTOR|
PURPOSE. The role of naloxone, an opioid receptor antagonist, on microglial inhibition and neuroprotective effects has been reported in lipopolysaccharide (LPS)-induced neurodegeneration and light-induced photoreceptor degeneration. The authors evaluated the effects of naloxone on Ccl2(-/-)/Cx3cr1(-/-) (DKO) mice, a murine model of age-related macular degeneration (AMD).
METHODS. Two-month-old DKO and wild-type controls were given daily intraperitoneal injections of naloxone or PBS for 2 months. Animals were examined monthly by funduscopy. Ocular tissue was analyzed histologically and in retinal flat mount preparations. Ocular A2E was measured using HPLC. Quantitative RT-PCR analyzed TNF-alpha, IL-1 beta, IL-10 and TLR4 transcripts in the DKO eyes and LPS activated culture microglial cells. Serum nitrite was measured using Griess colorimetric reaction.
RESULTS. Naloxone ameliorated the clinical progression and severity of retinal lesions in the DKO mice compared with those of untreated controls. Histopathology also showed less focal retinal degeneration in the treated DKO mice than in controls. The aggregation of microglia in the outer retina in DKO mice was significantly reduced in naloxone-treated animals compared with control untreated DKO. Ocular TNF-alpha, IL-1 beta, and TLR4 transcripts and A2E were significantly lower in naloxone-treated DKO animals and cultured microglial cells than in controls, as were serum nitrite levels.
CONCLUSIONS. Naloxone significantly reduces the progress of retinal lesions in DKO mice. Naloxone modulates microglia accumulation and activation at the site of retinal degeneration, which may be mediated by inhibition of the proinflammatory molecules of NO, TNF-alpha, and IL-beta. The potential therapeutic effects of naloxone on retinal degeneration, including AMD, warrants further investigation. (Invest Ophthalmol Vis Sci. 2011;52:2897-2904) DOI:10.1167/iovs.10-6114
|资助机构||National Eye Institute|
|作者单位||1.NEI, Immunopathol Sect, Immunol Lab, NIH, Bethesda, MD 20892 USA|
2.NEI, Unit Neuronal & Glial Interact Retinal Dis, NIH, Bethesda, MD 20892 USA
3.Peking Univ, Dept Ophthalmol, Peoples Hosp, Beijing 100871, Peoples R China
|Shen, Defen,Cao, Xiaoguang,Zhao, Lian,et al. Naloxone Ameliorates Retinal Lesions in Ccl2/Cx3cr1 Double-Deficient Mice via Modulation of Microglia[J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,2011,52(6):2897-2904.|
|APA||Shen, Defen,Cao, Xiaoguang,Zhao, Lian,Tuo, Jingsheng,Wong, Wai T.,&Chan, Chi-Chao.(2011).Naloxone Ameliorates Retinal Lesions in Ccl2/Cx3cr1 Double-Deficient Mice via Modulation of Microglia.INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,52(6),2897-2904.|
|MLA||Shen, Defen,et al."Naloxone Ameliorates Retinal Lesions in Ccl2/Cx3cr1 Double-Deficient Mice via Modulation of Microglia".INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 52.6(2011):2897-2904.|