IR@PKUHSC  > 北京大学基础医学院  > 药理学系
学科主题基础医学
Sinomenine influences capacity for invasion and migration in activated human monocytic THP-1 cells by inhibiting the expression of MMP-2, MMP-9, and CD147
Ou, Yang-qiong; Chen, Li-hua; Li, Xue-jun; Lin, Zhi-bin; Li, Wei-dong
关键词Sinomenine Rheumatoid Arthritis Invasion Migration Cd147 Mmp-2 Mmp-9
刊名ACTA PHARMACOLOGICA SINICA
2009-04-01
DOI10.1038/aps.2009.21
30期:4页:435-441
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]COLLAGENASE-STIMULATORY FACTOR ; CANCER CELL ; HUMAN LUNG ; ARTHRITIS ; GROWTH ; RATS
英文摘要

Aim: The aim of this study was to investigate the mechanism of the effects of Sinomenine (SIN) on the invasion and migration ability of activated human monocytic THP-1 cells (A-THP-1). Sinomenine is a pure alkaloid extracted from the Chinese medical plant Sinomenium acutum.

Methods: Human monocytic THP-1 cells were induced to differentiate into macrophages with phorbol 12-myristate 13-acetate (PMA). Cells were treated with different concentrations of SIN. The invasion and migration ability of cells was tested by in vitro transwell assays. The levels of CD147 and MMPs were evaluated by flow cytometric analysis and zymographic analysis, respectively. The mRNA expression of CD147, MMP-2, and MMP-9 was measured by RT-PCR.

Results: The invasion and migration ability of A-THP-1 cells was significantly inhibited by SIN in a concentration-dependent fashion; at the same time, the levels of CD147, MMP-2, and MMP-9 were markedly down-regulated. This inhibitory effect was most notable at concentrations of 0.25 mmol/L and 1.00 mmol/L (P<0.01).

Conclusion: A possible mechanism of the inhibitory effect of SIN on cell invasion and migration ability is repression of the expression of MMP-2 and MMP-9, which strongly correlates with the inhibition of CD147 activity.

语种英语
WOS记录号WOS:000265151100007
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52649
专题北京大学基础医学院_药理学系
北京大学基础医学院
作者单位Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Pharmacol, Beijing 100191, Peoples R China
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GB/T 7714
Ou, Yang-qiong,Chen, Li-hua,Li, Xue-jun,et al. Sinomenine influences capacity for invasion and migration in activated human monocytic THP-1 cells by inhibiting the expression of MMP-2, MMP-9, and CD147[J]. ACTA PHARMACOLOGICA SINICA,2009,30(4):435-441.
APA Ou, Yang-qiong,Chen, Li-hua,Li, Xue-jun,Lin, Zhi-bin,&Li, Wei-dong.(2009).Sinomenine influences capacity for invasion and migration in activated human monocytic THP-1 cells by inhibiting the expression of MMP-2, MMP-9, and CD147.ACTA PHARMACOLOGICA SINICA,30(4),435-441.
MLA Ou, Yang-qiong,et al."Sinomenine influences capacity for invasion and migration in activated human monocytic THP-1 cells by inhibiting the expression of MMP-2, MMP-9, and CD147".ACTA PHARMACOLOGICA SINICA 30.4(2009):435-441.
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