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Activation of the Nrf2/HO-1 Antioxidant Pathway Contributes to the Protective Effects of Lycium Barbarum Polysaccharides in the Rodent Retina after Ischemia-Reperfusion-Induced Damage
He, Meihua1,2,3; Pan, Hong1,2,3; Chang, Raymond Chuen-Chung4,5,6; So, Kwok-Fai4,5,6,7; Brecha, Nicholas C.8,9,10; Pu, Mingliang1,2,3
刊名PLOS ONE
2014-01-06
DOI10.1371/journal.pone.0084800
9期:1
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者National Basic Research Program of China (973 Program) ; National Science Foundation of China ; Ministry of Education of China ; Azalea Endowment Fund ; Fundamental Research Fund for the Central Universities ; NIH ; VA Merit Review ; National Basic Research Program of China (973 Program) ; National Science Foundation of China ; Ministry of Education of China ; Azalea Endowment Fund ; Fundamental Research Fund for the Central Universities ; NIH ; VA Merit Review
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]INDUCED OXIDATIVE STRESS ; HEME OXYGENASE-1 ; CAPILLARY DEGENERATION ; GLUCOSE DEPRIVATION ; GENE-EXPRESSION ; CARBON-MONOXIDE ; FREE-RADICALS ; IN-VIVO ; INJURY ; CELLS
英文摘要

Lycium barbarum polysaccharides (LBP), extracts from the wolfberries, are protective to retina after ischemia-reperfusion (I/R). The antioxidant response element (ARE)-mediated antioxidant pathway plays an important role in maintaining the redox status of the retina. Heme oxygenase-1 (HO-1), combined with potent AREs in its promoter, is a highly effective therapeutic target for the protection against neurodegenerative diseases, including I/R-induced retinal damage. The aim of our present study was to investigate whether the protective effect of LBP after I/R damage was mediated via activation of the Nrf2/HO-1-antioxidant pathway in the retina. Retinal I/R was induced by an increase in intraocular pressure to 130 mm Hg for 60 minutes. Prior to the induction of ischemia, rats were orally treated with either vehicle (PBS) or LBP (1 mg/kg) once a day for 1 week. For specific experiments, zinc protoporphyrin (ZnPP, 20 mg/kg), an HO-1 inhibitor, was intraperitoneally administered at 24 h prior to ischemia. The protective effects of LBP were evaluated by quantifying ganglion cell and amacrine cell survival, and by measuring cell apoptosis in the retinal layers. In addition, HO-1 expression was examined using Western blotting and immunofluorescence analyses. Cytosolic and nuclear Nrf2 was measured using immunofluorescent staining. LBP treatment significantly increased Nrf2 nuclear accumulation and HO-1 expression in the retina after I/R injury. Increased apoptosis and a decrease in the number of viable cells were observed in the ganglion cell layer (GCL) and inner nuclear layer (INL) in the I/R retina, which were reversed by LBP treatment. The HO-1 inhibitor, ZnPP, diminished the LBP treatment-induced protective effects in the retina after I/R. Taken together, these results suggested that LBP partially exerted its beneficial neuroprotective effects via the activation of Nrf2 and an increase in HO-1 protein expression.

语种英语
所属项目编号2009CB320900 ; 2011CB510206 ; 30831160516 ; 81200691 ; 20090001120075 ; 21609101 ; EY04067
资助者National Basic Research Program of China (973 Program) ; National Science Foundation of China ; Ministry of Education of China ; Azalea Endowment Fund ; Fundamental Research Fund for the Central Universities ; NIH ; VA Merit Review ; National Basic Research Program of China (973 Program) ; National Science Foundation of China ; Ministry of Education of China ; Azalea Endowment Fund ; Fundamental Research Fund for the Central Universities ; NIH ; VA Merit Review
WOS记录号WOS:000329462700048
引用统计
被引频次:52[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52650
专题基础医学院
作者单位1.LKS Fac Med, Dept Anat, Pokfulam, Hong Kong, Peoples R China
2.Greater Los Angeles Hlth Syst, Vet Adm, Los Angeles, CA USA
3.Peking Univ, Sch Basic Med Sci, Dept Anat Embryol, Beijing 100871, Peoples R China
4.Peking Univ, Minist Educ, Key Lab Machine Percept, Beijing 100871, Peoples R China
5.Peking Univ, Minist Educ, Key Lab Visual Impairment & Restorat, Beijing 100871, Peoples R China
6.LKS Fac Med, Res Ctr Heart Brain Hormone & Hlth Aging, Pokfulam, Hong Kong, Peoples R China
7.Univ Hong Kong, State Key Lab Brain & Cognit Sci, Pokfulam, Hong Kong, Peoples R China
8.Jinan Univ, GHM Inst CNS Regenerat, Guangzhou, Guangdong, Peoples R China
9.Univ Hong Kong, Dept Ophthalmol, Pokfulam, Hong Kong, Peoples R China
10.Univ Calif Los Angeles, Jules Stein Eye Inst, David Geffen Sch Med Los Angeles, CURE Digest Dis Res Ctr,Dept Neurobiol,Dept Med, Los Angeles, CA 90024 USA
推荐引用方式
GB/T 7714
He, Meihua,Pan, Hong,Chang, Raymond Chuen-Chung,et al. Activation of the Nrf2/HO-1 Antioxidant Pathway Contributes to the Protective Effects of Lycium Barbarum Polysaccharides in the Rodent Retina after Ischemia-Reperfusion-Induced Damage[J]. PLOS ONE,2014,9(1).
APA He, Meihua,Pan, Hong,Chang, Raymond Chuen-Chung,So, Kwok-Fai,Brecha, Nicholas C.,&Pu, Mingliang.(2014).Activation of the Nrf2/HO-1 Antioxidant Pathway Contributes to the Protective Effects of Lycium Barbarum Polysaccharides in the Rodent Retina after Ischemia-Reperfusion-Induced Damage.PLOS ONE,9(1).
MLA He, Meihua,et al."Activation of the Nrf2/HO-1 Antioxidant Pathway Contributes to the Protective Effects of Lycium Barbarum Polysaccharides in the Rodent Retina after Ischemia-Reperfusion-Induced Damage".PLOS ONE 9.1(2014).
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