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学科主题: 药学
题名:
Synthesis, biocompatibility and cell labeling of L-arginine-functional beta-cyclodextrin-modified quantum dot probes
作者: Zhao, Mei-Xia3; Xia, Qing1; Feng, Xu-Dong1; Zhu, Xu-Hui2; Mao, Zong-Wan1,3; Ji, Liang-Nian3; Wang, Kui1
关键词: QDs ; beta-CD-L-Arg ; Biocompatibility ; Cytotoxicity ; Bio-imaging
刊名: BIOMATERIALS
发表日期: 2010-05-01
DOI: 10.1016/j.biomaterials.2010.01.114
卷: 31, 期:15, 页:4401-4408
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]: Engineering ; Materials Science
关键词[WOS]: CDSE/CDS CORE/SHELL NANOCRYSTALS ; HIGHLY LUMINESCENT ; EPITAXIAL-GROWTH ; WATER ; CYTOTOXICITY ; COMPLEXATION ; PROTEINS ; DESIGN ; PHASE ; CORE
英文摘要:

A series of quantum dots (QDs), CdSe, CdSe/CdS and CdSe/ZnSe, coated with L-arginine-modified beta-cyclodextrin (beta-CD-L-Arg) were prepared in a solution of H2O and hexane by ultrasonic method and characterized using PL, UV-vis, TEM, EDX and FTIR techniques. We observed that beta-CD-L-Arg-coated QDs are water-soluble and stable with high colloidal properties in water. Their photophysical properties are similar to those of trioctylphosphine oxide (TOPO)-coated nanocrystals. The quantum yield (QY) of beta-CD-L-Arg/CdSe/ZnSe QDs in water is 68%, which is much higher than those of beta-CD-L-Arg/CdSe/CdS (26%) and beta-CD-L-Arg/CdSe (13%). The in vitro cytotoxicity of these QDs was evaluated in ECV-304, SH-SY5Y and Hela cells and low cytotoxicity was observed. In particular, the beta-CD-L-Arg/CdSe/ZnSe QDs presented lower cytotoxicity to these cells (CC(50) value is 173 mu g/mL in ECV-304 cells for 48 h). This may be due to the presence of the ZnSe and beta-CD-L-Arg outlayer, which may improve the biocompatibility of QDs. The QDs were further investigated for biological labeling in ECV-304 cells using confocal laser scanning fluorescence microscopy. We found that these QDs were capable of localing to the cytoplasm of cells. These results demonstrate that the beta-CD-L-Arg-coated QDs could be used as a potential photoluminescent nanocrystal probing agent with good biocompatibility. (C) 2010 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 30770494 ; 20725103 ; 20831006 ; 20821001 ; 20901005 ; 9351027501000003 ; 2007CB815306
项目资助者: National Natural Science Foundation of China ; Guangdong Provincial Natural Science Foundation ; National Basic Research Program of China
WOS记录号: WOS:000276932600018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52651
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, State Key Lab Nat & Biomimet Drug, Beijing 100191, Peoples R China
2.S China Univ Technol, Sch Mat Sci Engn, Guangzhou 510641, Guangdong, Peoples R China
3.Sun Yat Sen Univ, Sch Chem & Chem Engn, MOE Key Lab Bioinorgan & Synthet Chem, Guangzhou 510275, Guangdong, Peoples R China

Recommended Citation:
Zhao, Mei-Xia,Xia, Qing,Feng, Xu-Dong,et al. Synthesis, biocompatibility and cell labeling of L-arginine-functional beta-cyclodextrin-modified quantum dot probes[J]. BIOMATERIALS,2010,31(15):4401-4408.
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