北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学临床肿瘤学院  > 肿瘤放疗科  > 期刊论文
学科主题: 临床医学
题名:
Chloroquine-mediated radiosensitization is due to the destabilization of the lysosomal membrane and subsequent induction of cell death by necrosis
作者: Zhao, H1; Cai, Y1; Santi, S1; Lafrenie, R1; Lee, H1
刊名: RADIATION RESEARCH
发表日期: 2005-09-01
卷: 164, 期:3, 页:250-257
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biology ; Biophysics ; Radiology, Nuclear Medicine & Medical Imaging
研究领域[WOS]: Life Sciences & Biomedicine - Other Topics ; Biophysics ; Radiology, Nuclear Medicine & Medical Imaging
关键词[WOS]: RADIATION-INDUCED APOPTOSIS ; CERAMIDE-INDUCED APOPTOSIS ; STRESS-INDUCED APOPTOSIS ; BREAST-CANCER CELLS ; CATHEPSIN-D ; ASCITES HEPATOMA ; DOWN-REGULATION ; GROWTH-STATE ; EXOCYTOSIS ; GENERATION
英文摘要:

The anti-malarial drug chloroquine (CQ) is also thought to be a potential radiation sensitizer. To gain a better understanding of how the lysomotropic CQ can potentiate the effects of ionizing radiation, we investigated the effects of CQ on lysosomal and mitochondrial membrane stability, the subcellular localization of ceramide, plasma membrane permeability, and the mode of cell death in response to irradiation. We found that CQ accumulated in the lysosomes and thus lysosomal volumes increased. As a result, both the lysosomal and plasma membranes were destabilized. After 7 Gy irradiation, most ceramide was associated with the lysosomes in the cells treated with CQ but not in the CQ-untreated control. The elevated levels of ceramide in the lysosomes of the CQ-treated cells appeared to further destabilize the lysosomal and plasma membranes of the cell. Both CQ-treated and -untreated cells had approximately the same rate of cell death by apoptosis after 7 Gy irradiation (P > 0.05, ns). However, in contrast to the CQ-untreated control, the CQ-treated cells underwent massive cell death by necrosis at 24-48 h after irradiation (P < 0.05). Taken together, our data support the idea that the increase in cytotoxic effects by the combination of CQ and radiation is due to radiation-mediated apoptosis and CQ-mediated necrosis. (c) 2005 by Radiation Research Society.

语种: 英语
WOS记录号: WOS:000231551500004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52662
Appears in Collections:北京大学临床肿瘤学院_肿瘤放疗科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.NE Ontario Reg Canc Ctr, Dept Res, Sudbury, ON P3E 5J1, Canada
2.Peking Univ, Sch Oncol, Dept Radiat Oncol, Beijing Canc Hosp, Beijing 10036, Peoples R China

Recommended Citation:
Zhao, H,Cai, Y,Santi, S,et al. Chloroquine-mediated radiosensitization is due to the destabilization of the lysosomal membrane and subsequent induction of cell death by necrosis[J]. RADIATION RESEARCH,2005,164(3):250-257.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhao, H]'s Articles
[Cai, Y]'s Articles
[Santi, S]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhao, H]‘s Articles
[Cai, Y]‘s Articles
[Santi, S]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace