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学科主题: 临床医学
题名:
A label-free method for studying DNA sequence recognition of mitoxantrone based on resonance light-scattering technique
作者: Chen, Zhanguang1; Zhang, Guomin1; Chen, Xi2; Chen, Junhui3; Liu, Jinbin4
关键词: anticancer drugs ; mitoxantrone ; oligonucleotides ; recognition ; resonance light scattering ; selectivity
刊名: JOURNAL OF ANTIBIOTICS
发表日期: 2012-10-01
DOI: 10.1038/ja.2012.58
卷: 65, 期:10, 页:517-522
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biotechnology & Applied Microbiology ; Immunology ; Microbiology ; Pharmacology & Pharmacy
研究领域[WOS]: Biotechnology & Applied Microbiology ; Immunology ; Microbiology ; Pharmacology & Pharmacy
关键词[WOS]: ANTICANCER DRUG ; NANOPARTICLES ; DISTORTIONS ; CISPLATIN ; INDICATOR ; BINDING ; AGENTS ; SERUM ; ACIDS
英文摘要:

A label-free method for studying DNA sequence recognition of mitoxantrone (MIT) by resonance light-scattering (RLS) technique has been developed in this contribution. Through the RLS spectra, the selective no-covalent interactions between MIT and double-stranded DNA, single-stranded DNA (ssDNA), oligonucleotides were systematically studied. The results of the experiments displayed that MIT had an obvious preference to ssDNA with the K (K-RLS), 15.16 mmol mg(-1) and the number of binding sites (N), 8.68 x 10(-4) mmol mg(-1). Besides, it was found that MIT had a preference to sequences that were rich in guanine and cytosine bases with K-RLS, 17.29 l mmol(-1) and N, 1.19 x 10(-2) l mmol(-1). The recognition mechanisms were well discussed and by fluorescence method and atom force microscopy, the RLS technique was confirmed to be a reliable method in this study. Compared with other methods, what the RLS strategy displayed was the direct interaction between anticancer drugs and DNA in vitro without the influence of a foreign substance. Thus, it can be a simple, fast and label-free strategy for DNA sequence recognition studies of DNA-targeted anticancer drugs. The Journal of Antibiotics (2012) 65, 517-522; doi:10.1038/ja.2012.58; published online 18 July 2012

语种: 英语
所属项目编号: 39670298 ; YR09009
项目资助者: National Natural Science Foundation of China ; Science Foundation of Shantou University
WOS记录号: WOS:000310564800007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52670
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Shantou Univ, Dept Chem, Shantou 515063, Peoples R China
2.Univ Texas Dallas, Dept Chem, Richardson, TX 75083 USA
3.Guangdong Pharmaceut Univ, Guangzhou, Guangdong, Peoples R China
4.Peking Univ, Shenzhen Hosp, Dept Radiol, Shenzhen, Peoples R China

Recommended Citation:
Chen, Zhanguang,Zhang, Guomin,Chen, Xi,et al. A label-free method for studying DNA sequence recognition of mitoxantrone based on resonance light-scattering technique[J]. JOURNAL OF ANTIBIOTICS,2012,65(10):517-522.
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