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学科主题: 临床医学
题名:
A New Perfusion Model for Studying Erectile Function
作者: Zhao, Chen1,2,3,4; Chae, Han Jung5; Kim, Suhn Hee6; Cui, Wan Shou7; Lee, Sung Won8; Jeon, Ju-Hong9; Park, Jong Kwan1,2,3,4
关键词: Basic Science Research in Erectile Dysfunction ; In Vitro Penile Perfusion Model ; Intracavernosum Pressure (ICP) ; Tension ; Cyclic Nucleotides ; One-Step Procedure ; Perfusate
刊名: JOURNAL OF SEXUAL MEDICINE
发表日期: 2010-04-01
DOI: 10.1111/j.1743-6109.2009.01652.x
卷: 7, 期:4, 页:1419-1428
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Urology & Nephrology
研究领域[WOS]: Urology & Nephrology
关键词[WOS]: RABBIT CORPUS CAVERNOSUM ; ENDOTHELIUM-DEPENDENT RELAXATION ; NITRIC-OXIDE ; SMOOTH-MUSCLE ; SIGNALING PATHWAY ; IN-VIVO ; MOUSE ; CGMP ; DYSFUNCTION ; INHIBITORS
英文摘要:

Introduction.

The present study introduces a newly devised in vitro penile perfusion model, which allows the measurement of changes in intracavernosum pressure (ICP), tension, and cyclic nucleotides in penile tissue in a one-step procedure.

Aims.

We compared the penile perfusion model with the classical penile strip chamber model to develop the new model.

Methods.

We investigated the role of nitric oxide-cyclic guanosine monophosphate pathway in both systems activated by acetylcholine or electrical field stimulation (EFS). We measured cGMP (cyclic guanosine monophosphate) in the penile corpus cavernosum smooth muscle in both systems and perfusates in the penile perfusion model. We determined ICP, tension, and cGMP simultaneously in penile perfusion model during the experiments.

Main Outcome Measures.

Changes of tension and cGMP in both models, and of cGMP in perfusates and of ICP in penile perfusion model.

Results.

In both systems, acetylcholine relaxed the phenylephrine-precontracted penile corpus cavernosum smooth muscle in a concentration-dependent manner. EFS decreased the tension in both models and ICP in the penile perfusion model in a frequency-dependent manner. Pretreatment with atropine, N omega nitro-L-arginine-methyl ester (L-NAME), and 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) blocked the acetylcholine-induced decrease in the tension in both models and ICP in penile perfusion model. Acetylcholine increased cGMP levels in the perfusate of penile perfusion model and the changes were attenuated by tetrodotoxin (TTX), atropine, L-NAME and ODQ. EFS increased cGMP levels of the penile corpus cavernosum smooth muscle of both models and the changes were attenuated by TTX, atropine, L-NAME, and ODQ.

Conclusion.

The results indicated that the responses of penile strip and perfused penile tissue to acetylcholine and EFS were very similar in both models in terms of tension and cGMP levels. Furthermore, the new model has more advantages over the classical penile strip chamber model in measuring intracavernosum metabolic changes and ICP without interruption of the procedure. Zhao C, Chae HJ, Kim SH, Cui WS, Lee SW, Jeon J-H, and Park JK. A new perfusion model for studying erectile function. J Sex Med 2010;7:1419-1428.

语种: 英语
所属项目编号: A090583
项目资助者: Ministry for Health, Welfare &amp ; Family Affairs, Republic of Korea
WOS记录号: WOS:000276468300010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52671
Appears in Collections:北京大学第一临床医学院_男科中心_期刊论文

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作者单位: 1.Chonbuk Natl Univ, Sch Med, Dept Urol, Jeonju 561712, South Korea
2.Chonbuk Natl Univ, Inst Med Sci, Jeonju 561712, South Korea
3.Chonbuk Natl Univ Hosp, Res Inst Clin Med, Jeonju 561712, South Korea
4.Chonbuk Natl Univ Hosp, CTC Med Device, Jeonju 561712, South Korea
5.Chonbuk Natl Univ, Sch Med, Dept Pharmacol, Jeonju, South Korea
6.Chonbuk Natl Univ, Sch Med, Dept Physiol, Jeonju 561712, South Korea
7.Peking Univ, Androl Ctr, Hosp 1, Beijing, Peoples R China
8.Sungkyunkwan Univ, Sch Med, Dept Urol, Seoul, South Korea
9.Seoul Natl Univ, Sch Med, Dept Physiol, Seoul, South Korea

Recommended Citation:
Zhao, Chen,Chae, Han Jung,Kim, Suhn Hee,et al. A New Perfusion Model for Studying Erectile Function[J]. JOURNAL OF SEXUAL MEDICINE,2010,7(4):1419-1428.
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