IR@PKUHSC  > 北京大学第一临床医学院  > 男科中心
学科主题临床医学
A New Perfusion Model for Studying Erectile Function
Zhao, Chen1,2,3,4; Chae, Han Jung5; Kim, Suhn Hee6; Cui, Wan Shou7; Lee, Sung Won8; Jeon, Ju-Hong9; Park, Jong Kwan1,2,3,4
关键词Basic Science Research In Erectile Dysfunction In Vitro Penile Perfusion Model Intracavernosum Pressure (Icp) Tension Cyclic Nucleotides One-step Procedure Perfusate
刊名JOURNAL OF SEXUAL MEDICINE
2010-04-01
DOI10.1111/j.1743-6109.2009.01652.x
7期:4页:1419-1428
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Urology & Nephrology
资助者Ministry for Health, Welfare &amp ; Family Affairs, Republic of Korea ; Ministry for Health, Welfare &amp ; Family Affairs, Republic of Korea
研究领域[WOS]Urology & Nephrology
关键词[WOS]RABBIT CORPUS CAVERNOSUM ; ENDOTHELIUM-DEPENDENT RELAXATION ; NITRIC-OXIDE ; SMOOTH-MUSCLE ; SIGNALING PATHWAY ; IN-VIVO ; MOUSE ; CGMP ; DYSFUNCTION ; INHIBITORS
英文摘要

Introduction.

The present study introduces a newly devised in vitro penile perfusion model, which allows the measurement of changes in intracavernosum pressure (ICP), tension, and cyclic nucleotides in penile tissue in a one-step procedure.

Aims.

We compared the penile perfusion model with the classical penile strip chamber model to develop the new model.

Methods.

We investigated the role of nitric oxide-cyclic guanosine monophosphate pathway in both systems activated by acetylcholine or electrical field stimulation (EFS). We measured cGMP (cyclic guanosine monophosphate) in the penile corpus cavernosum smooth muscle in both systems and perfusates in the penile perfusion model. We determined ICP, tension, and cGMP simultaneously in penile perfusion model during the experiments.

Main Outcome Measures.

Changes of tension and cGMP in both models, and of cGMP in perfusates and of ICP in penile perfusion model.

Results.

In both systems, acetylcholine relaxed the phenylephrine-precontracted penile corpus cavernosum smooth muscle in a concentration-dependent manner. EFS decreased the tension in both models and ICP in the penile perfusion model in a frequency-dependent manner. Pretreatment with atropine, N omega nitro-L-arginine-methyl ester (L-NAME), and 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) blocked the acetylcholine-induced decrease in the tension in both models and ICP in penile perfusion model. Acetylcholine increased cGMP levels in the perfusate of penile perfusion model and the changes were attenuated by tetrodotoxin (TTX), atropine, L-NAME and ODQ. EFS increased cGMP levels of the penile corpus cavernosum smooth muscle of both models and the changes were attenuated by TTX, atropine, L-NAME, and ODQ.

Conclusion.

The results indicated that the responses of penile strip and perfused penile tissue to acetylcholine and EFS were very similar in both models in terms of tension and cGMP levels. Furthermore, the new model has more advantages over the classical penile strip chamber model in measuring intracavernosum metabolic changes and ICP without interruption of the procedure. Zhao C, Chae HJ, Kim SH, Cui WS, Lee SW, Jeon J-H, and Park JK. A new perfusion model for studying erectile function. J Sex Med 2010;7:1419-1428.

语种英语
所属项目编号A090583
资助者Ministry for Health, Welfare &amp ; Family Affairs, Republic of Korea ; Ministry for Health, Welfare &amp ; Family Affairs, Republic of Korea
WOS记录号WOS:000276468300010
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52671
Collection北京大学第一临床医学院_男科中心
作者单位1.Chonbuk Natl Univ, Sch Med, Dept Urol, Jeonju 561712, South Korea
2.Chonbuk Natl Univ, Inst Med Sci, Jeonju 561712, South Korea
3.Chonbuk Natl Univ Hosp, Res Inst Clin Med, Jeonju 561712, South Korea
4.Chonbuk Natl Univ Hosp, CTC Med Device, Jeonju 561712, South Korea
5.Chonbuk Natl Univ, Sch Med, Dept Pharmacol, Jeonju, South Korea
6.Chonbuk Natl Univ, Sch Med, Dept Physiol, Jeonju 561712, South Korea
7.Peking Univ, Androl Ctr, Hosp 1, Beijing, Peoples R China
8.Sungkyunkwan Univ, Sch Med, Dept Urol, Seoul, South Korea
9.Seoul Natl Univ, Sch Med, Dept Physiol, Seoul, South Korea
Recommended Citation
GB/T 7714
Zhao, Chen,Chae, Han Jung,Kim, Suhn Hee,et al. A New Perfusion Model for Studying Erectile Function[J]. JOURNAL OF SEXUAL MEDICINE,2010,7(4):1419-1428.
APA Zhao, Chen.,Chae, Han Jung.,Kim, Suhn Hee.,Cui, Wan Shou.,Lee, Sung Won.,...&Park, Jong Kwan.(2010).A New Perfusion Model for Studying Erectile Function.JOURNAL OF SEXUAL MEDICINE,7(4),1419-1428.
MLA Zhao, Chen,et al."A New Perfusion Model for Studying Erectile Function".JOURNAL OF SEXUAL MEDICINE 7.4(2010):1419-1428.
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