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学科主题: 临床医学
题名:
Application of hTERC in thinprep samples with mild cytologic abnormality and HR-HPV positive
作者: Li, Ying1; Zeng, Wen-Jie1; Ye, Feng1; Wang, Xin-Yu1; Lue, Wei-Guo1; Ma, Ding2; Wei, Li-Hui3; Xie, Xing1
关键词: hTERC ; ASC ; LSIL ; HR-HPV ; CIN ; Cervical cancer
刊名: GYNECOLOGIC ONCOLOGY
发表日期: 2011
DOI: 10.1016/j.ygyno.2010.10.007
卷: 120, 期:1, 页:73-83
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Obstetrics & Gynecology
研究领域[WOS]: Oncology ; Obstetrics & Gynecology
关键词[WOS]: SQUAMOUS INTRAEPITHELIAL LESIONS ; TELOMERASE RNA GENE ; ASCUS-LSIL TRIAGE ; HUMAN-PAPILLOMAVIRUS ; CERVICAL-CANCER ; PAPANICOLAOU SMEARS ; RANDOMIZED-TRIAL ; NATURAL-HISTORY ; YOUNG-WOMEN ; RISK
英文摘要:

Objective. Amplification of hTERC is found to be an important genetic event in the progression from cervical dysplasia to cervical cancer. The hTERC value in predicting high-grade cervical intraepithelial neoplasia (GIN) or squamous cell carcinoma (SCC), in high-risk HPV (HR-HPV) positive thinprep samples with atypical squamous cells (ASC) or a low-grade squamous intraepithelial lesion (LSIL) was explored in this study.

Methods. A total of 300 thinprep cytology specimens (129 of ASC-US, 82 of LSIL, and 89 of ASC-H) with positive HR-HPV DNA was detected by a two-probe dual-color FISH panel, targeting hTERC and the centromeric region of chromosome 3 (CSP3). Using >2 signals for hTERC together with 2 signals for CSP3 to define abnormal nucleus, and the cutoff value was set at 6.5 per random 200 nuclei displayed increased hTERC signals and/or tumor ploidy. Statistical analyses were based on histologic findings of colposcopy biopsies, allowing CIN2 or worse (CIN2+) as the positive criterion.

Results. The FISH results were systematically analyzed among groups, based on histologic diagnosis, cytologic finding, HR-HPV viral load, and age status. hTERC presented good consistency with histology, and had satisfactory sensitivity, specificity, and accuracy among different groups, with less difference intergroup. The individual hTERC positive nuclei ratio was generally increased with severity of the cervical lesions.

Conclusions. hTERC could be a stable predictor in assuring the risk of high-grade CIN in women with mild cytologic abnormality and positive HR-HPV, and the individual positive nuclei ratio of it might be helpful in identifying morbid grade for cervical lesions. (C) 2010 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: WKJ2007-3-001 ; WKJ2007-2-014 ; 200802109 ; 2006C13080
项目资助者: Ministry of Health ; Special Research Projects of Health Industry ; Scientific and Technologic Grant of Zhejiang Province
WOS记录号: WOS:000285824800015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52673
Appears in Collections:北京大学第二临床医学院_妇科_期刊论文

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作者单位: 1.Zhejiang Univ, Womens Hosp, Sch Med, Hangzhou 310006, Zhejiang, Peoples R China
2.Huazhong Univ Sci & Technol, Canc Biol Res Ctr, Tongji Hosp, Wuhan, Peoples R China
3.Peking Univ, Dept Gynecol, Peoples Hosp, Beijing, Peoples R China

Recommended Citation:
Li, Ying,Zeng, Wen-Jie,Ye, Feng,et al. Application of hTERC in thinprep samples with mild cytologic abnormality and HR-HPV positive[J]. GYNECOLOGIC ONCOLOGY,2011,120(1):73-83.
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