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学科主题: 口腔医学
题名:
Co-administration of aspirin and allogeneic adipose-derived stromal cells attenuates bone loss in ovariectomized rats through the anti-inflammatory and chemotactic abilities of aspirin
作者: Liu, Hao1; Li, Wei1; Liu, Yunsong2; Zhang, Xiao2; Zhou, Yongsheng2,3
关键词: Allogeneic adipose-derived stromal cells ; Aspirin ; Osteoporosis ; Bone regeneration ; Cell homing
刊名: STEM CELL RESEARCH & THERAPY
发表日期: 2015-10-16
DOI: 10.1186/s13287-015-0195-x
卷: 6
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Medicine, Research & Experimental
研究领域[WOS]: Cell Biology ; Research & Experimental Medicine
关键词[WOS]: MESENCHYMAL STEM-CELLS ; LOSS IN-VIVO ; IFN-GAMMA ; TNF-ALPHA ; T-CELLS ; MARROW ; OSTEOPOROSIS ; THERAPY ; MICE ; TRANSPLANTATION
英文摘要:

Introduction: Osteoporosis is a syndrome of excessive skeletal fragility characterized by the loss of mass and deterioration of microarchitecture in bone. Single use of aspirin or adipose-derived stromal cells (ASCs) has been recognized recently to be effective against osteoporosis. The goal of the study was to evaluate the osteogenic effects of the co-administration of aspirin and allogeneic rat adipose-derived stromal cells (rASCs) on ovariectomized (OVX)-induced bone loss in rats. The underlying mechanisms were investigated in vitro and in vivo.

Methods: Firstly, allogeneic rASCs were isolated and cultured, and the conditioned medium (CM) from the maintenance of rASCs was collected. Secondly, the OVX rats were administrated CM, rASCs, aspirin (ASP) or rASCs + ASP, respectively. Twelve weeks later, the anti-inflammatory and osteogenic effects were assessed by micro-CT, undecalcified histological sections, dynamic histomorphometric analyses and serologic assays for biochemical markers. Finally, a Transwell migration assay in vitro and cell-trafficking analyses in vivo were used to explore the effects of aspirin on rASC migration.

Results: Systemic administration of aspirin and rASCs attenuated OVX-induced bone loss better than single use of aspirin or ASCs (p < 0.05, respectively). Next, we analyzed the underlying mechanisms of the anti-inflammatory and chemotactic abilities of aspirin. Aspirin suppressed serum levels of the pro-inflammatory cytokines on tumor necrosis factor-a (TNF-a) and interferon-gamma (IFN-gamma), and the anti-inflammatory ability was positively associated with bone morphometry. Also, aspirin exhibited excellent chemotactic effects in vitro and accelerated the homing of allogeneic rASCs into bone marrow during early in vivo stages.

Conclusions: Co-administered aspirin and allogeneic ASCs can partially reverse OVX-induced bone loss in rats. This effect appears to be mediated by the anti-inflammatory and chemotactic abilities of aspirin.

语种: 英语
所属项目编号: 81170937 ; NCET-11-0026
项目资助者: National Natural Science Foundation of China ; Program for New Century Excellent Talents in University from Ministry of Education
WOS记录号: WOS:000362992500002
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52684
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Dept Prosthodont, Beijing 100081, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Natl Engn Lab Digital & Mat Technol Stomatol, Beijing 100081, Peoples R China

Recommended Citation:
Liu, Hao,Li, Wei,Liu, Yunsong,et al. Co-administration of aspirin and allogeneic adipose-derived stromal cells attenuates bone loss in ovariectomized rats through the anti-inflammatory and chemotactic abilities of aspirin[J]. STEM CELL RESEARCH &amp; THERAPY,2015,6.
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