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Co-administration of aspirin and allogeneic adipose-derived stromal cells attenuates bone loss in ovariectomized rats through the anti-inflammatory and chemotactic abilities of aspirin
Liu, Hao1; Li, Wei1; Liu, Yunsong2; Zhang, Xiao2; Zhou, Yongsheng2,3
关键词Allogeneic Adipose-derived Stromal Cells Aspirin Osteoporosis Bone Regeneration Cell Homing
刊名STEM CELL RESEARCH & THERAPY
2015-10-16
DOI10.1186/s13287-015-0195-x
6
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Medicine, Research & Experimental
研究领域[WOS]Cell Biology ; Research & Experimental Medicine
关键词[WOS]MESENCHYMAL STEM-CELLS ; LOSS IN-VIVO ; IFN-GAMMA ; TNF-ALPHA ; T-CELLS ; MARROW ; OSTEOPOROSIS ; THERAPY ; MICE ; TRANSPLANTATION
英文摘要

Introduction: Osteoporosis is a syndrome of excessive skeletal fragility characterized by the loss of mass and deterioration of microarchitecture in bone. Single use of aspirin or adipose-derived stromal cells (ASCs) has been recognized recently to be effective against osteoporosis. The goal of the study was to evaluate the osteogenic effects of the co-administration of aspirin and allogeneic rat adipose-derived stromal cells (rASCs) on ovariectomized (OVX)-induced bone loss in rats. The underlying mechanisms were investigated in vitro and in vivo.

Methods: Firstly, allogeneic rASCs were isolated and cultured, and the conditioned medium (CM) from the maintenance of rASCs was collected. Secondly, the OVX rats were administrated CM, rASCs, aspirin (ASP) or rASCs + ASP, respectively. Twelve weeks later, the anti-inflammatory and osteogenic effects were assessed by micro-CT, undecalcified histological sections, dynamic histomorphometric analyses and serologic assays for biochemical markers. Finally, a Transwell migration assay in vitro and cell-trafficking analyses in vivo were used to explore the effects of aspirin on rASC migration.

Results: Systemic administration of aspirin and rASCs attenuated OVX-induced bone loss better than single use of aspirin or ASCs (p < 0.05, respectively). Next, we analyzed the underlying mechanisms of the anti-inflammatory and chemotactic abilities of aspirin. Aspirin suppressed serum levels of the pro-inflammatory cytokines on tumor necrosis factor-a (TNF-a) and interferon-gamma (IFN-gamma), and the anti-inflammatory ability was positively associated with bone morphometry. Also, aspirin exhibited excellent chemotactic effects in vitro and accelerated the homing of allogeneic rASCs into bone marrow during early in vivo stages.

Conclusions: Co-administered aspirin and allogeneic ASCs can partially reverse OVX-induced bone loss in rats. This effect appears to be mediated by the anti-inflammatory and chemotactic abilities of aspirin.

语种英语
WOS记录号WOS:000362992500002
项目编号81170937 ; NCET-11-0026
资助机构National Natural Science Foundation of China ; Program for New Century Excellent Talents in University from Ministry of Education
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52684
专题北京大学口腔医学院
北京大学口腔医学院_口腔修复科
北京大学口腔医学院_中心实验室
作者单位1.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Dept Prosthodont, Beijing 100081, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Natl Engn Lab Digital & Mat Technol Stomatol, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Liu, Hao,Li, Wei,Liu, Yunsong,et al. Co-administration of aspirin and allogeneic adipose-derived stromal cells attenuates bone loss in ovariectomized rats through the anti-inflammatory and chemotactic abilities of aspirin[J]. STEM CELL RESEARCH &amp; THERAPY,2015,6.
APA Liu, Hao,Li, Wei,Liu, Yunsong,Zhang, Xiao,&Zhou, Yongsheng.(2015).Co-administration of aspirin and allogeneic adipose-derived stromal cells attenuates bone loss in ovariectomized rats through the anti-inflammatory and chemotactic abilities of aspirin.STEM CELL RESEARCH & THERAPY,6.
MLA Liu, Hao,et al."Co-administration of aspirin and allogeneic adipose-derived stromal cells attenuates bone loss in ovariectomized rats through the anti-inflammatory and chemotactic abilities of aspirin".STEM CELL RESEARCH & THERAPY 6(2015).
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