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学科主题基础医学
MicroRNA-19a mediates the suppressive effect of laminar flow on cyclin D1 expression in human umbilical vein endothelial cells
Qin, Xiaomei4; Wang, Xiaohong4; Wang, Ying4; Tang, Zhihui4; Cui, Qinghua4; Xi, Jianzhong5; Li, Yi-Shuan J.1,2,3; Chien, Shu1,2,3; Wang, Nanping4
关键词Cell Cycle Endothelium Flow Gene Expression Noncoding Rna
刊名PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
2010-02-16
DOI10.1073/pnas.0914882107
107期:7页:3240-3244
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]SHEAR-STRESS ; GENE-EXPRESSION ; GROWTH ; MICE ; LDL ; ANGIOGENESIS ; ACTIVATION ; MECHANISM ; PATTERN ; PROTEIN
英文摘要

Endothelial cells (ECs) respond to changes in mechanical forces, leading to the modulation of signaling networks and cell function; an example is the inhibition of EC proliferation by steady laminar flow. MicroRNAs (miRs) are short noncoding 20-22 nucleotide RNAs that negatively regulate the expression of target genes at the post-transcriptional level. This study demonstrates that miRs are involved in the flow regulation of gene expression in ECs. With the use of microRNA chip array, we found that laminar shear stress (12 dyn/cm(2), 12 h) regulated the EC expression of many miRs, including miR-19a. We further showed that stable transfection of miR-19a significantly decreased the expression of a reporter gene controlled by a conserved 3′-untranslated region of the cyclinD1 gene and also the protein level of cyclin D1, leading to an arrest of cell cycle at G1/S transition. Laminar flow suppressed cyclin D1 protein level, and this suppressive effect was diminished when the endogenous miR-19a was inhibited. In conclusion, we demonstrated that miR-19a plays an important role in the flow regulation of cyclin D1 expression. These results revealed a mechanism by which mechanical forces modulate endothelial gene expression.

语种英语
WOS记录号WOS:000274599500095
项目编号30890040 ; 30670848 ; 30821001 ; 30700292 ; 30971160 ; 2006CB503906 ; HL080518 ; HL085159
资助机构National Natural Science Foundation of China ; Major National Basic Research Program of China ; National Institutes of Health (United States Public Health Service)
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被引频次:138[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52685
专题北京大学基础医学院_心血管所
北京大学基础医学院
北京大学口腔医学院_第二门诊部
作者单位1.Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA
2.Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA
3.Univ Calif San Diego, Inst Engn Med, La Jolla, CA 92093 USA
4.Peking Univ, Hlth Sci Ctr, Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
5.Peking Univ, Coll Engn, Dept Biomed Engn, Beijing 100871, Peoples R China
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Qin, Xiaomei,Wang, Xiaohong,Wang, Ying,et al. MicroRNA-19a mediates the suppressive effect of laminar flow on cyclin D1 expression in human umbilical vein endothelial cells[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2010,107(7):3240-3244.
APA Qin, Xiaomei.,Wang, Xiaohong.,Wang, Ying.,Tang, Zhihui.,Cui, Qinghua.,...&Wang, Nanping.(2010).MicroRNA-19a mediates the suppressive effect of laminar flow on cyclin D1 expression in human umbilical vein endothelial cells.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,107(7),3240-3244.
MLA Qin, Xiaomei,et al."MicroRNA-19a mediates the suppressive effect of laminar flow on cyclin D1 expression in human umbilical vein endothelial cells".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 107.7(2010):3240-3244.
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