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学科主题: 基础医学
题名:
MicroRNA-19a mediates the suppressive effect of laminar flow on cyclin D1 expression in human umbilical vein endothelial cells
作者: Qin, Xiaomei4; Wang, Xiaohong4; Wang, Ying4; Tang, Zhihui4; Cui, Qinghua4; Xi, Jianzhong5; Li, Yi-Shuan J.1,2,3; Chien, Shu1,2,3; Wang, Nanping4
关键词: cell cycle ; endothelium ; flow ; gene expression ; noncoding RNA
刊名: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
发表日期: 2010-02-16
DOI: 10.1073/pnas.0914882107
卷: 107, 期:7, 页:3240-3244
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: SHEAR-STRESS ; GENE-EXPRESSION ; GROWTH ; MICE ; LDL ; ANGIOGENESIS ; ACTIVATION ; MECHANISM ; PATTERN ; PROTEIN
英文摘要:

Endothelial cells (ECs) respond to changes in mechanical forces, leading to the modulation of signaling networks and cell function; an example is the inhibition of EC proliferation by steady laminar flow. MicroRNAs (miRs) are short noncoding 20-22 nucleotide RNAs that negatively regulate the expression of target genes at the post-transcriptional level. This study demonstrates that miRs are involved in the flow regulation of gene expression in ECs. With the use of microRNA chip array, we found that laminar shear stress (12 dyn/cm(2), 12 h) regulated the EC expression of many miRs, including miR-19a. We further showed that stable transfection of miR-19a significantly decreased the expression of a reporter gene controlled by a conserved 3′-untranslated region of the cyclinD1 gene and also the protein level of cyclin D1, leading to an arrest of cell cycle at G1/S transition. Laminar flow suppressed cyclin D1 protein level, and this suppressive effect was diminished when the endogenous miR-19a was inhibited. In conclusion, we demonstrated that miR-19a plays an important role in the flow regulation of cyclin D1 expression. These results revealed a mechanism by which mechanical forces modulate endothelial gene expression.

语种: 英语
所属项目编号: 30890040 ; 30670848 ; 30821001 ; 30700292 ; 30971160 ; 2006CB503906 ; HL080518 ; HL085159
项目资助者: National Natural Science Foundation of China ; Major National Basic Research Program of China ; National Institutes of Health (United States Public Health Service)
WOS记录号: WOS:000274599500095
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52685
Appears in Collections:基础医学院_心血管所_期刊论文

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作者单位: 1.Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA
2.Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA
3.Univ Calif San Diego, Inst Engn Med, La Jolla, CA 92093 USA
4.Peking Univ, Hlth Sci Ctr, Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
5.Peking Univ, Coll Engn, Dept Biomed Engn, Beijing 100871, Peoples R China

Recommended Citation:
Qin, Xiaomei,Wang, Xiaohong,Wang, Ying,et al. MicroRNA-19a mediates the suppressive effect of laminar flow on cyclin D1 expression in human umbilical vein endothelial cells[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2010,107(7):3240-3244.
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