IR@PKUHSC  > 北京大学第三临床医学院  > 眼科
学科主题临床医学
Morphologic Features and Viability Analysis of Human Detached Retinal Pigment Epithelium in Age-Related Macular Degeneration
Han, Liang; Ma, Zhizhong; Wang, Changguan; Hu, Yuntao; Jin, Ying
刊名AMERICAN JOURNAL OF OPHTHALMOLOGY
2013-03-01
DOI10.1016/j.ajo.2012.09.010
155期:3页:474-483
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]BRUCHS MEMBRANE ; CLINICOPATHOLOGICAL CORRELATION ; TEARS ; TRANSPLANTATION ; TRANSLOCATION ; COMPLEX ; RABBIT
英文摘要

PURPOSE: To evaluate the morphologic features and viability of human retinal pigment epithelium (RPE) cells captured from the pigment epithelium detachment (PED) region outside the choroidal neovascular membrane lesion in eyes with hemorrhagic age-related macular degeneration.

DESIGN: Prospective, observational case series.

METHODS: Five specimens of the RPE sheet were obtained from the PED region after choroidal neovascular membrane excision in 5 eyes of 5 patients during RPE transplantation for hemorrhagic age-related macular degeneration. The specimens were stained with hematoxylin and eosin and with periodic acid-Schiff. Immunohistochemistry analysis for RPE-65 and zonula occludens-1 also was performed. RPE cells from the PED region were cultured and passaged 5 times. Scanning and transmission electron microscopy were performed to analyze the specimens.

RESULTS: The RPE cells of the specimens contained brownish pigment and were autofluorescent in vitro. Periodic acid-Schiff staining revealed that the Bruch membrane below the RPE monolayer was not intact. The specimens demonstrated positive results for both zonula occludens-1 and RPE-65 staining. The RPE basement membrane in the specimen was observed by both scanning and transmission electron microscopy. Intercellular tight junctions among RPE cells of the specimen also were observed. RPE cells captured from the PED region were cultured successfully and were passaged 5 times.

CONCLUSIONS: The RPE sheet developed from the PED region outside the choroidal neovascular membrane lesion had tight intercellular junctions, a simple RPE basement membrane, and active cellular viability. This monolayer RPE sheet may be considered as a substitution for subfoveal RPE loss in eyes with hemorrhagic age-related macular degeneration. (Am J Ophthalmol 2013;155:474-483. (C) 2013 by Elsevier Inc. All rights reserved.)

语种英语
WOS记录号WOS:000315426000009
项目编号2011CB510200
资助机构National Basic Research Program of China (973 program) ; Peking University Third Hospital, Beijing, People&prime ; s Republic of China
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52688
专题北京大学第三临床医学院_眼科
北京大学基础医学院
作者单位Peking Univ, Dept Ophthalmol, Hosp 3, Key Lab Vis Loss & Restorat,Minist Educ, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Han, Liang,Ma, Zhizhong,Wang, Changguan,et al. Morphologic Features and Viability Analysis of Human Detached Retinal Pigment Epithelium in Age-Related Macular Degeneration[J]. AMERICAN JOURNAL OF OPHTHALMOLOGY,2013,155(3):474-483.
APA Han, Liang,Ma, Zhizhong,Wang, Changguan,Hu, Yuntao,&Jin, Ying.(2013).Morphologic Features and Viability Analysis of Human Detached Retinal Pigment Epithelium in Age-Related Macular Degeneration.AMERICAN JOURNAL OF OPHTHALMOLOGY,155(3),474-483.
MLA Han, Liang,et al."Morphologic Features and Viability Analysis of Human Detached Retinal Pigment Epithelium in Age-Related Macular Degeneration".AMERICAN JOURNAL OF OPHTHALMOLOGY 155.3(2013):474-483.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Morphologic Features(2034KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Han, Liang]的文章
[Ma, Zhizhong]的文章
[Wang, Changguan]的文章
百度学术
百度学术中相似的文章
[Han, Liang]的文章
[Ma, Zhizhong]的文章
[Wang, Changguan]的文章
必应学术
必应学术中相似的文章
[Han, Liang]的文章
[Ma, Zhizhong]的文章
[Wang, Changguan]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Morphologic Features and Viability Analysis of Human Detached Retinal Pigment Epithelium in Age-Related Macular Degeneration.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。