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学科主题: 临床医学
题名:
Morphologic Features and Viability Analysis of Human Detached Retinal Pigment Epithelium in Age-Related Macular Degeneration
作者: Han, Liang; Ma, Zhizhong; Wang, Changguan; Hu, Yuntao; Jin, Ying
刊名: AMERICAN JOURNAL OF OPHTHALMOLOGY
发表日期: 2013-03-01
DOI: 10.1016/j.ajo.2012.09.010
卷: 155, 期:3, 页:474-483
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
关键词[WOS]: BRUCHS MEMBRANE ; CLINICOPATHOLOGICAL CORRELATION ; TEARS ; TRANSPLANTATION ; TRANSLOCATION ; COMPLEX ; RABBIT
英文摘要:

PURPOSE: To evaluate the morphologic features and viability of human retinal pigment epithelium (RPE) cells captured from the pigment epithelium detachment (PED) region outside the choroidal neovascular membrane lesion in eyes with hemorrhagic age-related macular degeneration.

DESIGN: Prospective, observational case series.

METHODS: Five specimens of the RPE sheet were obtained from the PED region after choroidal neovascular membrane excision in 5 eyes of 5 patients during RPE transplantation for hemorrhagic age-related macular degeneration. The specimens were stained with hematoxylin and eosin and with periodic acid-Schiff. Immunohistochemistry analysis for RPE-65 and zonula occludens-1 also was performed. RPE cells from the PED region were cultured and passaged 5 times. Scanning and transmission electron microscopy were performed to analyze the specimens.

RESULTS: The RPE cells of the specimens contained brownish pigment and were autofluorescent in vitro. Periodic acid-Schiff staining revealed that the Bruch membrane below the RPE monolayer was not intact. The specimens demonstrated positive results for both zonula occludens-1 and RPE-65 staining. The RPE basement membrane in the specimen was observed by both scanning and transmission electron microscopy. Intercellular tight junctions among RPE cells of the specimen also were observed. RPE cells captured from the PED region were cultured successfully and were passaged 5 times.

CONCLUSIONS: The RPE sheet developed from the PED region outside the choroidal neovascular membrane lesion had tight intercellular junctions, a simple RPE basement membrane, and active cellular viability. This monolayer RPE sheet may be considered as a substitution for subfoveal RPE loss in eyes with hemorrhagic age-related macular degeneration. (Am J Ophthalmol 2013;155:474-483. (C) 2013 by Elsevier Inc. All rights reserved.)

语种: 英语
所属项目编号: 2011CB510200
项目资助者: National Basic Research Program of China (973 program) ; Peking University Third Hospital, Beijing, People&prime ; s Republic of China
WOS记录号: WOS:000315426000009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52688
Appears in Collections:北京大学第三临床医学院_眼科_期刊论文

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作者单位: Peking Univ, Dept Ophthalmol, Hosp 3, Key Lab Vis Loss & Restorat,Minist Educ, Beijing 100191, Peoples R China

Recommended Citation:
Han, Liang,Ma, Zhizhong,Wang, Changguan,et al. Morphologic Features and Viability Analysis of Human Detached Retinal Pigment Epithelium in Age-Related Macular Degeneration[J]. AMERICAN JOURNAL OF OPHTHALMOLOGY,2013,155(3):474-483.
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