IR@PKUHSC  > 北京大学基础医学院  > 生理学与病理生理学系
学科主题基础医学
Conditioned immunosuppressive effect of cyclophosphamide on delayed-type hypersensitivity response and a preliminary analysis of its mechanism
Mei, L1; Li, LQ1; Li, YF1; Deng, YL1; Sun, CW1; Ding, GF1; Fan, SG1
关键词conditioning cyclophosphamide camphor 2,4-dinitrochlorobenzene hypersensitivity, delayed type
刊名NEUROIMMUNOMODULATION
2000
8期:1页:45-50
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Endocrinology & Metabolism ; Immunology ; Neurosciences
研究领域[WOS]Endocrinology & Metabolism ; Immunology ; Neurosciences & Neurology
关键词[WOS]IMMUNE-SYSTEM ; MICE
英文摘要

In the present study, camphor odor and intraperitoneal (i.p.) injection of cyclophosphamide (CY) were used as conditioned stimulus (CS) and unconditioned stimulus (US), respectively. In the unconditioned group, mice were exposed to camphor odor for 1 h followed by an i.p. injection of CY (75 mg/kg). On the next day, the above CS/US association trial session was repeated followed by smearing dinitrochlorobenzene (DNCB) on mouse abdominal skin for sensitizing the animal for delayed-type hypersensitivity (DTH) response. Five days after DNCB sensitization, mice were exposed to camphor odor(1 h), followed by an i.p. injection of CY, and then DNCB was smeared on the left ear of mice for the challenge of DTH response. Both the left/right ear weight ratio and the activity of leukocyte migration inhibitory factor (LMIF) were used as the index of DTH response, which was done 24 h after DNCB challenge. In the conditioned group, the treatment was the same as that in the unconditioned group, except that normal saline was injected on day 5 instead of CV. Furthermore, in order to analyze the mechanism of the conditioned response (CR), the mouse serum from the conditioned group (CR serum) was injected into normal mice 6 h prior to DNCB challenge. Results showed that in the conditioned group, left/right ear weight ratio and LMIF activity were statistically lower than that in the DTH group, and there was no difference between conditioned and unconditioned groups. Thus, an animal model of conditioned immunosuppressive response had been established. The results also showed that after CR serum was injected into normal mice, DTH response was also significantly suppressed. However, if CR serum was treated with dialysis (10,000 molecular weight cut-off), the suppressive effect of CR serum on DTH response disappeared. Taken together, the data suggested that a chemical compound(s) in serum, with a molecular weight less than 10,000, was important in mediating the conditioned immunosuppressive response. This may be a very important molecule(s) that could be very critical to our understanding of the interaction between the central nervous system and immune function. Copyright (C) 2000 S. Karger AG, Basel.

语种英语
WOS记录号WOS:000087829900008
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52716
Collection北京大学基础医学院_生理学与病理生理学系
作者单位1.Beijing Med Univ, Dept Physiol, Beijing 100083, Peoples R China
2.Beijing Med Univ, Dept Immunol, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Mei, L,Li, LQ,Li, YF,et al. Conditioned immunosuppressive effect of cyclophosphamide on delayed-type hypersensitivity response and a preliminary analysis of its mechanism[J]. NEUROIMMUNOMODULATION,2000,8(1):45-50.
APA Mei, L.,Li, LQ.,Li, YF.,Deng, YL.,Sun, CW.,...&Fan, SG.(2000).Conditioned immunosuppressive effect of cyclophosphamide on delayed-type hypersensitivity response and a preliminary analysis of its mechanism.NEUROIMMUNOMODULATION,8(1),45-50.
MLA Mei, L,et al."Conditioned immunosuppressive effect of cyclophosphamide on delayed-type hypersensitivity response and a preliminary analysis of its mechanism".NEUROIMMUNOMODULATION 8.1(2000):45-50.
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