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学科主题: 临床医学
题名:
Effects of transforming growth factor beta-1 infected human bone marrow mesenchymal stem cells on high- and low-metastatic potential hepatocellular carcinoma
作者: Li, Tianran1,2; Zhao, Shaohong2; Song, Bin1; Wei, Zhengmao3; Lu, Guangming4; Zhou, Jun1; Huo, Tianlong3
关键词: hMSC ; Hepatoma cells ; TGF beta-1 ; Genetically modified ; OPN ; PDCD4
刊名: EUROPEAN JOURNAL OF MEDICAL RESEARCH
发表日期: 2015-05-24
DOI: 10.1186/s40001-015-0144-2
卷: 20
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental
研究领域[WOS]: Research & Experimental Medicine
关键词[WOS]: HEPATOCYTE-LIKE CELLS ; STROMAL CELLS ; TUMOR STROMA ; RAT-LIVER ; DIFFERENTIATION ; OSTEOPONTIN ; THERAPY ; MODEL
英文摘要:

Background: This study investigates the effects of human bone marrow-derived mesenchymal stem cell (hMSC) on migration and proliferation ability of hepatocellular carcinoma (HCC) with high- and low-metastatic potential.

Methods: The hMSC and transforming growth factor-beta 1 (TGF beta-1) gene infected hMSC were co-cultured with hepatoma cells. The ability of cells migration was assessed by Transwell assay. The ability of cells proliferation was detected using CCK-8 assay. The mice were engrafted with hMSC and TGF beta-1 gene infected hMSC, respectively, after hepatoma cells inoculation 15 days, twice a week for 6 weeks successively. The tumor inhibition rate was calculated. TGF beta-1, osteopontin (OPN), and programmed cell death protein 4 (PDCD4) genes expression of hepatoma cells were detected by quantitative real-time polymerase chain reaction (qPCR) before and after co-cultured experiments.

Results: TGF beta-1 infected hMSC or hMSC co-culture with hepatoma cells groups can significantly promote hepatoma cells proliferation (P < 0.05). The migration numbers of hepatoma cells with TGF beta-1 infected hMSC co-culture groups were significantly reduced compared with the other two groups (P < 0.05). The tumors weight inhibition rates of MHCC97-H and MHCC97-L animal models were the highest in the third week by hMSC engraftment. But the highest tumor inhibition rate of MHCC97-H animal models was observed in the fourth week and MHCC97-L animal models in the fifth week after TGF beta-1 infected hMSC engraftment. OPN gene relative quantitative expression of hepatoma cells was significantly down-regulated after co-cultured with hMSC and TGF beta-1 gene infected hMSC groups (P < 0.05). TGF beta-1 gene relative quantitative expression of MHCC97-H and MHCC97-L cells was significantly up-regulated after co-cultured with TGF beta-1 gene infected hMSC groups (P < 0.05). PDCD4 expression had no statistical differences among groups.

Conclusions: hMSC and TGF beta-1 gene infected hMSC can promote hepatoma cells proliferation and inhibit hepatoma cells migration. hMSC and TGF beta-1 gene infected hMSC exhibit anti-tumor activity in a time-dependent manner. TGF beta-1 cytokine may be the main factor in HCC proliferation. OPN makes a significant contribution to the changes of hepatoma cells metastasis.

语种: 英语
所属项目编号: 81271607 ; 2013 J01392
项目资助者: National Science Foundation of China ; Natural Science Foundation of Fujian Province, China
WOS记录号: WOS:000356037100001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52719
Appears in Collections:北京大学第二临床医学院_放射科_期刊论文

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作者单位: 1.Hosp PLA 95, Dept Radiol, Putian 21000, Fujian Province, Peoples R China
2.Hosp PLA 304, Dept Radiol, Beijing 100048, Peoples R China
3.Peking Univ, Peoples Hosp, Dept Radiol, Beijing 100048, Peoples R China
4.Nanjing Gen Hosp PLA, Dept Radiol, Nanjing 21000, Jiangsu, Peoples R China

Recommended Citation:
Li, Tianran,Zhao, Shaohong,Song, Bin,et al. Effects of transforming growth factor beta-1 infected human bone marrow mesenchymal stem cells on high- and low-metastatic potential hepatocellular carcinoma[J]. EUROPEAN JOURNAL OF MEDICAL RESEARCH,2015,20.
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