北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学医药卫生分析中心  > 期刊论文
学科主题: 公共卫生
题名:
Longitudinal monitoring of tumor antiangiogenic therapy with near-infrared fluorophore-labeled agents targeted to integrin alpha(v)beta(3) and vascular endothelial growth factor
作者: Sun, Xianlei1,2; Ma, Teng1,2; Liu, Hao1,2; Yu, Xinhe1,2; Wu, Yue1,2; Shi, Jiyun1,3; Jia, Bing1,2; Zhao, Huiyun1,3; Wang, Fan1,2; Liu, Zhaofei1,2
关键词: Molecular imaging ; Angiogenesis ; Avastin ; Arginine-glycine-aspartic acid ; Tumor response
刊名: EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
发表日期: 2014-07-01
DOI: 10.1007/s00259-014-2702-1
卷: 41, 期:7, 页:1428-1439
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Radiology, Nuclear Medicine & Medical Imaging
研究领域[WOS]: Radiology, Nuclear Medicine & Medical Imaging
关键词[WOS]: F-18-FDG PET ; ANGIOGENESIS ; CANCER ; METASTASIS ; INHIBITOR ; SUNITINIB ; EFFICACY ; PEPTIDE ; CELLS ; VEGF
英文摘要:

Purpose Optical imaging is emerging as a powerful tool for the noninvasive imaging of the biological processes in living subjects. This study aimed to investigate whether optical imaging of integrin alpha(v)beta(3) and vascular endothelial growth factor (VEGF) expression can serve as sensitive biomarkers for tumor early response to antiangiogenic therapy.

Methods We synthesized two near-infrared fluorescence (NIRF) imaging agents, CF680R-3PRGD2 and CF750-BevF(ab′)(2), which were designed to specifically bind to integrin alpha(v)beta(3) and VEGF, respectively. The ability of optical imaging using the two imaging agents for early monitoring the antiangiogenic effect of sunitinib was evaluated.

Results CF680R-3PRGD2 and CF750-BevF(ab′)(2) specifically bound to their respective targets in vitro and in HT-29 tumor-bearing nude mice. Sunitinib treatment led to significantly decreased tumor uptake of CF680R-3PRGD2 (e.g., 7.47 +/- 1.62 % vs. 4.24 +/- 0.16 % on day 4; P < 0.05) and CF750-BevF(ab′)(2) (e.g., 7.43 +/- 2.43 % vs. 4.04 +/- 1.39 % on day 2; P < 0.05) in vivo. Immunofluorescence staining and an enzyme-linked immunosorbent assay confirmed that sunitinib-induced changes in tumor uptake of CF680R-3PRGD2 and CF750-BevF(ab′)(2) were correlated with changes in the levels of integrin alpha(v)beta(3) and VEGF. Radiobiodistribution of Tc-99m-3PRGD2 and I-125-BevF(ab′)(2), the radiocounterparts of CF680R-3PRGD2 and CF750-BevF(ab′)(2), respectively, also validated optical imaging results.

Conclusion Longitudinal monitoring of tumor integrin alpha(v)beta(3) and VEGF expression could be used as early biomarkers for tumor response to antiangiogenic therapy. This strategy may facilitate the development of new antiangiogenic drugs, and be used for elucidation of the underlying mechanisms of therapies involving the integrin and the VEGF signaling pathway.

语种: 英语
所属项目编号: 81222019 ; 81125011 ; 81000625 ; 2013CB733802 ; 2011CB707703 ; 2011YQ030114 ; 2012ZX09102301 ; 2012BAK25B03 ; 31300 ; BMU20110263 ; 7132131 ; 7132123 ; Z121107002512010
项目资助者: National Natural Science Foundation of China (NSFC) ; "973" projects ; Ministry of Science and Technology of China ; Ministry of Education of China ; Beijing Natural Science Foundation ; Beijing Nova Program
WOS记录号: WOS:000337286200019
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52721
Appears in Collections:北京大学医药卫生分析中心_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Med Isotopes Res Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Radiat Med, Beijing 100191, Peoples R China
3.Peking Univ, Med & Hlth Analyt Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Sun, Xianlei,Ma, Teng,Liu, Hao,et al. Longitudinal monitoring of tumor antiangiogenic therapy with near-infrared fluorophore-labeled agents targeted to integrin alpha(v)beta(3) and vascular endothelial growth factor[J]. EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING,2014,41(7):1428-1439.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Sun, Xianlei]'s Articles
[Ma, Teng]'s Articles
[Liu, Hao]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Sun, Xianlei]‘s Articles
[Ma, Teng]‘s Articles
[Liu, Hao]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace