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学科主题: 药学
题名:
Diagnostic fragment-ion-based and extension strategy coupled to DFIs intensity analysis for identification of chlorogenic acids isomers in Flos Lonicerae Japonicae by HPLC-ESI-MSn
作者: Zhang, Jia-Yu1,2; Zhang, Qian1; Li, Ning3; Wang, Zi-Jian2; Lu, Jian-Qiu2; Qiao, Yan-Jiang1
关键词: DFIBES ; HPLC-ESI-MSn ; Chlorogenic acids (CGAs) ; Isomers ; Structural characterization ; Flos Lonicerae Japonicae (FLJ)
刊名: TALANTA
发表日期: 2013-01-30
DOI: 10.1016/j.talanta.2012.11.012
卷: 104, 页:1-9
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; TRADITIONAL CHINESE MEDICINE ; POLYMETHOXYLATED FLAVONOIDS PMFS ; PHOTODIODE-ARRAY DETECTION ; LC-MSN ; STRUCTURAL-CHARACTERIZATION ; MURRAYA-PANICULATA ; PHENOLIC-COMPOUNDS ; CONSTITUENTS
英文摘要:

A method of modified diagnostic fragment-ion-based extension strategy (DFIBES) coupled to DFIs (diagnostic fragmentation ions) intensity analysis was successfully established to simultaneously screen and identify the chlorogenic acids (CGAs) in Fins Lonicerae Japonicae (FLJ) by HPLC-ESI-MSn. DFIs, such as m/z 191 [quinic acid-H](-), m/z 179 [caffeic acid-H](-) and m/z 173 [quinic acid-H-H2O](-) were determined or proposed from the fragmentation patterns analysis of corresponding reference substances for every chemical family of CGAs. A "structure extension" method was then proposed based on the well-demonstrated fragmentation patterns and was successively applied into the rapid screening of CGAs in FLJ. Considering that substitution isomerism is a common phenomenon, a full ESI-MSn fragmentation analysis according to the intensity of DFIs has been performed to identify the CGA isomers. Based on the DFIs and intensity analysis, 41 peaks attributed to CGAs including 4 caffeoylquinic acids (CQA), 7 CQA glycosides, 6 dicaffeoylquinic acids (DiCQA), 10 DiCQA glycosides, 1 tricaffeoylquinic acids (TriCQA), 4p-coumaroylquinic acids (pCoQA), 3 feruloylquinic acids (FQA) and 6 caffeoylferuloylquinic acids (CFQA) were identified preliminarily in a 65-min chromatographic run. It was the first time to systematically report the presence of CGAs in FLJ, especially for CQA glycosides, DiCQA glycosides, TriCQA, pCoQA and CFQA. All the results indicated that the method of developed DFIBES coupled to DFIs analysis was feasible, reliable and universal for screening and identifying the constituents with the same carbon skeletons especially the isomeric compounds from the complex extract of TCMs. (C) 2012 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 2010ZX09502-002
项目资助者: National S &amp ; T Major Project-Created Major New dugs Projects
WOS记录号: WOS:000319310900001
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52725
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Beijing Univ Chinese Med, Sch Chinese Pharm, Beijing 100102, Peoples R China
2.Beijing Univ Chinese Med, Ctr Sci Expt, Beijing 100029, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

Recommended Citation:
Zhang, Jia-Yu,Zhang, Qian,Li, Ning,et al. Diagnostic fragment-ion-based and extension strategy coupled to DFIs intensity analysis for identification of chlorogenic acids isomers in Flos Lonicerae Japonicae by HPLC-ESI-MSn[J]. TALANTA,2013,104:1-9.
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