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Diagnostic fragment-ion-based and extension strategy coupled to DFIs intensity analysis for identification of chlorogenic acids isomers in Flos Lonicerae Japonicae by HPLC-ESI-MSn
Zhang, Jia-Yu1,2; Zhang, Qian1; Li, Ning3; Wang, Zi-Jian2; Lu, Jian-Qiu2; Qiao, Yan-Jiang1
关键词Dfibes Hplc-esi-msn Chlorogenic Acids (Cgas) Isomers Structural Characterization Flos Lonicerae Japonicae (Flj)
刊名TALANTA
2013-01-30
DOI10.1016/j.talanta.2012.11.012
104页:1-9
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical
资助者National S &amp ; T Major Project-Created Major New dugs Projects ; National S &amp ; T Major Project-Created Major New dugs Projects
研究领域[WOS]Chemistry
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; TRADITIONAL CHINESE MEDICINE ; POLYMETHOXYLATED FLAVONOIDS PMFS ; PHOTODIODE-ARRAY DETECTION ; LC-MSN ; STRUCTURAL-CHARACTERIZATION ; MURRAYA-PANICULATA ; PHENOLIC-COMPOUNDS ; CONSTITUENTS
英文摘要

A method of modified diagnostic fragment-ion-based extension strategy (DFIBES) coupled to DFIs (diagnostic fragmentation ions) intensity analysis was successfully established to simultaneously screen and identify the chlorogenic acids (CGAs) in Fins Lonicerae Japonicae (FLJ) by HPLC-ESI-MSn. DFIs, such as m/z 191 [quinic acid-H](-), m/z 179 [caffeic acid-H](-) and m/z 173 [quinic acid-H-H2O](-) were determined or proposed from the fragmentation patterns analysis of corresponding reference substances for every chemical family of CGAs. A "structure extension" method was then proposed based on the well-demonstrated fragmentation patterns and was successively applied into the rapid screening of CGAs in FLJ. Considering that substitution isomerism is a common phenomenon, a full ESI-MSn fragmentation analysis according to the intensity of DFIs has been performed to identify the CGA isomers. Based on the DFIs and intensity analysis, 41 peaks attributed to CGAs including 4 caffeoylquinic acids (CQA), 7 CQA glycosides, 6 dicaffeoylquinic acids (DiCQA), 10 DiCQA glycosides, 1 tricaffeoylquinic acids (TriCQA), 4p-coumaroylquinic acids (pCoQA), 3 feruloylquinic acids (FQA) and 6 caffeoylferuloylquinic acids (CFQA) were identified preliminarily in a 65-min chromatographic run. It was the first time to systematically report the presence of CGAs in FLJ, especially for CQA glycosides, DiCQA glycosides, TriCQA, pCoQA and CFQA. All the results indicated that the method of developed DFIBES coupled to DFIs analysis was feasible, reliable and universal for screening and identifying the constituents with the same carbon skeletons especially the isomeric compounds from the complex extract of TCMs. (C) 2012 Elsevier B.V. All rights reserved.

语种英语
所属项目编号2010ZX09502-002
资助者National S &amp ; T Major Project-Created Major New dugs Projects ; National S &amp ; T Major Project-Created Major New dugs Projects
WOS记录号WOS:000319310900001
Citation statistics
Cited Times:43[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52725
Collection北京大学药学院
作者单位1.Beijing Univ Chinese Med, Sch Chinese Pharm, Beijing 100102, Peoples R China
2.Beijing Univ Chinese Med, Ctr Sci Expt, Beijing 100029, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Jia-Yu,Zhang, Qian,Li, Ning,et al. Diagnostic fragment-ion-based and extension strategy coupled to DFIs intensity analysis for identification of chlorogenic acids isomers in Flos Lonicerae Japonicae by HPLC-ESI-MSn[J]. TALANTA,2013,104:1-9.
APA Zhang, Jia-Yu,Zhang, Qian,Li, Ning,Wang, Zi-Jian,Lu, Jian-Qiu,&Qiao, Yan-Jiang.(2013).Diagnostic fragment-ion-based and extension strategy coupled to DFIs intensity analysis for identification of chlorogenic acids isomers in Flos Lonicerae Japonicae by HPLC-ESI-MSn.TALANTA,104,1-9.
MLA Zhang, Jia-Yu,et al."Diagnostic fragment-ion-based and extension strategy coupled to DFIs intensity analysis for identification of chlorogenic acids isomers in Flos Lonicerae Japonicae by HPLC-ESI-MSn".TALANTA 104(2013):1-9.
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