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学科主题: 基础医学
题名:
Assessment of shikonin for potential estrogenic activity by dual-luciferase reporter based bioluminescent measurements in vitro
作者: Zhang, Yi2,3; Li, Ping-Ping1
刊名: PHARMAZIE
发表日期: 2011-02-01
DOI: 10.1691/ph.2011.0714
卷: 66, 期:2, 页:141-143
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Medicinal ; Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy ; Chemistry
关键词[WOS]: STEROID SULFATASE GENES ; LITHOSPERMUM-ERYTHRORHIZON ; CELLS ; PHARMACOLOGY ; ACTIVATION ; MECHANISMS ; EXPRESSION ; EXTRACT ; CANCER
英文摘要:

Shikonin, an active component of Lithospermum erythrorhizon Sieb. et Zucc., shows multiple pharmacological properties. However, the estrogenic activity of shikonin is remaining unclear. We assessed the potential estrogenic activity of shikonin with dual-luciferase reporter assay and bioluminescent measurements, by using transient cotransfection with estrogen dependent plasmid pERE-TK-Luc and internal control plasmid pRL-TK in MCF-7 cells. Estrogenic activity of shikonin, even at high concentration did not alter significantly compared to negative control (p > 0.05) and were significantly lower than those with E-2 (p < 0.01). Concluding, shikonin demonstrates no estrogenic activity in vitro.

语种: 英语
所属项目编号: 2004-IV15
项目资助者: Key Program Foundation of Beijing Administration of TCMs, China
WOS记录号: WOS:000288052700010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52729
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Dept Integrated Tradit Chinese & Western Med, Key Lab Carcinogenesis & Translat Res,Minist Educ, Sch Oncol,Beijing Canc Hosp & Inst, Beijing 100142, Peoples R China
2.Beijing Univ Chinese Med, Dept Anat, Sch Preclin Med, Beijing, Peoples R China
3.Peking Univ, Dept Integrated Tradit Chinese & Western Med, Sch Basic Med Sci, Beijing 100142, Peoples R China

Recommended Citation:
Zhang, Yi,Li, Ping-Ping. Assessment of shikonin for potential estrogenic activity by dual-luciferase reporter based bioluminescent measurements in vitro[J]. PHARMAZIE,2011,66(2):141-143.
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