|Decrease in the production of beta-amyloid by berberine inhibition of the expression of beta-secretase in HEK293 cells|
|Zhu, Feiqi1,2; Wu, Fujun3; Ma, Ying4; Liu, Guangjian2; Li, Zhong5; Sun, Yong&prime1; an6; Pei, Zhong5|
|关键词||Alzheimer&Prime s Disease Berberine Beta-amyloid(40/42) Beta-secretase Extracellular Signal-regulated Kinase1/2|
|WOS标题词||Science & Technology|
|研究领域[WOS]||Neurosciences & Neurology|
|关键词[WOS]||NONSTEROIDAL ANTIINFLAMMATORY DRUGS ; ALZHEIMERS-DISEASE ; IN-VITRO ; COPTIDIS-RHIZOMA ; CANCER-CELLS ; GLUCOSE-METABOLISM ; MECHANISM DISTINCT ; PRECURSOR PROTEIN ; NITRIC-OXIDE ; RAT MODEL|
Background: Berberine (BER), the major alkaloidal component of Rhizoma coptidis, has multiple pharmacological effects including inhibition of acetylcholinesterase, reduction of cholesterol and glucose levels, anti-inflammatory, neuroprotective and neurotrophic effects. It has also been demonstrated that BER can reduce the production of beta-amyloid(40/42), which plays a critical and primary role in the pathogenesis of Alzheimer′s disease. However, the mechanism by which it accomplishes this remains unclear.
Results: Here, we report that BER could not only significantly decrease the production of beta-amyloid40/42 and the expression of beta-secretase (BACE), but was also able to activate the extracellular signal-regulated kinase1/2 (ERK1/2) pathway in a dose-and time-dependent manner in HEK293 cells stably transfected with APP695 containing the Swedish mutation. We also find that U0126, an antagonist of the ERK1/2 pathway, could abolish (1) the activation activity of BER on the ERK1/2 pathway and (2) the inhibition activity of BER on the production of beta-amyloid(40/42) and the expression of BACE.
Conclusion: Our data indicate that BER decreases the production of beta-amyloid(40/42) by inhibiting the expression of BACE via activation of the ERK1/2 pathway.
|资助机构||National Natural Science Foundation of China|
|作者单位||1.Shantou Univ, Coll Med, Dept Neurol, Affiliated Yuebei Peoples Hosp, Shaoguan 512026, Guangdong, Peoples R China|
2.Hubei Univ Med, Affiliated Hosp, Dept Neurol, Taihe Hosp, Shiyan 442000, Hubei, Peoples R China
3.Anhui Agr Univ, Coll Life Sci, Hefei 230036, Anhui, Peoples R China
4.Shantou Univ, Coll Med, Dept Cardiol, Affiliated Yuebei Peoples Hosp, Shaoguan 512026, Guangdong, Peoples R China
5.Sun Yat Sen Univ, Affiliated Hosp 1, Dept Neurol, Guangzhou 510080, Guangdong, Peoples R China
6.Peking Univ, Hosp 1, Dept Neurol, Beijing 100034, Peoples R China
|Zhu, Feiqi,Wu, Fujun,Ma, Ying,et al. Decrease in the production of beta-amyloid by berberine inhibition of the expression of beta-secretase in HEK293 cells[J]. BMC NEUROSCIENCE,2011,12.|
|APA||Zhu, Feiqi.,Wu, Fujun.,Ma, Ying.,Liu, Guangjian.,Li, Zhong.,...&Pei, Zhong.(2011).Decrease in the production of beta-amyloid by berberine inhibition of the expression of beta-secretase in HEK293 cells.BMC NEUROSCIENCE,12.|
|MLA||Zhu, Feiqi,et al."Decrease in the production of beta-amyloid by berberine inhibition of the expression of beta-secretase in HEK293 cells".BMC NEUROSCIENCE 12(2011).|